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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-58909991-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=58909991&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 58909991,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "NM_080425.4",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.2809C>T",
          "hgvs_p": "p.Gln937*",
          "transcript": "NM_080425.4",
          "protein_id": "NP_536350.2",
          "transcript_support_level": null,
          "aa_start": 937,
          "aa_end": null,
          "aa_length": 1037,
          "cds_start": 2809,
          "cds_end": null,
          "cds_length": 3114,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": "ENST00000371100.9",
          "biotype": "protein_coding",
          "feature": "NM_080425.4"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.2809C>T",
          "hgvs_p": "p.Gln937*",
          "transcript": "ENST00000371100.9",
          "protein_id": "ENSP00000360141.3",
          "transcript_support_level": 5,
          "aa_start": 937,
          "aa_end": null,
          "aa_length": 1037,
          "cds_start": 2809,
          "cds_end": null,
          "cds_length": 3114,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": "NM_080425.4",
          "biotype": "protein_coding",
          "feature": "ENST00000371100.9"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.880C>T",
          "hgvs_p": "p.Gln294*",
          "transcript": "NM_000516.7",
          "protein_id": "NP_000507.1",
          "transcript_support_level": null,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 394,
          "cds_start": 880,
          "cds_end": null,
          "cds_length": 1185,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000371085.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000516.7"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.880C>T",
          "hgvs_p": "p.Gln294*",
          "transcript": "ENST00000371085.8",
          "protein_id": "ENSP00000360126.3",
          "transcript_support_level": 1,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 394,
          "cds_start": 880,
          "cds_end": null,
          "cds_length": 1185,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000516.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371085.8"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.2812C>T",
          "hgvs_p": "p.Gln938*",
          "transcript": "ENST00000676826.2",
          "protein_id": "ENSP00000504675.2",
          "transcript_support_level": null,
          "aa_start": 938,
          "aa_end": null,
          "aa_length": 1038,
          "cds_start": 2812,
          "cds_end": null,
          "cds_length": 3117,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676826.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.2767C>T",
          "hgvs_p": "p.Gln923*",
          "transcript": "ENST00000371102.8",
          "protein_id": "ENSP00000360143.4",
          "transcript_support_level": 5,
          "aa_start": 923,
          "aa_end": null,
          "aa_length": 1023,
          "cds_start": 2767,
          "cds_end": null,
          "cds_length": 3072,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371102.8"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.883C>T",
          "hgvs_p": "p.Gln295*",
          "transcript": "ENST00000354359.12",
          "protein_id": "ENSP00000346328.7",
          "transcript_support_level": 1,
          "aa_start": 295,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 883,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000354359.12"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.838C>T",
          "hgvs_p": "p.Gln280*",
          "transcript": "ENST00000371095.7",
          "protein_id": "ENSP00000360136.3",
          "transcript_support_level": 1,
          "aa_start": 280,
          "aa_end": null,
          "aa_length": 380,
          "cds_start": 838,
          "cds_end": null,
          "cds_length": 1143,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371095.7"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.835C>T",
          "hgvs_p": "p.Gln279*",
          "transcript": "ENST00000265620.11",
          "protein_id": "ENSP00000265620.7",
          "transcript_support_level": 1,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": 835,
          "cds_end": null,
          "cds_length": 1140,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265620.11"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.706C>T",
          "hgvs_p": "p.Gln236*",
          "transcript": "ENST00000470512.6",
          "protein_id": "ENSP00000499552.2",
          "transcript_support_level": 5,
          "aa_start": 236,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 706,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000470512.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.706C>T",
          "hgvs_p": "p.Gln236*",
          "transcript": "ENST00000480232.6",
          "protein_id": "ENSP00000499545.2",
          "transcript_support_level": 5,
          "aa_start": 236,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 706,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000480232.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.706C>T",
          "hgvs_p": "p.Gln236*",
          "transcript": "ENST00000663479.2",
          "protein_id": "ENSP00000499353.2",
          "transcript_support_level": null,
          "aa_start": 236,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 706,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000663479.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.703C>T",
          "hgvs_p": "p.Gln235*",
          "transcript": "ENST00000477931.5",
          "protein_id": "ENSP00000499660.1",
          "transcript_support_level": 1,
          "aa_start": 235,
          "aa_end": null,
          "aa_length": 335,
          "cds_start": 703,
          "cds_end": null,
          "cds_length": 1008,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000477931.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000462499.6",
          "protein_id": "ENSP00000499758.2",
          "transcript_support_level": 2,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000462499.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000467227.6",
          "protein_id": "ENSP00000499681.2",
          "transcript_support_level": 3,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000467227.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000478585.6",
          "protein_id": "ENSP00000499762.2",
          "transcript_support_level": 2,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000478585.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000481039.6",
          "protein_id": "ENSP00000499767.2",
          "transcript_support_level": 5,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000481039.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000485673.6",
          "protein_id": "ENSP00000499334.2",
          "transcript_support_level": 5,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000485673.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000488546.6",
          "protein_id": "ENSP00000499332.2",
          "transcript_support_level": 5,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000488546.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GNAS",
          "gene_hgnc_id": 4392,
          "hgvs_c": "c.661C>T",
          "hgvs_p": "p.Gln221*",
          "transcript": "ENST00000492907.6",
          "protein_id": "ENSP00000499443.2",
          "transcript_support_level": 3,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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      "dbsnp": "rs863224876",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6200000047683716,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.05999999865889549,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.62,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.405,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.06,
      "spliceai_max_prediction": "Benign",
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      "apogee2_score": null,
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      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Moderate",
      "acmg_by_gene": [
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          "benign_score": 0,
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            "PP5_Moderate"
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          "verdict": "Pathogenic",
          "transcript": "NM_080425.4",
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          "effects": [
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          "inheritance_mode": "AD,Mitochondrial,Unknown",
          "hgvs_c": "c.2809C>T",
          "hgvs_p": "p.Gln937*"
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      ],
      "clinvar_disease": "Pseudohypoparathyroidism,Pseudohypoparathyroidism type 1C",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Pseudohypoparathyroidism;Pseudohypoparathyroidism type 1C",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}