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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-25592835-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=25592835&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 25592835,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_080794.4",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu",
          "transcript": "NM_017446.4",
          "protein_id": "NP_059142.3",
          "transcript_support_level": null,
          "aa_start": 300,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 898,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000352957.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017446.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu",
          "transcript": "ENST00000352957.9",
          "protein_id": "ENSP00000284967.7",
          "transcript_support_level": 1,
          "aa_start": 300,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 898,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017446.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000352957.9"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu",
          "transcript": "NM_080794.4",
          "protein_id": "NP_542984.3",
          "transcript_support_level": null,
          "aa_start": 300,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 898,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_080794.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu",
          "transcript": "ENST00000307301.11",
          "protein_id": "ENSP00000305682.7",
          "transcript_support_level": 5,
          "aa_start": 300,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 898,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000307301.11"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.916G>T",
          "hgvs_p": "p.Val306Leu",
          "transcript": "ENST00000925346.1",
          "protein_id": "ENSP00000595405.1",
          "transcript_support_level": null,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": 916,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925346.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.901G>T",
          "hgvs_p": "p.Val301Leu",
          "transcript": "ENST00000925341.1",
          "protein_id": "ENSP00000595400.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": 901,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925341.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.895G>T",
          "hgvs_p": "p.Val299Leu",
          "transcript": "ENST00000946900.1",
          "protein_id": "ENSP00000616959.1",
          "transcript_support_level": null,
          "aa_start": 299,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 895,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946900.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.859G>T",
          "hgvs_p": "p.Val287Leu",
          "transcript": "ENST00000925344.1",
          "protein_id": "ENSP00000595403.1",
          "transcript_support_level": null,
          "aa_start": 287,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 859,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925344.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.853G>T",
          "hgvs_p": "p.Val285Leu",
          "transcript": "ENST00000946901.1",
          "protein_id": "ENSP00000616960.1",
          "transcript_support_level": null,
          "aa_start": 285,
          "aa_end": null,
          "aa_length": 323,
          "cds_start": 853,
          "cds_end": null,
          "cds_length": 972,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946901.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu",
          "transcript": "ENST00000925340.1",
          "protein_id": "ENSP00000595399.1",
          "transcript_support_level": null,
          "aa_start": 300,
          "aa_end": null,
          "aa_length": 322,
          "cds_start": 898,
          "cds_end": null,
          "cds_length": 969,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925340.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.847G>T",
          "hgvs_p": "p.Val283Leu",
          "transcript": "ENST00000875588.1",
          "protein_id": "ENSP00000545647.1",
          "transcript_support_level": null,
          "aa_start": 283,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 847,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000875588.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.844G>T",
          "hgvs_p": "p.Val282Leu",
          "transcript": "ENST00000875589.1",
          "protein_id": "ENSP00000545648.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 320,
          "cds_start": 844,
          "cds_end": null,
          "cds_length": 963,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000875589.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.784G>T",
          "hgvs_p": "p.Val262Leu",
          "transcript": "ENST00000925343.1",
          "protein_id": "ENSP00000595402.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925343.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.868G>T",
          "hgvs_p": "p.Val290Leu",
          "transcript": "ENST00000419219.1",
          "protein_id": "ENSP00000404426.1",
          "transcript_support_level": 5,
          "aa_start": 290,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 868,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000419219.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.691G>T",
          "hgvs_p": "p.Val231Leu",
          "transcript": "ENST00000925345.1",
          "protein_id": "ENSP00000595404.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 691,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925345.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.658G>T",
          "hgvs_p": "p.Val220Leu",
          "transcript": "ENST00000925342.1",
          "protein_id": "ENSP00000595401.1",
          "transcript_support_level": null,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 658,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925342.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu",
          "transcript": "XM_006724026.5",
          "protein_id": "XP_006724089.1",
          "transcript_support_level": null,
          "aa_start": 300,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 898,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006724026.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPL39",
          "gene_hgnc_id": 14027,
          "hgvs_c": "c.772G>T",
          "hgvs_p": "p.Val258Leu",
          "transcript": "XM_011529651.3",
          "protein_id": "XP_011527953.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 772,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011529651.3"
        }
      ],
      "gene_symbol": "MRPL39",
      "gene_hgnc_id": 14027,
      "dbsnp": "rs115668266",
      "frequency_reference_population": 0.0003033604,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 489,
      "gnomad_exomes_af": 0.000172641,
      "gnomad_genomes_af": 0.00155647,
      "gnomad_exomes_ac": 252,
      "gnomad_genomes_ac": 237,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.009847462177276611,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.1599999964237213,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.079,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0848,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.5,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.011,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.16,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 4,
          "pathogenic_score": 1,
          "criteria": [
            "PP2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_080794.4",
          "gene_symbol": "MRPL39",
          "hgnc_id": 14027,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.898G>T",
          "hgvs_p": "p.Val300Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}