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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-44622911-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=44622911&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 44622911,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000372874.9",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.698C>T",
          "hgvs_p": "p.Thr233Ile",
          "transcript": "NM_000022.4",
          "protein_id": "NP_000013.2",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": 790,
          "cdna_end": null,
          "cdna_length": 1496,
          "mane_select": "ENST00000372874.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.698C>T",
          "hgvs_p": "p.Thr233Ile",
          "transcript": "ENST00000372874.9",
          "protein_id": "ENSP00000361965.4",
          "transcript_support_level": 1,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": 790,
          "cdna_end": null,
          "cdna_length": 1496,
          "mane_select": "NM_000022.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.626C>T",
          "hgvs_p": "p.Thr209Ile",
          "transcript": "ENST00000537820.2",
          "protein_id": "ENSP00000441818.1",
          "transcript_support_level": 1,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": 764,
          "cdna_end": null,
          "cdna_length": 2387,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.308C>T",
          "hgvs_p": "p.Thr103Ile",
          "transcript": "ENST00000695995.1",
          "protein_id": "ENSP00000512318.1",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 233,
          "cds_start": 308,
          "cds_end": null,
          "cds_length": 702,
          "cdna_start": 379,
          "cdna_end": null,
          "cdna_length": 1085,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.236C>T",
          "hgvs_p": "p.Thr79Ile",
          "transcript": "ENST00000695991.1",
          "protein_id": "ENSP00000512314.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 209,
          "cds_start": 236,
          "cds_end": null,
          "cds_length": 630,
          "cdna_start": 307,
          "cdna_end": null,
          "cdna_length": 1014,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "n.*520C>T",
          "hgvs_p": null,
          "transcript": "ENST00000696038.1",
          "protein_id": "ENSP00000512344.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1925,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "n.*520C>T",
          "hgvs_p": null,
          "transcript": "ENST00000696038.1",
          "protein_id": "ENSP00000512344.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1925,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.238C>T",
          "hgvs_p": "p.Gln80*",
          "transcript": "ENST00000695889.1",
          "protein_id": "ENSP00000512240.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 238,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": 334,
          "cdna_end": null,
          "cdna_length": 975,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Gln29*",
          "transcript": "ENST00000696065.1",
          "protein_id": "ENSP00000512368.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 137,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 414,
          "cdna_start": 219,
          "cdna_end": null,
          "cdna_length": 726,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.767C>T",
          "hgvs_p": "p.Thr256Ile",
          "transcript": "ENST00000696076.1",
          "protein_id": "ENSP00000512375.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": 875,
          "cdna_end": null,
          "cdna_length": 2032,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.695C>T",
          "hgvs_p": "p.Thr232Ile",
          "transcript": "ENST00000696017.1",
          "protein_id": "ENSP00000512333.1",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 695,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 774,
          "cdna_end": null,
          "cdna_length": 1927,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.698C>T",
          "hgvs_p": "p.Thr233Ile",
          "transcript": "ENST00000696080.1",
          "protein_id": "ENSP00000512379.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": 808,
          "cdna_end": null,
          "cdna_length": 1502,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.695C>T",
          "hgvs_p": "p.Thr232Ile",
          "transcript": "ENST00000696079.1",
          "protein_id": "ENSP00000512378.1",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 695,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 766,
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          "cdna_length": 1464,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.776C>T",
          "hgvs_p": "p.Thr259Ile",
          "transcript": "ENST00000695927.1",
          "protein_id": "ENSP00000512270.1",
          "transcript_support_level": null,
          "aa_start": 259,
          "aa_end": null,
          "aa_length": 389,
          "cds_start": 776,
          "cds_end": null,
          "cds_length": 1170,
          "cdna_start": 940,
          "cdna_end": null,
          "cdna_length": 1630,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.773C>T",
          "hgvs_p": "p.Thr258Ile",
          "transcript": "ENST00000696063.1",
          "protein_id": "ENSP00000512366.1",
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.773C>T",
          "hgvs_p": "p.Thr258Ile",
          "transcript": "ENST00000696082.1",
          "protein_id": "ENSP00000512380.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": 773,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": 804,
          "cdna_end": null,
          "cdna_length": 1479,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.698C>T",
          "hgvs_p": "p.Thr233Ile",
          "transcript": "ENST00000695993.1",
          "protein_id": "ENSP00000512316.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 698,
          "cds_end": null,
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          "cdna_start": 808,
          "cdna_end": null,
          "cdna_length": 1612,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.695C>T",
          "hgvs_p": "p.Thr232Ile",
          "transcript": "ENST00000696058.1",
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.767C>T",
          "hgvs_p": "p.Thr256Ile",
          "transcript": "ENST00000696060.1",
          "protein_id": "ENSP00000512363.1",
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          "cdna_start": 894,
          "cdna_end": null,
          "cdna_length": 1600,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.761C>T",
          "hgvs_p": "p.Thr254Ile",
          "transcript": "ENST00000696062.1",
          "protein_id": "ENSP00000512365.1",
          "transcript_support_level": null,
          "aa_start": 254,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 761,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": 781,
          "cdna_end": null,
          "cdna_length": 1487,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ADA",
          "gene_hgnc_id": 186,
          "hgvs_c": "c.695C>T",
          "hgvs_p": "p.Thr232Ile",
          "transcript": "ENST00000696061.1",
          "protein_id": "ENSP00000512364.1",
          "transcript_support_level": null,
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          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 848,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ADA",
      "gene_hgnc_id": 186,
      "dbsnp": "rs121908729",
      "frequency_reference_population": 6.840441e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84044e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9832590222358704,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.756,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.6158,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.27,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.257,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM1",
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000372874.9",
          "gene_symbol": "ADA",
          "hgnc_id": 186,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.698C>T",
          "hgvs_p": "p.Thr233Ile"
        },
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000372887.5",
          "gene_symbol": "PKIG",
          "hgnc_id": 9019,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.152-1022G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " B cell-negative, NK cell-negative, T cell-negative, autosomal recessive, due to adenosine deaminase deficiency,Partial adenosine deaminase deficiency,Severe combined immunodeficiency",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1 O:1",
      "phenotype_combined": "Partial adenosine deaminase deficiency|Severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-negative, due to adenosine deaminase deficiency",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}