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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-438655-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=438655&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 438655,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "ENST00000354200.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.1143G>A",
          "hgvs_p": "p.Ala381Ala",
          "transcript": "NM_144628.4",
          "protein_id": "NP_653229.1",
          "transcript_support_level": null,
          "aa_start": 381,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1143,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": 1271,
          "cdna_end": null,
          "cdna_length": 4446,
          "mane_select": "ENST00000354200.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.1143G>A",
          "hgvs_p": "p.Ala381Ala",
          "transcript": "ENST00000354200.5",
          "protein_id": "ENSP00000346139.4",
          "transcript_support_level": 1,
          "aa_start": 381,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1143,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": 1271,
          "cdna_end": null,
          "cdna_length": 4446,
          "mane_select": "NM_144628.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1143G>A",
          "hgvs_p": null,
          "transcript": "ENST00000461304.5",
          "protein_id": "ENSP00000432280.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2024,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.1239G>A",
          "hgvs_p": "p.Ala413Ala",
          "transcript": "ENST00000679895.1",
          "protein_id": "ENSP00000505197.1",
          "transcript_support_level": null,
          "aa_start": 413,
          "aa_end": null,
          "aa_length": 435,
          "cds_start": 1239,
          "cds_end": null,
          "cds_length": 1308,
          "cdna_start": 1373,
          "cdna_end": null,
          "cdna_length": 4531,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.1143G>A",
          "hgvs_p": "p.Ala381Ala",
          "transcript": "ENST00000681539.1",
          "protein_id": "ENSP00000505557.1",
          "transcript_support_level": null,
          "aa_start": 381,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1143,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": 1288,
          "cdna_end": null,
          "cdna_length": 3506,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.1068G>A",
          "hgvs_p": "p.Ala356Ala",
          "transcript": "ENST00000681551.1",
          "protein_id": "ENSP00000504974.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 1068,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": 1202,
          "cdna_end": null,
          "cdna_length": 4355,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.1062G>A",
          "hgvs_p": "p.Ala354Ala",
          "transcript": "ENST00000681414.1",
          "protein_id": "ENSP00000505797.1",
          "transcript_support_level": null,
          "aa_start": 354,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 1062,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": 1173,
          "cdna_end": null,
          "cdna_length": 4336,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.957G>A",
          "hgvs_p": "p.Ala319Ala",
          "transcript": "ENST00000680050.1",
          "protein_id": "ENSP00000505464.1",
          "transcript_support_level": null,
          "aa_start": 319,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 957,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": 1068,
          "cdna_end": null,
          "cdna_length": 2479,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "c.957G>A",
          "hgvs_p": "p.Ala319Ala",
          "transcript": "ENST00000681129.1",
          "protein_id": "ENSP00000505329.1",
          "transcript_support_level": null,
          "aa_start": 319,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 957,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": 1071,
          "cdna_end": null,
          "cdna_length": 4234,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.2383G>A",
          "hgvs_p": null,
          "transcript": "ENST00000461188.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5541,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1448G>A",
          "hgvs_p": null,
          "transcript": "ENST00000494633.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3820,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1337G>A",
          "hgvs_p": null,
          "transcript": "ENST00000679451.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4495,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1143G>A",
          "hgvs_p": null,
          "transcript": "ENST00000679741.1",
          "protein_id": "ENSP00000504904.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2708,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1143G>A",
          "hgvs_p": null,
          "transcript": "ENST00000679944.1",
          "protein_id": "ENSP00000506278.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2846,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.2032G>A",
          "hgvs_p": null,
          "transcript": "ENST00000679953.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5190,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1071G>A",
          "hgvs_p": null,
          "transcript": "ENST00000679973.1",
          "protein_id": "ENSP00000506502.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4095,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1288G>A",
          "hgvs_p": null,
          "transcript": "ENST00000680088.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4451,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1143G>A",
          "hgvs_p": null,
          "transcript": "ENST00000680106.1",
          "protein_id": "ENSP00000505500.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.1143G>A",
          "hgvs_p": null,
          "transcript": "ENST00000680284.1",
          "protein_id": "ENSP00000506231.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2982,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.2634G>A",
          "hgvs_p": null,
          "transcript": "ENST00000680491.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5792,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TBC1D20",
          "gene_hgnc_id": 16133,
          "hgvs_c": "n.957G>A",
          "hgvs_p": null,
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      ],
      "gene_symbol": "TBC1D20",
      "gene_hgnc_id": 16133,
      "dbsnp": "rs141648576",
      "frequency_reference_population": 0.0006015416,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 971,
      "gnomad_exomes_af": 0.000625903,
      "gnomad_genomes_af": 0.0003677,
      "gnomad_exomes_ac": 915,
      "gnomad_genomes_ac": 56,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.6399999856948853,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.64,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.308,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6,BP7",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000354200.5",
          "gene_symbol": "TBC1D20",
          "hgnc_id": 16133,
          "effects": [
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          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1143G>A",
          "hgvs_p": "p.Ala381Ala"
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      ],
      "clinvar_disease": "not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:2",
      "phenotype_combined": "not specified|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}