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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-14500647-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=14500647&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 14500647,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001395858.1",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.650T>C",
          "hgvs_p": "p.Ile217Thr",
          "transcript": "NM_022136.5",
          "protein_id": "NP_071419.3",
          "transcript_support_level": null,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 650,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000400566.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_022136.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.650T>C",
          "hgvs_p": "p.Ile217Thr",
          "transcript": "ENST00000400566.6",
          "protein_id": "ENSP00000383411.2",
          "transcript_support_level": 1,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 650,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_022136.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400566.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.854T>C",
          "hgvs_p": "p.Ile285Thr",
          "transcript": "ENST00000285670.7",
          "protein_id": "ENSP00000285670.2",
          "transcript_support_level": 1,
          "aa_start": 285,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 854,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000285670.7"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.443T>C",
          "hgvs_p": "p.Ile148Thr",
          "transcript": "ENST00000619120.4",
          "protein_id": "ENSP00000480850.1",
          "transcript_support_level": 1,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": 443,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000619120.4"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.146T>C",
          "hgvs_p": "p.Ile49Thr",
          "transcript": "ENST00000400564.5",
          "protein_id": "ENSP00000383409.1",
          "transcript_support_level": 1,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400564.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.1634T>C",
          "hgvs_p": "p.Ile545Thr",
          "transcript": "NM_001395858.1",
          "protein_id": "NP_001382787.1",
          "transcript_support_level": null,
          "aa_start": 545,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1634,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001395858.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.1538T>C",
          "hgvs_p": "p.Ile513Thr",
          "transcript": "NM_001395857.1",
          "protein_id": "NP_001382786.1",
          "transcript_support_level": null,
          "aa_start": 513,
          "aa_end": null,
          "aa_length": 669,
          "cds_start": 1538,
          "cds_end": null,
          "cds_length": 2010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001395857.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.1538T>C",
          "hgvs_p": "p.Ile513Thr",
          "transcript": "ENST00000647101.1",
          "protein_id": "ENSP00000493867.1",
          "transcript_support_level": null,
          "aa_start": 513,
          "aa_end": null,
          "aa_length": 669,
          "cds_start": 1538,
          "cds_end": null,
          "cds_length": 2010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000647101.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.854T>C",
          "hgvs_p": "p.Ile285Thr",
          "transcript": "NM_001256370.2",
          "protein_id": "NP_001243299.1",
          "transcript_support_level": null,
          "aa_start": 285,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 854,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256370.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.683T>C",
          "hgvs_p": "p.Ile228Thr",
          "transcript": "ENST00000959353.1",
          "protein_id": "ENSP00000629412.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 683,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959353.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.500T>C",
          "hgvs_p": "p.Ile167Thr",
          "transcript": "ENST00000871911.1",
          "protein_id": "ENSP00000541970.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 323,
          "cds_start": 500,
          "cds_end": null,
          "cds_length": 972,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871911.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.443T>C",
          "hgvs_p": "p.Ile148Thr",
          "transcript": "NM_001286523.2",
          "protein_id": "NP_001273452.1",
          "transcript_support_level": null,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": 443,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286523.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.596T>C",
          "hgvs_p": "p.Ile199Thr",
          "transcript": "XM_011529684.3",
          "protein_id": "XP_011527986.1",
          "transcript_support_level": null,
          "aa_start": 199,
          "aa_end": null,
          "aa_length": 355,
          "cds_start": 596,
          "cds_end": null,
          "cds_length": 1068,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529684.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.500T>C",
          "hgvs_p": "p.Ile167Thr",
          "transcript": "XM_011529685.1",
          "protein_id": "XP_011527987.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 323,
          "cds_start": 500,
          "cds_end": null,
          "cds_length": 972,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529685.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.650T>C",
          "hgvs_p": "p.Ile217Thr",
          "transcript": "XM_011529686.2",
          "protein_id": "XP_011527988.1",
          "transcript_support_level": null,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 309,
          "cds_start": 650,
          "cds_end": null,
          "cds_length": 930,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529686.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "c.446T>C",
          "hgvs_p": "p.Ile149Thr",
          "transcript": "XM_047440942.1",
          "protein_id": "XP_047296898.1",
          "transcript_support_level": null,
          "aa_start": 149,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 446,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440942.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "n.1029T>C",
          "hgvs_p": null,
          "transcript": "ENST00000644288.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000644288.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "LOC124905053",
          "gene_hgnc_id": null,
          "hgvs_c": "n.339+22980A>G",
          "hgvs_p": null,
          "transcript": "XR_007067925.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007067925.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SAMSN1",
          "gene_hgnc_id": 10528,
          "hgvs_c": "n.-41T>C",
          "hgvs_p": null,
          "transcript": "ENST00000463807.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000463807.1"
        }
      ],
      "gene_symbol": "SAMSN1",
      "gene_hgnc_id": 10528,
      "dbsnp": "rs373185460",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7905018329620361,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.507,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9835,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.12,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 8.947,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001395858.1",
          "gene_symbol": "SAMSN1",
          "hgnc_id": 10528,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1634T>C",
          "hgvs_p": "p.Ile545Thr"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_007067925.1",
          "gene_symbol": "LOC124905053",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.339+22980A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}