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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-50935152-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=50935152&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 50935152,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001317034.1",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.763A>G",
          "hgvs_p": "p.Thr255Ala",
          "transcript": "NM_003859.3",
          "protein_id": "NP_003850.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000371588.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003859.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.763A>G",
          "hgvs_p": "p.Thr255Ala",
          "transcript": "ENST00000371588.10",
          "protein_id": "ENSP00000360644.5",
          "transcript_support_level": 1,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003859.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371588.10"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.844A>G",
          "hgvs_p": "p.Thr282Ala",
          "transcript": "ENST00000371582.8",
          "protein_id": "ENSP00000360638.4",
          "transcript_support_level": 1,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 844,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371582.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "n.*218A>G",
          "hgvs_p": null,
          "transcript": "ENST00000466152.5",
          "protein_id": "ENSP00000507119.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000466152.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "n.*218A>G",
          "hgvs_p": null,
          "transcript": "ENST00000466152.5",
          "protein_id": "ENSP00000507119.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000466152.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.868A>G",
          "hgvs_p": "p.Thr290Ala",
          "transcript": "NM_001317034.1",
          "protein_id": "NP_001303963.1",
          "transcript_support_level": null,
          "aa_start": 290,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": 868,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317034.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.868A>G",
          "hgvs_p": "p.Thr290Ala",
          "transcript": "ENST00000371584.9",
          "protein_id": "ENSP00000360640.5",
          "transcript_support_level": 5,
          "aa_start": 290,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": 868,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371584.9"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.844A>G",
          "hgvs_p": "p.Thr282Ala",
          "transcript": "NM_001317035.1",
          "protein_id": "NP_001303964.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 844,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317035.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.760A>G",
          "hgvs_p": "p.Thr254Ala",
          "transcript": "ENST00000970159.1",
          "protein_id": "ENSP00000640218.1",
          "transcript_support_level": null,
          "aa_start": 254,
          "aa_end": null,
          "aa_length": 259,
          "cds_start": 760,
          "cds_end": null,
          "cds_length": 780,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970159.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.727A>G",
          "hgvs_p": "p.Thr243Ala",
          "transcript": "ENST00000922811.1",
          "protein_id": "ENSP00000592870.1",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": 727,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922811.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.694A>G",
          "hgvs_p": "p.Thr232Ala",
          "transcript": "NM_001317036.1",
          "protein_id": "NP_001303965.1",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 237,
          "cds_start": 694,
          "cds_end": null,
          "cds_length": 714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317036.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.667A>G",
          "hgvs_p": "p.Thr223Ala",
          "transcript": "ENST00000858753.1",
          "protein_id": "ENSP00000528812.1",
          "transcript_support_level": null,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858753.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.598A>G",
          "hgvs_p": "p.Thr200Ala",
          "transcript": "ENST00000858754.1",
          "protein_id": "ENSP00000528813.1",
          "transcript_support_level": null,
          "aa_start": 200,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 598,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858754.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.430A>G",
          "hgvs_p": "p.Thr144Ala",
          "transcript": "ENST00000922813.1",
          "protein_id": "ENSP00000592872.1",
          "transcript_support_level": null,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 149,
          "cds_start": 430,
          "cds_end": null,
          "cds_length": 450,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922813.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.379A>G",
          "hgvs_p": "p.Thr127Ala",
          "transcript": "ENST00000683466.1",
          "protein_id": "ENSP00000507404.1",
          "transcript_support_level": null,
          "aa_start": 127,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 379,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000683466.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "c.361A>G",
          "hgvs_p": "p.Thr121Ala",
          "transcript": "ENST00000922812.1",
          "protein_id": "ENSP00000592871.1",
          "transcript_support_level": null,
          "aa_start": 121,
          "aa_end": null,
          "aa_length": 126,
          "cds_start": 361,
          "cds_end": null,
          "cds_length": 381,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922812.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "n.533A>G",
          "hgvs_p": null,
          "transcript": "ENST00000494752.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000494752.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "n.2019A>G",
          "hgvs_p": null,
          "transcript": "ENST00000682366.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000682366.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "n.1243A>G",
          "hgvs_p": null,
          "transcript": "ENST00000682713.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000682713.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPM1",
          "gene_hgnc_id": 3005,
          "hgvs_c": "n.*278A>G",
          "hgvs_p": null,
          "transcript": "ENST00000683048.1",
          "protein_id": "ENSP00000506986.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000683048.1"
        },
        {
          "aa_ref": null,
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      "computational_score_selected": 0.2366616427898407,
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      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.13,
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      "phylop100way_score": 5.763,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
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      "acmg_score": 0,
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      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
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            "BP4_Moderate"
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          "verdict": "Uncertain_significance",
          "transcript": "NM_001317034.1",
          "gene_symbol": "DPM1",
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          "effects": [
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        {
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            "BP4_Moderate"
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          "verdict": "Uncertain_significance",
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          "effects": [
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          "inheritance_mode": "",
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      "clinvar_disease": "Congenital disorder of glycosylation type 1E,Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Congenital disorder of glycosylation type 1E|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
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  ],
  "message": null
}