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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-44480156-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=44480156&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 44480156,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_024331.5",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "NM_001039199.3",
          "protein_id": "NP_001034288.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262605.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001039199.3"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000262605.9",
          "protein_id": "ENSP00000262605.4",
          "transcript_support_level": 1,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001039199.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262605.9"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000372904.7",
          "protein_id": "ENSP00000361995.3",
          "transcript_support_level": 1,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000372904.7"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "NM_024331.5",
          "protein_id": "NP_077307.2",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024331.5"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901707.1",
          "protein_id": "ENSP00000571766.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901707.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901708.1",
          "protein_id": "ENSP00000571767.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901708.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901712.1",
          "protein_id": "ENSP00000571771.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901712.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901715.1",
          "protein_id": "ENSP00000571774.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901715.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901717.1",
          "protein_id": "ENSP00000571776.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901717.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901721.1",
          "protein_id": "ENSP00000571780.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901721.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901722.1",
          "protein_id": "ENSP00000571781.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901722.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901723.1",
          "protein_id": "ENSP00000571782.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901723.1"
        },
        {
          "aa_ref": "W",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901726.1",
          "protein_id": "ENSP00000571785.1",
          "transcript_support_level": null,
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          "cds_start": 157,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000901726.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000945911.1",
          "protein_id": "ENSP00000615970.1",
          "transcript_support_level": null,
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          "cds_start": 157,
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          "cdna_start": null,
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          "mane_select": null,
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        },
        {
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          ],
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          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901720.1",
          "protein_id": "ENSP00000571779.1",
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          "cds_start": 157,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000901720.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901714.1",
          "protein_id": "ENSP00000571773.1",
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          "aa_end": null,
          "aa_length": 320,
          "cds_start": 157,
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          "cds_length": 963,
          "cdna_start": null,
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        },
        {
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          "strand": true,
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "NM_001261839.2",
          "protein_id": "NP_001248768.1",
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          "cds_start": 157,
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        {
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          "intron_rank": null,
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          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
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          "transcript": "ENST00000901709.1",
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          "biotype": "protein_coding",
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        },
        {
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          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
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          "transcript": "ENST00000901710.1",
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000901710.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTPAL",
          "gene_hgnc_id": 16114,
          "hgvs_c": "c.157T>C",
          "hgvs_p": "p.Trp53Arg",
          "transcript": "ENST00000901719.1",
          "protein_id": "ENSP00000571778.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 157,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000901719.1"
        },
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      ],
      "gene_symbol": "TTPAL",
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      "dbsnp": null,
      "frequency_reference_population": 0.0000020521547,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205215,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2652643918991089,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.552,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9969,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 4.613,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_024331.5",
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          "effects": [
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          "inheritance_mode": "AR",
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          "hgvs_p": "p.Trp53Arg"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}