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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-36036228-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=36036228&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 36036228,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_017438.5",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "NM_017438.5",
          "protein_id": "NP_059134.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000332131.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017438.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000332131.9",
          "protein_id": "ENSP00000329189.4",
          "transcript_support_level": 2,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017438.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000332131.9"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1140A>T",
          "hgvs_p": "p.Lys380Asn",
          "transcript": "ENST00000399212.5",
          "protein_id": "ENSP00000382161.1",
          "transcript_support_level": 1,
          "aa_start": 380,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1140,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000399212.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "n.1198A>T",
          "hgvs_p": null,
          "transcript": "ENST00000481477.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000481477.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1296A>T",
          "hgvs_p": "p.Lys432Asn",
          "transcript": "ENST00000962399.1",
          "protein_id": "ENSP00000632458.1",
          "transcript_support_level": null,
          "aa_start": 432,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 1296,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962399.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000399215.5",
          "protein_id": "ENSP00000382163.1",
          "transcript_support_level": 2,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000399215.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000902513.1",
          "protein_id": "ENSP00000572572.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902513.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000902515.1",
          "protein_id": "ENSP00000572574.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902515.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000902516.1",
          "protein_id": "ENSP00000572575.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902516.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000902517.1",
          "protein_id": "ENSP00000572576.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902517.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000931866.1",
          "protein_id": "ENSP00000601925.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931866.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000931867.1",
          "protein_id": "ENSP00000601926.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931867.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000931868.1",
          "protein_id": "ENSP00000601927.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931868.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000931869.1",
          "protein_id": "ENSP00000601928.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931869.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1212A>T",
          "hgvs_p": "p.Lys404Asn",
          "transcript": "ENST00000962398.1",
          "protein_id": "ENSP00000632457.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962398.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1182A>T",
          "hgvs_p": "p.Lys394Asn",
          "transcript": "ENST00000931865.1",
          "protein_id": "ENSP00000601924.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 430,
          "cds_start": 1182,
          "cds_end": null,
          "cds_length": 1293,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931865.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1182A>T",
          "hgvs_p": "p.Lys394Asn",
          "transcript": "ENST00000962396.1",
          "protein_id": "ENSP00000632455.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 430,
          "cds_start": 1182,
          "cds_end": null,
          "cds_length": 1293,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962396.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1140A>T",
          "hgvs_p": "p.Lys380Asn",
          "transcript": "NM_001286752.2",
          "protein_id": "NP_001273681.1",
          "transcript_support_level": null,
          "aa_start": 380,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1140,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286752.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1116A>T",
          "hgvs_p": "p.Lys372Asn",
          "transcript": "ENST00000902514.1",
          "protein_id": "ENSP00000572573.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 1116,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902514.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD4",
          "gene_hgnc_id": 1258,
          "hgvs_c": "c.1116A>T",
          "hgvs_p": "p.Lys372Asn",
          "transcript": "ENST00000931864.1",
          "protein_id": "ENSP00000601923.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 1116,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": null,
          "cdna_end": null,
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      "gnomad_exomes_af": 0.00393552,
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      "gnomad_genomes_homalt": 0,
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      "computational_score_selected": 0.003034442663192749,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.015,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1111,
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      "bayesdelnoaf_score": -0.72,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -2.158,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": -10,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Moderate,BS2",
      "acmg_by_gene": [
        {
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          "benign_score": 10,
          "pathogenic_score": 0,
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            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_017438.5",
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          "hgnc_id": 1258,
          "effects": [
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          ],
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          "hgvs_p": "p.Lys404Asn"
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      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}