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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-64083963-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=64083963&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 64083963,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001007125.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "NM_001353425.2",
          "protein_id": "NP_001340354.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000302096.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353425.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "ENST00000302096.5",
          "protein_id": "ENSP00000302763.4",
          "transcript_support_level": 2,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001353425.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000302096.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "ENST00000308906.6",
          "protein_id": "ENSP00000310801.2",
          "transcript_support_level": 1,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000308906.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-185+3611G>A",
          "hgvs_p": null,
          "transcript": "NM_182647.4",
          "protein_id": "NP_872588.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000336866.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_182647.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-185+3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000336866.7",
          "protein_id": "ENSP00000336843.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_182647.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000336866.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-34+3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000355631.8",
          "protein_id": "ENSP00000347848.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355631.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "NM_001007125.3",
          "protein_id": "NP_001007126.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001007125.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "NM_001386971.1",
          "protein_id": "NP_001373900.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001386971.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "ENST00000879466.1",
          "protein_id": "ENSP00000549525.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879466.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "XM_024451849.2",
          "protein_id": "XP_024307617.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024451849.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "XM_024451850.2",
          "protein_id": "XP_024307618.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024451850.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LKAAEAR1",
          "gene_hgnc_id": 33718,
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu",
          "transcript": "XM_047439963.1",
          "protein_id": "XP_047295919.1",
          "transcript_support_level": null,
          "aa_start": 86,
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          "aa_length": 194,
          "cds_start": 257,
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          "cds_length": 585,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
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          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-185+3611G>A",
          "hgvs_p": null,
          "transcript": "NM_001318853.2",
          "protein_id": "NP_001305782.1",
          "transcript_support_level": null,
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          "aa_length": 399,
          "cds_start": null,
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          "cds_length": 1200,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001318853.2"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-185+3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000672146.3",
          "protein_id": "ENSP00000500894.2",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": null,
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          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-34+3611G>A",
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          "transcript": "NM_000913.6",
          "protein_id": "NP_000904.1",
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          "cds_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": null,
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-300+3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000879249.1",
          "protein_id": "ENSP00000549308.1",
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        {
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          "intron_rank": 1,
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          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-254+3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000921128.1",
          "protein_id": "ENSP00000591187.1",
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        {
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          "gene_symbol": "OPRL1",
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        {
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          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-34+3611G>A",
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        },
        {
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          "protein_coding": true,
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          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-185+3611G>A",
          "hgvs_p": null,
          "transcript": "XM_047440155.1",
          "protein_id": "XP_047296111.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 365,
          "cds_start": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-34+3611G>A",
          "hgvs_p": null,
          "transcript": "XM_047440156.1",
          "protein_id": "XP_047296112.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440156.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "OPRL1",
          "gene_hgnc_id": 8155,
          "hgvs_c": "c.-11+3611G>A",
          "hgvs_p": null,
          "transcript": "XM_047440157.1",
          "protein_id": "XP_047296113.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 289,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 870,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440157.1"
        }
      ],
      "gene_symbol": "LKAAEAR1",
      "gene_hgnc_id": 33718,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0317130982875824,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.036,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0722,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.74,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.841,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP6_Moderate",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001007125.3",
          "gene_symbol": "LKAAEAR1",
          "hgnc_id": 33718,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.257C>T",
          "hgvs_p": "p.Pro86Leu"
        },
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001318853.2",
          "gene_symbol": "OPRL1",
          "hgnc_id": 8155,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.-185+3611G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}