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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-28931223-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=28931223&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 28931223,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_015565.3",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5170G>A",
          "hgvs_p": "p.Gly1724Ser",
          "transcript": "NM_015565.3",
          "protein_id": "NP_056380.3",
          "transcript_support_level": null,
          "aa_start": 1724,
          "aa_end": null,
          "aa_length": 1766,
          "cds_start": 5170,
          "cds_end": null,
          "cds_length": 5301,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000361371.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015565.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5170G>A",
          "hgvs_p": "p.Gly1724Ser",
          "transcript": "ENST00000361371.10",
          "protein_id": "ENSP00000354977.4",
          "transcript_support_level": 1,
          "aa_start": 1724,
          "aa_end": null,
          "aa_length": 1766,
          "cds_start": 5170,
          "cds_end": null,
          "cds_length": 5301,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015565.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361371.10"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5308G>A",
          "hgvs_p": "p.Gly1770Ser",
          "transcript": "ENST00000614971.4",
          "protein_id": "ENSP00000478783.1",
          "transcript_support_level": 1,
          "aa_start": 1770,
          "aa_end": null,
          "aa_length": 1812,
          "cds_start": 5308,
          "cds_end": null,
          "cds_length": 5439,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000614971.4"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5170G>A",
          "hgvs_p": "p.Gly1724Ser",
          "transcript": "ENST00000389194.7",
          "protein_id": "ENSP00000373846.3",
          "transcript_support_level": 1,
          "aa_start": 1724,
          "aa_end": null,
          "aa_length": 1766,
          "cds_start": 5170,
          "cds_end": null,
          "cds_length": 5301,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000389194.7"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5137G>A",
          "hgvs_p": "p.Gly1713Ser",
          "transcript": "ENST00000912878.1",
          "protein_id": "ENSP00000582937.1",
          "transcript_support_level": null,
          "aa_start": 1713,
          "aa_end": null,
          "aa_length": 1755,
          "cds_start": 5137,
          "cds_end": null,
          "cds_length": 5268,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912878.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5128G>A",
          "hgvs_p": "p.Gly1710Ser",
          "transcript": "NM_001320766.2",
          "protein_id": "NP_001307695.2",
          "transcript_support_level": null,
          "aa_start": 1710,
          "aa_end": null,
          "aa_length": 1752,
          "cds_start": 5128,
          "cds_end": null,
          "cds_length": 5259,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001320766.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.5128G>A",
          "hgvs_p": "p.Gly1710Ser",
          "transcript": "ENST00000912877.1",
          "protein_id": "ENSP00000582936.1",
          "transcript_support_level": null,
          "aa_start": 1710,
          "aa_end": null,
          "aa_length": 1752,
          "cds_start": 5128,
          "cds_end": null,
          "cds_length": 5259,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912877.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.4975G>A",
          "hgvs_p": "p.Gly1659Ser",
          "transcript": "ENST00000881243.1",
          "protein_id": "ENSP00000551302.1",
          "transcript_support_level": null,
          "aa_start": 1659,
          "aa_end": null,
          "aa_length": 1701,
          "cds_start": 4975,
          "cds_end": null,
          "cds_length": 5106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881243.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.4957G>A",
          "hgvs_p": "p.Gly1653Ser",
          "transcript": "ENST00000912879.1",
          "protein_id": "ENSP00000582938.1",
          "transcript_support_level": null,
          "aa_start": 1653,
          "aa_end": null,
          "aa_length": 1695,
          "cds_start": 4957,
          "cds_end": null,
          "cds_length": 5088,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912879.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.4453G>A",
          "hgvs_p": "p.Gly1485Ser",
          "transcript": "XM_047440740.1",
          "protein_id": "XP_047296696.1",
          "transcript_support_level": null,
          "aa_start": 1485,
          "aa_end": null,
          "aa_length": 1527,
          "cds_start": 4453,
          "cds_end": null,
          "cds_length": 4584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440740.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.4303G>A",
          "hgvs_p": "p.Gly1435Ser",
          "transcript": "XM_047440741.1",
          "protein_id": "XP_047296697.1",
          "transcript_support_level": null,
          "aa_start": 1435,
          "aa_end": null,
          "aa_length": 1477,
          "cds_start": 4303,
          "cds_end": null,
          "cds_length": 4434,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440741.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.3037G>A",
          "hgvs_p": "p.Gly1013Ser",
          "transcript": "XM_017028317.2",
          "protein_id": "XP_016883806.1",
          "transcript_support_level": null,
          "aa_start": 1013,
          "aa_end": null,
          "aa_length": 1055,
          "cds_start": 3037,
          "cds_end": null,
          "cds_length": 3168,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017028317.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LTN1",
          "gene_hgnc_id": 13082,
          "hgvs_c": "c.2653G>A",
          "hgvs_p": "p.Gly885Ser",
          "transcript": "XM_047440742.1",
          "protein_id": "XP_047296698.1",
          "transcript_support_level": null,
          "aa_start": 885,
          "aa_end": null,
          "aa_length": 927,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 2784,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440742.1"
        }
      ],
      "gene_symbol": "LTN1",
      "gene_hgnc_id": 13082,
      "dbsnp": "rs777230492",
      "frequency_reference_population": 0.000032852142,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 48,
      "gnomad_exomes_af": 0.0000328521,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 48,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.298855721950531,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.12999999523162842,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.286,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.7068,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.633,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.13,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_015565.3",
          "gene_symbol": "LTN1",
          "hgnc_id": 13082,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.5170G>A",
          "hgvs_p": "p.Gly1724Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}