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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-36549127-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=36549127&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 36549127,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_006097.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "NM_006097.5",
          "protein_id": "NP_006088.2",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000279022.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006097.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000279022.7",
          "protein_id": "ENSP00000279022.2",
          "transcript_support_level": 1,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006097.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000279022.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.235C>T",
          "hgvs_p": "p.Arg79Cys",
          "transcript": "ENST00000346786.2",
          "protein_id": "ENSP00000217313.2",
          "transcript_support_level": 1,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 118,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 357,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000346786.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "DLGAP4-AS1",
          "gene_hgnc_id": 51223,
          "hgvs_c": "n.467+22314G>A",
          "hgvs_p": null,
          "transcript": "ENST00000439595.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000439595.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.421C>T",
          "hgvs_p": "p.Arg141Cys",
          "transcript": "ENST00000866578.1",
          "protein_id": "ENSP00000536637.1",
          "transcript_support_level": null,
          "aa_start": 141,
          "aa_end": null,
          "aa_length": 180,
          "cds_start": 421,
          "cds_end": null,
          "cds_length": 543,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866578.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866572.1",
          "protein_id": "ENSP00000536631.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866572.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866573.1",
          "protein_id": "ENSP00000536632.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866573.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866574.1",
          "protein_id": "ENSP00000536633.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866574.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866575.1",
          "protein_id": "ENSP00000536634.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866575.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866577.1",
          "protein_id": "ENSP00000536636.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866577.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866579.1",
          "protein_id": "ENSP00000536638.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866579.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866580.1",
          "protein_id": "ENSP00000536639.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866580.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866581.1",
          "protein_id": "ENSP00000536640.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866581.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866582.1",
          "protein_id": "ENSP00000536641.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866582.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000866583.1",
          "protein_id": "ENSP00000536642.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866583.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys",
          "transcript": "ENST00000946382.1",
          "protein_id": "ENSP00000616441.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000946382.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.382C>T",
          "hgvs_p": "p.Arg128Cys",
          "transcript": "ENST00000866576.1",
          "protein_id": "ENSP00000536635.1",
          "transcript_support_level": null,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 167,
          "cds_start": 382,
          "cds_end": null,
          "cds_length": 504,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000866576.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYL9",
          "gene_hgnc_id": 15754,
          "hgvs_c": "c.235C>T",
          "hgvs_p": "p.Arg79Cys",
          "transcript": "NM_181526.3",
          "protein_id": "NP_852667.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 118,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 357,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_181526.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "DLGAP4-AS1",
          "gene_hgnc_id": 51223,
          "hgvs_c": "n.603-21203G>A",
          "hgvs_p": null,
          "transcript": "ENST00000425233.7",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000425233.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "DLGAP4-AS1",
          "gene_hgnc_id": 51223,
          "hgvs_c": "n.576+22314G>A",
          "hgvs_p": null,
          "transcript": "ENST00000793269.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000793269.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
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        {
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        },
        {
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          "protein_coding": false,
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            "intron_variant"
          ],
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          "gene_symbol": "DLGAP4-AS1",
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          "hgvs_c": "n.467+22314G>A",
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          "transcript": "NR_109939.1",
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          "biotype": "pseudogene",
          "feature": "NR_109939.1"
        }
      ],
      "gene_symbol": "MYL9",
      "gene_hgnc_id": 15754,
      "dbsnp": "rs771760122",
      "frequency_reference_population": 0.0000049579257,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 8,
      "gnomad_exomes_af": 0.00000478981,
      "gnomad_genomes_af": 0.00000657281,
      "gnomad_exomes_ac": 7,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7356607913970947,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.588,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9071,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.017,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_006097.5",
          "gene_symbol": "MYL9",
          "hgnc_id": 15754,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133Cys"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000439595.5",
          "gene_symbol": "DLGAP4-AS1",
          "hgnc_id": 51223,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.467+22314G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}