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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-38525910-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=38525910&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 38525910,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000262879.11",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "NM_020336.4",
          "protein_id": "NP_065069.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1494,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4485,
          "cdna_start": 2175,
          "cdna_end": null,
          "cdna_length": 8633,
          "mane_select": "ENST00000262879.11",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "ENST00000262879.11",
          "protein_id": "ENSP00000262879.6",
          "transcript_support_level": 1,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1494,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4485,
          "cdna_start": 2175,
          "cdna_end": null,
          "cdna_length": 8633,
          "mane_select": "NM_020336.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "ENST00000397040.5",
          "protein_id": "ENSP00000380233.1",
          "transcript_support_level": 1,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1494,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4485,
          "cdna_start": 1976,
          "cdna_end": null,
          "cdna_length": 8435,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "ENST00000397042.7",
          "protein_id": "ENSP00000380235.3",
          "transcript_support_level": 1,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1491,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4476,
          "cdna_start": 2202,
          "cdna_end": null,
          "cdna_length": 8652,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "NM_001282917.2",
          "protein_id": "NP_001269846.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1494,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4485,
          "cdna_start": 2033,
          "cdna_end": null,
          "cdna_length": 8491,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "NM_001282918.2",
          "protein_id": "NP_001269847.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1491,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4476,
          "cdna_start": 2175,
          "cdna_end": null,
          "cdna_length": 8624,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1402A>G",
          "hgvs_p": "p.Lys468Glu",
          "transcript": "ENST00000438490.2",
          "protein_id": "ENSP00000416646.1",
          "transcript_support_level": 5,
          "aa_start": 468,
          "aa_end": null,
          "aa_length": 1323,
          "cds_start": 1402,
          "cds_end": null,
          "cds_length": 3972,
          "cdna_start": 1402,
          "cdna_end": null,
          "cdna_length": 4075,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.19A>G",
          "hgvs_p": "p.Lys7Glu",
          "transcript": "ENST00000632792.1",
          "protein_id": "ENSP00000487816.1",
          "transcript_support_level": 5,
          "aa_start": 7,
          "aa_end": null,
          "aa_length": 865,
          "cds_start": 19,
          "cds_end": null,
          "cds_length": 2598,
          "cdna_start": 20,
          "cdna_end": null,
          "cdna_length": 4858,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Lys652Glu",
          "transcript": "XM_005260462.4",
          "protein_id": "XP_005260519.1",
          "transcript_support_level": null,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 1507,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 4524,
          "cdna_start": 1984,
          "cdna_end": null,
          "cdna_length": 8445,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Lys652Glu",
          "transcript": "XM_047440314.1",
          "protein_id": "XP_047296270.1",
          "transcript_support_level": null,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 1506,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 4521,
          "cdna_start": 1984,
          "cdna_end": null,
          "cdna_length": 8442,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Lys652Glu",
          "transcript": "XM_047440315.1",
          "protein_id": "XP_047296271.1",
          "transcript_support_level": null,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 1503,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 4512,
          "cdna_start": 1984,
          "cdna_end": null,
          "cdna_length": 8433,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Lys652Glu",
          "transcript": "XM_017027966.3",
          "protein_id": "XP_016883455.1",
          "transcript_support_level": null,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 1502,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 4509,
          "cdna_start": 1984,
          "cdna_end": null,
          "cdna_length": 8430,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "XM_005260465.4",
          "protein_id": "XP_005260522.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1495,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4488,
          "cdna_start": 1948,
          "cdna_end": null,
          "cdna_length": 8409,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu",
          "transcript": "XM_017027967.3",
          "protein_id": "XP_016883456.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 1490,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 4473,
          "cdna_start": 1948,
          "cdna_end": null,
          "cdna_length": 8394,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1600A>G",
          "hgvs_p": "p.Lys534Glu",
          "transcript": "XM_017027968.3",
          "protein_id": "XP_016883457.1",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 1389,
          "cds_start": 1600,
          "cds_end": null,
          "cds_length": 4170,
          "cdna_start": 1722,
          "cdna_end": null,
          "cdna_length": 8183,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Lys652Glu",
          "transcript": "XM_047440316.1",
          "protein_id": "XP_047296272.1",
          "transcript_support_level": null,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 1335,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 4008,
          "cdna_start": 1984,
          "cdna_end": null,
          "cdna_length": 4146,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RALGAPB",
          "gene_hgnc_id": 29221,
          "hgvs_c": "n.1170A>G",
          "hgvs_p": null,
          "transcript": "ENST00000461423.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3830,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RALGAPB",
      "gene_hgnc_id": 29221,
      "dbsnp": "rs546763036",
      "frequency_reference_population": 0.0000062027198,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 10,
      "gnomad_exomes_af": 0.00000410999,
      "gnomad_genomes_af": 0.0000262574,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2352844774723053,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.398,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.6602,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.3,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.942,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000262879.11",
          "gene_symbol": "RALGAPB",
          "hgnc_id": 29221,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1918A>G",
          "hgvs_p": "p.Lys640Glu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}