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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-34792277-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=34792277&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 34792277,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000675419.1",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1301A>C",
          "hgvs_p": "p.Asn434Thr",
          "transcript": "NM_001754.5",
          "protein_id": "NP_001745.2",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1301,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1495,
          "cdna_end": null,
          "cdna_length": 5971,
          "mane_select": "ENST00000675419.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1301A>C",
          "hgvs_p": "p.Asn434Thr",
          "transcript": "ENST00000675419.1",
          "protein_id": "ENSP00000501943.1",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1301,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1495,
          "cdna_end": null,
          "cdna_length": 5971,
          "mane_select": "NM_001754.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1301A>C",
          "hgvs_p": "p.Asn434Thr",
          "transcript": "ENST00000300305.7",
          "protein_id": "ENSP00000300305.3",
          "transcript_support_level": 1,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1301,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1746,
          "cdna_end": null,
          "cdna_length": 6222,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1220A>C",
          "hgvs_p": "p.Asn407Thr",
          "transcript": "ENST00000344691.8",
          "protein_id": "ENSP00000340690.4",
          "transcript_support_level": 1,
          "aa_start": 407,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 1220,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": 2798,
          "cdna_end": null,
          "cdna_length": 7274,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "n.*891A>C",
          "hgvs_p": null,
          "transcript": "ENST00000482318.5",
          "protein_id": "ENSP00000477067.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1710,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "n.*891A>C",
          "hgvs_p": null,
          "transcript": "ENST00000482318.5",
          "protein_id": "ENSP00000477067.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1710,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1220A>C",
          "hgvs_p": "p.Asn407Thr",
          "transcript": "NM_001001890.3",
          "protein_id": "NP_001001890.1",
          "transcript_support_level": null,
          "aa_start": 407,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 1220,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": 2807,
          "cdna_end": null,
          "cdna_length": 7283,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1028A>C",
          "hgvs_p": "p.Asn343Thr",
          "transcript": "ENST00000399240.5",
          "protein_id": "ENSP00000382184.1",
          "transcript_support_level": 3,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 389,
          "cds_start": 1028,
          "cds_end": null,
          "cds_length": 1170,
          "cdna_start": 1099,
          "cdna_end": null,
          "cdna_length": 1590,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1301A>C",
          "hgvs_p": "p.Asn434Thr",
          "transcript": "XM_011529766.3",
          "protein_id": "XP_011528068.1",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1301,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1582,
          "cdna_end": null,
          "cdna_length": 6058,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1301A>C",
          "hgvs_p": "p.Asn434Thr",
          "transcript": "XM_047441007.1",
          "protein_id": "XP_047296963.1",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1301,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1704,
          "cdna_end": null,
          "cdna_length": 6180,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1265A>C",
          "hgvs_p": "p.Asn422Thr",
          "transcript": "XM_005261068.4",
          "protein_id": "XP_005261125.1",
          "transcript_support_level": null,
          "aa_start": 422,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 1265,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": 1288,
          "cdna_end": null,
          "cdna_length": 5764,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1262A>C",
          "hgvs_p": "p.Asn421Thr",
          "transcript": "XM_011529767.3",
          "protein_id": "XP_011528069.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": 1456,
          "cdna_end": null,
          "cdna_length": 5932,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1262A>C",
          "hgvs_p": "p.Asn421Thr",
          "transcript": "XM_047441009.1",
          "protein_id": "XP_047296965.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": 1665,
          "cdna_end": null,
          "cdna_length": 6141,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1262A>C",
          "hgvs_p": "p.Asn421Thr",
          "transcript": "XM_047441010.1",
          "protein_id": "XP_047296966.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": 1543,
          "cdna_end": null,
          "cdna_length": 6019,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1109A>C",
          "hgvs_p": "p.Asn370Thr",
          "transcript": "XM_005261069.5",
          "protein_id": "XP_005261126.1",
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          "aa_length": 416,
          "cds_start": 1109,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": 1303,
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          "cdna_length": 5779,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1109A>C",
          "hgvs_p": "p.Asn370Thr",
          "transcript": "XM_047441011.1",
          "protein_id": "XP_047296967.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1109,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": 1512,
          "cdna_end": null,
          "cdna_length": 5988,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1109A>C",
          "hgvs_p": "p.Asn370Thr",
          "transcript": "XM_047441012.1",
          "protein_id": "XP_047296968.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1109,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": 1390,
          "cdna_end": null,
          "cdna_length": 5866,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1073A>C",
          "hgvs_p": "p.Asn358Thr",
          "transcript": "XM_047441013.1",
          "protein_id": "XP_047296969.1",
          "transcript_support_level": null,
          "aa_start": 358,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 1073,
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          "cdna_start": 1096,
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          "cdna_length": 5572,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1070A>C",
          "hgvs_p": "p.Asn357Thr",
          "transcript": "XM_011529768.3",
          "protein_id": "XP_011528070.1",
          "transcript_support_level": null,
          "aa_start": 357,
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          "aa_length": 403,
          "cds_start": 1070,
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          "cds_length": 1212,
          "cdna_start": 1264,
          "cdna_end": null,
          "cdna_length": 5740,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1070A>C",
          "hgvs_p": "p.Asn357Thr",
          "transcript": "XM_047441014.1",
          "protein_id": "XP_047296970.1",
          "transcript_support_level": null,
          "aa_start": 357,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1070,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": 1473,
          "cdna_end": null,
          "cdna_length": 5949,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1",
          "gene_hgnc_id": 10471,
          "hgvs_c": "c.1070A>C",
          "hgvs_p": "p.Asn357Thr",
          "transcript": "XM_047441015.1",
          "protein_id": "XP_047296971.1",
          "transcript_support_level": null,
          "aa_start": 357,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1070,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": 1351,
          "cdna_end": null,
          "cdna_length": 5827,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RUNX1",
      "gene_hgnc_id": 10471,
      "dbsnp": "rs748885850",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.20416492223739624,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.127,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1154,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.616,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000675419.1",
          "gene_symbol": "RUNX1",
          "hgnc_id": 10471,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1301A>C",
          "hgvs_p": "p.Asn434Thr"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}