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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-45891714-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=45891714&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 45891714,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_000308.4",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "NM_000308.4",
          "protein_id": "NP_000299.3",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000646241.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000308.4"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000646241.3",
          "protein_id": "ENSP00000493613.2",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000308.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000646241.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.200A>G",
          "hgvs_p": "p.Gln67Arg",
          "transcript": "ENST00000372484.8",
          "protein_id": "ENSP00000361562.3",
          "transcript_support_level": 1,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 200,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000372484.8"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000191018.9",
          "protein_id": "ENSP00000191018.5",
          "transcript_support_level": 1,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000191018.9"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000372459.7",
          "protein_id": "ENSP00000361537.2",
          "transcript_support_level": 1,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000372459.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.196A>G",
          "hgvs_p": null,
          "transcript": "ENST00000484855.4",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000484855.4"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000958428.1",
          "protein_id": "ENSP00000628487.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958428.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000867522.1",
          "protein_id": "ENSP00000537581.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1560,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867522.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.200A>G",
          "hgvs_p": "p.Gln67Arg",
          "transcript": "ENST00000677394.1",
          "protein_id": "ENSP00000504790.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 200,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677394.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "NM_001127695.3",
          "protein_id": "NP_001121167.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001127695.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000607482.6",
          "protein_id": "ENSP00000475524.2",
          "transcript_support_level": 3,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000607482.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000867515.1",
          "protein_id": "ENSP00000537574.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867515.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000867518.1",
          "protein_id": "ENSP00000537577.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867518.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000958426.1",
          "protein_id": "ENSP00000628485.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958426.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000867516.1",
          "protein_id": "ENSP00000537575.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867516.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000958422.1",
          "protein_id": "ENSP00000628481.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958422.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000958423.1",
          "protein_id": "ENSP00000628482.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958423.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000867514.1",
          "protein_id": "ENSP00000537573.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867514.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000867517.1",
          "protein_id": "ENSP00000537576.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867517.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg",
          "transcript": "ENST00000958432.1",
          "protein_id": "ENSP00000628491.1",
          "transcript_support_level": null,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958432.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
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          "hgvs_p": null,
          "transcript": "ENST00000606782.3",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000606782.3"
        }
      ],
      "gene_symbol": "CTSA",
      "gene_hgnc_id": 9251,
      "dbsnp": "rs137854541",
      "frequency_reference_population": 0.0000037188731,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 6,
      "gnomad_exomes_af": 0.00000273746,
      "gnomad_genomes_af": 0.000013142,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9776525497436523,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.6499999761581421,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.87,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8637,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.34,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.86,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.65,
      "spliceai_max_prediction": "Pathogenic",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PP3,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_000308.4",
          "gene_symbol": "CTSA",
          "hgnc_id": 9251,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.146A>G",
          "hgvs_p": "p.Gln49Arg"
        }
      ],
      "clinvar_disease": "Combined deficiency of sialidase AND beta galactosidase",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:1",
      "phenotype_combined": "Combined deficiency of sialidase AND beta galactosidase",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}