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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-46560194-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=46560194&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 46560194,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000279027.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1637G>A",
          "hgvs_p": "p.Arg546Gln",
          "transcript": "NM_022829.6",
          "protein_id": "NP_073740.2",
          "transcript_support_level": null,
          "aa_start": 546,
          "aa_end": null,
          "aa_length": 602,
          "cds_start": 1637,
          "cds_end": null,
          "cds_length": 1809,
          "cdna_start": 1675,
          "cdna_end": null,
          "cdna_length": 4041,
          "mane_select": "ENST00000279027.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1637G>A",
          "hgvs_p": "p.Arg546Gln",
          "transcript": "ENST00000279027.9",
          "protein_id": "ENSP00000279027.4",
          "transcript_support_level": 1,
          "aa_start": 546,
          "aa_end": null,
          "aa_length": 602,
          "cds_start": 1637,
          "cds_end": null,
          "cds_length": 1809,
          "cdna_start": 1675,
          "cdna_end": null,
          "cdna_length": 4041,
          "mane_select": "NM_022829.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1496G>A",
          "hgvs_p": "p.Arg499Gln",
          "transcript": "ENST00000495082.5",
          "protein_id": "ENSP00000419621.1",
          "transcript_support_level": 1,
          "aa_start": 499,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 1496,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": 1687,
          "cdna_end": null,
          "cdna_length": 1887,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1496G>A",
          "hgvs_p": "p.Arg499Gln",
          "transcript": "NM_001011554.3",
          "protein_id": "NP_001011554.1",
          "transcript_support_level": null,
          "aa_start": 499,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 1496,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": 1713,
          "cdna_end": null,
          "cdna_length": 4079,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1496G>A",
          "hgvs_p": "p.Arg499Gln",
          "transcript": "ENST00000290317.9",
          "protein_id": "ENSP00000290317.5",
          "transcript_support_level": 5,
          "aa_start": 499,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 1496,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": 1623,
          "cdna_end": null,
          "cdna_length": 3994,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1487G>A",
          "hgvs_p": "p.Arg496Gln",
          "transcript": "NM_001193339.2",
          "protein_id": "NP_001180268.1",
          "transcript_support_level": null,
          "aa_start": 496,
          "aa_end": null,
          "aa_length": 552,
          "cds_start": 1487,
          "cds_end": null,
          "cds_length": 1659,
          "cdna_start": 1525,
          "cdna_end": null,
          "cdna_length": 3891,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1487G>A",
          "hgvs_p": "p.Arg496Gln",
          "transcript": "ENST00000413164.6",
          "protein_id": "ENSP00000415852.2",
          "transcript_support_level": 2,
          "aa_start": 496,
          "aa_end": null,
          "aa_length": 552,
          "cds_start": 1487,
          "cds_end": null,
          "cds_length": 1659,
          "cdna_start": 1525,
          "cdna_end": null,
          "cdna_length": 2008,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1391G>A",
          "hgvs_p": "p.Arg464Gln",
          "transcript": "NM_001193340.2",
          "protein_id": "NP_001180269.1",
          "transcript_support_level": null,
          "aa_start": 464,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 1391,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": 1608,
          "cdna_end": null,
          "cdna_length": 3974,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1391G>A",
          "hgvs_p": "p.Arg464Gln",
          "transcript": "ENST00000472148.5",
          "protein_id": "ENSP00000420177.1",
          "transcript_support_level": 5,
          "aa_start": 464,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 1391,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": 1518,
          "cdna_end": null,
          "cdna_length": 3283,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC13A3",
          "gene_hgnc_id": 14430,
          "hgvs_c": "c.1343G>A",
          "hgvs_p": "p.Arg448Gln",
          "transcript": "NM_001193342.2",
          "protein_id": "NP_001180271.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 1343,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": 1502,
          "cdna_end": null,
          "cdna_length": 3868,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SLC13A3",
      "gene_hgnc_id": 14430,
      "dbsnp": "rs188700676",
      "frequency_reference_population": 0.00016914394,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 273,
      "gnomad_exomes_af": 0.00017171,
      "gnomad_genomes_af": 0.000144505,
      "gnomad_exomes_ac": 251,
      "gnomad_genomes_ac": 22,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.03153678774833679,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.252,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1101,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.2,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.976,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000279027.9",
          "gene_symbol": "SLC13A3",
          "hgnc_id": 14430,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.1637G>A",
          "hgvs_p": "p.Arg546Gln"
        }
      ],
      "clinvar_disease": " acute reversible, with increased urinary alpha-ketoglutarate,Leukoencephalopathy,Prostate cancer,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Prostate cancer|not provided|Leukoencephalopathy, acute reversible, with increased urinary alpha-ketoglutarate",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}