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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-5106258-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=5106258&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 5106258,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000202834.12",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000612323.4",
          "protein_id": "ENSP00000478641.1",
          "transcript_support_level": 1,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": 294,
          "cdna_end": null,
          "cdna_length": 1231,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000202834.12",
          "protein_id": "ENSP00000202834.7",
          "transcript_support_level": 1,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 283,
          "cdna_end": null,
          "cdna_length": 1435,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000379299.6",
          "protein_id": "ENSP00000368601.2",
          "transcript_support_level": 1,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 480,
          "cdna_end": null,
          "cdna_length": 1642,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001330987.2",
          "protein_id": "NP_001317916.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": 283,
          "cdna_end": null,
          "cdna_length": 1328,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000615008.4",
          "protein_id": "ENSP00000478112.1",
          "transcript_support_level": 4,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": 333,
          "cdna_end": null,
          "cdna_length": 594,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001009924.2",
          "protein_id": "NP_001009924.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 607,
          "cdna_end": null,
          "cdna_length": 1759,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001009925.2",
          "protein_id": "NP_001009925.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 283,
          "cdna_end": null,
          "cdna_length": 1435,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001330984.2",
          "protein_id": "NP_001317913.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 559,
          "cdna_end": null,
          "cdna_length": 1711,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001330985.2",
          "protein_id": "NP_001317914.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 1676,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001330986.2",
          "protein_id": "NP_001317915.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 501,
          "cdna_end": null,
          "cdna_length": 1653,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
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          "strand": false,
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          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_001423980.1",
          "protein_id": "NP_001410909.1",
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          "aa_start": 51,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
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          "cdna_start": 389,
          "cdna_end": null,
          "cdna_length": 1541,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
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          "protein_coding": true,
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          "consequences": [
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          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "NM_014145.5",
          "protein_id": "NP_054864.3",
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        },
        {
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          "intron_rank": null,
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          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
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        {
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          "intron_rank": null,
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          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
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          "protein_id": "ENSP00000368579.2",
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          "biotype": null,
          "feature": null
        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "TMEM230",
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          "feature": null
        },
        {
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          "strand": false,
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000379283.6",
          "protein_id": "ENSP00000368585.2",
          "transcript_support_level": 3,
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          "aa_end": null,
          "aa_length": 120,
          "cds_start": 152,
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          "cdna_start": 498,
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          "cdna_length": 1660,
          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000379286.6",
          "protein_id": "ENSP00000368588.2",
          "transcript_support_level": 2,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala",
          "transcript": "ENST00000379276.4",
          "protein_id": "ENSP00000368578.4",
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          "feature": null
        },
        {
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "n.568T>C",
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          "transcript": "ENST00000492419.1",
          "protein_id": null,
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          "mane_select": null,
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        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "n.389T>C",
          "hgvs_p": null,
          "transcript": "XR_002958476.2",
          "protein_id": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 1371,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "n.283T>C",
          "hgvs_p": null,
          "transcript": "XR_002958478.2",
          "protein_id": null,
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          "aa_length": null,
          "cds_start": -4,
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          "exon_count": 6,
          "intron_rank": null,
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        },
        {
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          "protein_coding": false,
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          "gene_symbol": "TMEM230",
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          "hgvs_c": "n.559T>C",
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          "transcript": "XR_002958484.2",
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        },
        {
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          "protein_coding": false,
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          "consequences": [
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          "exon_rank": 4,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "n.389T>C",
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          "transcript": "XR_007067454.1",
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          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": 1372,
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        },
        {
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          "strand": false,
          "consequences": [
            "intron_variant"
          ],
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          "exon_count": 4,
          "intron_rank": 3,
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          "gene_symbol": "TMEM230",
          "gene_hgnc_id": 15876,
          "hgvs_c": "c.288+3074T>C",
          "hgvs_p": null,
          "transcript": "XM_047440132.1",
          "protein_id": "XP_047296088.1",
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          "aa_length": 142,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 429,
          "cdna_start": null,
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          "cdna_length": 1418,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "TMEM230",
      "gene_hgnc_id": 15876,
      "dbsnp": "rs193920937",
      "frequency_reference_population": 0.000001368135,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136814,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5656325817108154,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.214,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1965,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.19,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 8.511,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000202834.12",
          "gene_symbol": "TMEM230",
          "hgnc_id": 15876,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "SD,AD",
          "hgvs_c": "c.152T>C",
          "hgvs_p": "p.Val51Ala"
        }
      ],
      "clinvar_disease": "Prostate cancer",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Prostate cancer",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}