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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-417906-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=417906&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 417906,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000356286.10",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.436G>C",
          "hgvs_p": "p.Glu146Gln",
          "transcript": "NM_031229.4",
          "protein_id": "NP_112506.2",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 436,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 3701,
          "mane_select": "ENST00000356286.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.436G>C",
          "hgvs_p": "p.Glu146Gln",
          "transcript": "ENST00000356286.10",
          "protein_id": "ENSP00000348632.6",
          "transcript_support_level": 1,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 436,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 3701,
          "mane_select": "NM_031229.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.310G>C",
          "hgvs_p": "p.Glu104Gln",
          "transcript": "ENST00000353660.7",
          "protein_id": "ENSP00000254960.5",
          "transcript_support_level": 1,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 310,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": 878,
          "cdna_end": null,
          "cdna_length": 2511,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.310G>C",
          "hgvs_p": null,
          "transcript": "ENST00000382181.2",
          "protein_id": "ENSP00000371616.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2208,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.436G>C",
          "hgvs_p": null,
          "transcript": "ENST00000382214.7",
          "protein_id": "ENSP00000371649.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2752,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.487G>C",
          "hgvs_p": "p.Glu163Gln",
          "transcript": "NM_001410770.1",
          "protein_id": "NP_001397699.1",
          "transcript_support_level": null,
          "aa_start": 163,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 487,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": 946,
          "cdna_end": null,
          "cdna_length": 3752,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.487G>C",
          "hgvs_p": "p.Glu163Gln",
          "transcript": "ENST00000697805.1",
          "protein_id": "ENSP00000513446.1",
          "transcript_support_level": null,
          "aa_start": 163,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 487,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": 741,
          "cdna_end": null,
          "cdna_length": 2360,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.310G>C",
          "hgvs_p": "p.Glu104Gln",
          "transcript": "NM_006462.6",
          "protein_id": "NP_006453.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 310,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": 750,
          "cdna_end": null,
          "cdna_length": 3556,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.532G>C",
          "hgvs_p": "p.Glu178Gln",
          "transcript": "ENST00000414880.1",
          "protein_id": "ENSP00000393841.1",
          "transcript_support_level": 3,
          "aa_start": 178,
          "aa_end": null,
          "aa_length": 253,
          "cds_start": 532,
          "cds_end": null,
          "cds_length": 763,
          "cdna_start": 534,
          "cdna_end": null,
          "cdna_length": 765,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.610G>C",
          "hgvs_p": "p.Glu204Gln",
          "transcript": "XM_011529137.3",
          "protein_id": "XP_011527439.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": 1069,
          "cdna_end": null,
          "cdna_length": 3875,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.559G>C",
          "hgvs_p": "p.Glu187Gln",
          "transcript": "XM_011529138.2",
          "protein_id": "XP_011527440.1",
          "transcript_support_level": null,
          "aa_start": 187,
          "aa_end": null,
          "aa_length": 551,
          "cds_start": 559,
          "cds_end": null,
          "cds_length": 1656,
          "cdna_start": 1018,
          "cdna_end": null,
          "cdna_length": 3824,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.610G>C",
          "hgvs_p": "p.Glu204Gln",
          "transcript": "XM_011529139.4",
          "protein_id": "XP_011527441.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": 1069,
          "cdna_end": null,
          "cdna_length": 2042,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.610G>C",
          "hgvs_p": "p.Glu204Gln",
          "transcript": "XM_011529140.3",
          "protein_id": "XP_011527442.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": 1069,
          "cdna_end": null,
          "cdna_length": 2135,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.610G>C",
          "hgvs_p": "p.Glu204Gln",
          "transcript": "XM_047439833.1",
          "protein_id": "XP_047295789.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 330,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 993,
          "cdna_start": 1069,
          "cdna_end": null,
          "cdna_length": 1501,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.436G>C",
          "hgvs_p": "p.Glu146Gln",
          "transcript": "XM_047439834.1",
          "protein_id": "XP_047295790.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": 436,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 1961,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.487G>C",
          "hgvs_p": "p.Glu163Gln",
          "transcript": "XM_047439835.1",
          "protein_id": "XP_047295791.1",
          "transcript_support_level": null,
          "aa_start": 163,
          "aa_end": null,
          "aa_length": 289,
          "cds_start": 487,
          "cds_end": null,
          "cds_length": 870,
          "cdna_start": 946,
          "cdna_end": null,
          "cdna_length": 1378,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.87G>C",
          "hgvs_p": "p.Gly29Gly",
          "transcript": "NM_001323956.2",
          "protein_id": "NP_001310885.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 3540,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.87G>C",
          "hgvs_p": "p.Gly29Gly",
          "transcript": "NM_001323958.2",
          "protein_id": "NP_001310887.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": 750,
          "cdna_end": null,
          "cdna_length": 3395,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.436G>C",
          "hgvs_p": null,
          "transcript": "ENST00000415942.5",
          "protein_id": "ENSP00000405288.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2613,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.436G>C",
          "hgvs_p": null,
          "transcript": "ENST00000697804.1",
          "protein_id": "ENSP00000513444.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2671,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.895G>C",
          "hgvs_p": null,
          "transcript": "NR_136659.2",
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        },
        {
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          "protein_coding": true,
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            "downstream_gene_variant"
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          "exon_count": 5,
          "intron_rank": null,
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          "hgvs_c": "c.*84G>C",
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          "transcript": "ENST00000411647.5",
          "protein_id": "ENSP00000415080.1",
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          "cds_start": -4,
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          "cdna_start": null,
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        },
        {
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          "canonical": false,
          "protein_coding": true,
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          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "RBCK1",
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          "hgvs_c": "c.*83G>C",
          "hgvs_p": null,
          "transcript": "ENST00000441733.5",
          "protein_id": "ENSP00000387799.1",
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          "aa_start": null,
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          "cds_start": -4,
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          "cds_length": 350,
          "cdna_start": null,
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          "cdna_length": 961,
          "mane_select": null,
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          "feature": null
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      ],
      "gene_symbol": "RBCK1",
      "gene_hgnc_id": 15864,
      "dbsnp": "rs1555785275",
      "frequency_reference_population": 0.0000013717685,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000137177,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07192486524581909,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.034,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0694,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.65,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.132,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000356286.10",
          "gene_symbol": "RBCK1",
          "hgnc_id": 15864,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.436G>C",
          "hgvs_p": "p.Glu146Gln"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}