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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-57374724-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=57374724&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 57374724,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_003610.4",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "NM_003610.4",
          "protein_id": "NP_003601.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000395841.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003610.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "ENST00000395841.7",
          "protein_id": "ENSP00000379182.2",
          "transcript_support_level": 1,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003610.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395841.7"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "ENST00000527947.5",
          "protein_id": "ENSP00000432609.1",
          "transcript_support_level": 1,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 437,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1314,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000527947.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "ENST00000395840.6",
          "protein_id": "ENSP00000379181.2",
          "transcript_support_level": 1,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395840.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "NM_001015885.2",
          "protein_id": "NP_001015885.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001015885.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "ENST00000371242.6",
          "protein_id": "ENSP00000360286.2",
          "transcript_support_level": 5,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371242.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "ENST00000857607.1",
          "protein_id": "ENSP00000527666.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857607.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.871G>C",
          "hgvs_p": "p.Asp291His",
          "transcript": "ENST00000922550.1",
          "protein_id": "ENSP00000592609.1",
          "transcript_support_level": null,
          "aa_start": 291,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": 871,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922550.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.856G>C",
          "hgvs_p": "p.Asp286His",
          "transcript": "ENST00000947295.1",
          "protein_id": "ENSP00000617354.1",
          "transcript_support_level": null,
          "aa_start": 286,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": 856,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000947295.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.835G>C",
          "hgvs_p": "p.Asp279His",
          "transcript": "ENST00000947294.1",
          "protein_id": "ENSP00000617353.1",
          "transcript_support_level": null,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 835,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000947294.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "XM_005260582.3",
          "protein_id": "XP_005260639.2",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005260582.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "XM_005260583.3",
          "protein_id": "XP_005260640.2",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005260583.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "XM_011529088.3",
          "protein_id": "XP_011527390.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529088.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "XM_011529089.2",
          "protein_id": "XP_011527391.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529089.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His",
          "transcript": "XM_011529087.3",
          "protein_id": "XP_011527389.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 357,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1074,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529087.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "c.825+986G>C",
          "hgvs_p": null,
          "transcript": "ENST00000922549.1",
          "protein_id": "ENSP00000592608.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 303,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922549.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "n.2723G>C",
          "hgvs_p": null,
          "transcript": "ENST00000462438.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000462438.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "n.*182G>C",
          "hgvs_p": null,
          "transcript": "ENST00000492498.5",
          "protein_id": "ENSP00000432698.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000492498.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAE1",
          "gene_hgnc_id": 9828,
          "hgvs_c": "n.*182G>C",
          "hgvs_p": null,
          "transcript": "ENST00000492498.5",
          "protein_id": "ENSP00000432698.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000492498.5"
        }
      ],
      "gene_symbol": "RAE1",
      "gene_hgnc_id": 9828,
      "dbsnp": "rs933510491",
      "frequency_reference_population": 0.0000055758005,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000547236,
      "gnomad_genomes_af": 0.00000656918,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7709456086158752,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.408,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.8128,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.15,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.579,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3,BS2",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 4,
          "pathogenic_score": 1,
          "criteria": [
            "PP3",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_003610.4",
          "gene_symbol": "RAE1",
          "hgnc_id": 9828,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.943G>C",
          "hgvs_p": "p.Asp315His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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