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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-428988-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=428988&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 428988,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001410770.1",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1346A>C",
          "hgvs_p": "p.Gln449Pro",
          "transcript": "NM_031229.4",
          "protein_id": "NP_112506.2",
          "transcript_support_level": null,
          "aa_start": 449,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 1346,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000356286.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031229.4"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1346A>C",
          "hgvs_p": "p.Gln449Pro",
          "transcript": "ENST00000356286.10",
          "protein_id": "ENSP00000348632.6",
          "transcript_support_level": 1,
          "aa_start": 449,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 1346,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_031229.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000356286.10"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1220A>C",
          "hgvs_p": "p.Gln407Pro",
          "transcript": "ENST00000353660.7",
          "protein_id": "ENSP00000254960.5",
          "transcript_support_level": 1,
          "aa_start": 407,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 1220,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000353660.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.*366A>C",
          "hgvs_p": null,
          "transcript": "ENST00000382181.2",
          "protein_id": "ENSP00000371616.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000382181.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.*310A>C",
          "hgvs_p": null,
          "transcript": "ENST00000382214.7",
          "protein_id": "ENSP00000371649.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000382214.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.*366A>C",
          "hgvs_p": null,
          "transcript": "ENST00000382181.2",
          "protein_id": "ENSP00000371616.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000382181.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "n.*310A>C",
          "hgvs_p": null,
          "transcript": "ENST00000382214.7",
          "protein_id": "ENSP00000371649.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000382214.7"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1514A>C",
          "hgvs_p": "p.Gln505Pro",
          "transcript": "ENST00000952173.1",
          "protein_id": "ENSP00000622232.1",
          "transcript_support_level": null,
          "aa_start": 505,
          "aa_end": null,
          "aa_length": 566,
          "cds_start": 1514,
          "cds_end": null,
          "cds_length": 1701,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952173.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1466A>C",
          "hgvs_p": "p.Gln489Pro",
          "transcript": "ENST00000952176.1",
          "protein_id": "ENSP00000622235.1",
          "transcript_support_level": null,
          "aa_start": 489,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 1466,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952176.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1463A>C",
          "hgvs_p": "p.Gln488Pro",
          "transcript": "ENST00000878596.1",
          "protein_id": "ENSP00000548655.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 549,
          "cds_start": 1463,
          "cds_end": null,
          "cds_length": 1650,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878596.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1460A>C",
          "hgvs_p": "p.Gln487Pro",
          "transcript": "ENST00000952178.1",
          "protein_id": "ENSP00000622237.1",
          "transcript_support_level": null,
          "aa_start": 487,
          "aa_end": null,
          "aa_length": 548,
          "cds_start": 1460,
          "cds_end": null,
          "cds_length": 1647,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952178.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1457A>C",
          "hgvs_p": "p.Gln486Pro",
          "transcript": "ENST00000952172.1",
          "protein_id": "ENSP00000622231.1",
          "transcript_support_level": null,
          "aa_start": 486,
          "aa_end": null,
          "aa_length": 547,
          "cds_start": 1457,
          "cds_end": null,
          "cds_length": 1644,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952172.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1448A>C",
          "hgvs_p": "p.Gln483Pro",
          "transcript": "ENST00000952177.1",
          "protein_id": "ENSP00000622236.1",
          "transcript_support_level": null,
          "aa_start": 483,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 1448,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952177.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1430A>C",
          "hgvs_p": "p.Gln477Pro",
          "transcript": "ENST00000932273.1",
          "protein_id": "ENSP00000602332.1",
          "transcript_support_level": null,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 1430,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932273.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1397A>C",
          "hgvs_p": "p.Gln466Pro",
          "transcript": "NM_001410770.1",
          "protein_id": "NP_001397699.1",
          "transcript_support_level": null,
          "aa_start": 466,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 1397,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410770.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1397A>C",
          "hgvs_p": "p.Gln466Pro",
          "transcript": "ENST00000697805.1",
          "protein_id": "ENSP00000513446.1",
          "transcript_support_level": null,
          "aa_start": 466,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 1397,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697805.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1385A>C",
          "hgvs_p": "p.Gln462Pro",
          "transcript": "ENST00000952174.1",
          "protein_id": "ENSP00000622233.1",
          "transcript_support_level": null,
          "aa_start": 462,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 1385,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952174.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1361A>C",
          "hgvs_p": "p.Gln454Pro",
          "transcript": "ENST00000952175.1",
          "protein_id": "ENSP00000622234.1",
          "transcript_support_level": null,
          "aa_start": 454,
          "aa_end": null,
          "aa_length": 515,
          "cds_start": 1361,
          "cds_end": null,
          "cds_length": 1548,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952175.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1343A>C",
          "hgvs_p": "p.Gln448Pro",
          "transcript": "ENST00000878601.1",
          "protein_id": "ENSP00000548660.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 509,
          "cds_start": 1343,
          "cds_end": null,
          "cds_length": 1530,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878601.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBCK1",
          "gene_hgnc_id": 15864,
          "hgvs_c": "c.1340A>C",
          "hgvs_p": "p.Gln447Pro",
          "transcript": "ENST00000952170.1",
          "protein_id": "ENSP00000622229.1",
          "transcript_support_level": null,
          "aa_start": 447,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1340,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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        {
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        {
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          ],
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000680792.1"
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      ],
      "gene_symbol": "RBCK1",
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      "dbsnp": "rs371077650",
      "frequency_reference_population": 6.8522814e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.85228e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.35268375277519226,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.18000000715255737,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.245,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.306,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.15,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.708,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.18,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001410770.1",
          "gene_symbol": "RBCK1",
          "hgnc_id": 15864,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1397A>C",
          "hgvs_p": "p.Gln466Pro"
        },
        {
          "score": 1,
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          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000680792.1",
          "gene_symbol": "TBC1D20",
          "hgnc_id": 16133,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "n.*196-4714T>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}