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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-18260215-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=18260215&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 18260215,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_024944.3",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.563C>T",
          "hgvs_p": "p.Ala188Val",
          "transcript": "NM_024944.3",
          "protein_id": "NP_079220.2",
          "transcript_support_level": null,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000299295.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024944.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.563C>T",
          "hgvs_p": "p.Ala188Val",
          "transcript": "ENST00000299295.7",
          "protein_id": "ENSP00000299295.2",
          "transcript_support_level": 1,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024944.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000299295.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "ENST00000400131.5",
          "protein_id": "ENSP00000382996.1",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400131.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "ENST00000400135.5",
          "protein_id": "ENSP00000383001.1",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400135.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "ENST00000400127.5",
          "protein_id": "ENSP00000382992.1",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400127.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.506C>T",
          "hgvs_p": "p.Ala169Val",
          "transcript": "NM_001204174.2",
          "protein_id": "NP_001191103.1",
          "transcript_support_level": null,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 254,
          "cds_start": 506,
          "cds_end": null,
          "cds_length": 765,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204174.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.506C>T",
          "hgvs_p": "p.Ala169Val",
          "transcript": "ENST00000543733.5",
          "protein_id": "ENSP00000443566.1",
          "transcript_support_level": 2,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 254,
          "cds_start": 506,
          "cds_end": null,
          "cds_length": 765,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543733.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "NM_001204177.2",
          "protein_id": "NP_001191106.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204177.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "NM_001204178.2",
          "protein_id": "NP_001191107.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204178.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "ENST00000338326.4",
          "protein_id": "ENSP00000339975.3",
          "transcript_support_level": 5,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000338326.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "NM_001204175.2",
          "protein_id": "NP_001191104.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204175.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "NM_001204176.2",
          "protein_id": "NP_001191105.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204176.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.440C>T",
          "hgvs_p": "p.Ala147Val",
          "transcript": "ENST00000400128.5",
          "protein_id": "ENSP00000382993.1",
          "transcript_support_level": 2,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400128.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.563C>T",
          "hgvs_p": "p.Ala188Val",
          "transcript": "XM_011529453.3",
          "protein_id": "XP_011527755.1",
          "transcript_support_level": null,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529453.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.530C>T",
          "hgvs_p": "p.Ala177Val",
          "transcript": "XM_047440682.1",
          "protein_id": "XP_047296638.1",
          "transcript_support_level": null,
          "aa_start": 177,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 530,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440682.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CHODL",
          "gene_hgnc_id": 17807,
          "hgvs_c": "c.506C>T",
          "hgvs_p": "p.Ala169Val",
          "transcript": "XM_017028273.2",
          "protein_id": "XP_016883762.1",
          "transcript_support_level": null,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 506,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017028273.2"
        }
      ],
      "gene_symbol": "CHODL",
      "gene_hgnc_id": 17807,
      "dbsnp": "rs1189009907",
      "frequency_reference_population": 0.0000025473034,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000141014,
      "gnomad_genomes_af": 0.0000131584,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.02203655242919922,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.071,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0628,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.83,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.315,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_024944.3",
          "gene_symbol": "CHODL",
          "hgnc_id": 17807,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.563C>T",
          "hgvs_p": "p.Ala188Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}