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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-43793384-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=43793384&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 43793384,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_003683.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "NM_003683.6",
          "protein_id": "NP_003674.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000497547.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003683.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000497547.2",
          "protein_id": "ENSP00000417464.1",
          "transcript_support_level": 1,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003683.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000497547.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "n.454C>T",
          "hgvs_p": null,
          "transcript": "ENST00000467112.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000467112.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000856885.1",
          "protein_id": "ENSP00000526944.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856885.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000856887.1",
          "protein_id": "ENSP00000526946.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 459,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1380,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856887.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000917587.1",
          "protein_id": "ENSP00000587646.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917587.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000856884.1",
          "protein_id": "ENSP00000526943.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856884.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000917588.1",
          "protein_id": "ENSP00000587647.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 439,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1320,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917588.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "ENST00000917589.1",
          "protein_id": "ENSP00000587648.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 423,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1272,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917589.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys",
          "transcript": "XM_017028485.3",
          "protein_id": "XP_016883974.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 415,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 1248,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017028485.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.217-1805C>T",
          "hgvs_p": null,
          "transcript": "ENST00000917586.1",
          "protein_id": "ENSP00000587645.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917586.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "c.162+2006C>T",
          "hgvs_p": null,
          "transcript": "ENST00000856886.1",
          "protein_id": "ENSP00000526945.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856886.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "n.390C>T",
          "hgvs_p": null,
          "transcript": "ENST00000475534.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000475534.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "n.*243C>T",
          "hgvs_p": null,
          "transcript": "ENST00000483896.5",
          "protein_id": "ENSP00000426898.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000483896.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "n.982C>T",
          "hgvs_p": null,
          "transcript": "ENST00000492638.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000492638.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RRP1",
          "gene_hgnc_id": 18785,
          "hgvs_c": "n.*243C>T",
          "hgvs_p": null,
          "transcript": "ENST00000483896.5",
          "protein_id": "ENSP00000426898.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000483896.5"
        }
      ],
      "gene_symbol": "RRP1",
      "gene_hgnc_id": 18785,
      "dbsnp": "rs763767114",
      "frequency_reference_population": 0.000018592844,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 30,
      "gnomad_exomes_af": 0.0000198453,
      "gnomad_genomes_af": 0.00000656953,
      "gnomad_exomes_ac": 29,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9821521043777466,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.903,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8087,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.28,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 1.942,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_003683.6",
          "gene_symbol": "RRP1",
          "hgnc_id": 18785,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.340C>T",
          "hgvs_p": "p.Arg114Cys"
        }
      ],
      "clinvar_disease": "Breast ductal adenocarcinoma",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Breast ductal adenocarcinoma",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}