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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-44889397-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=44889397&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 44889397,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000652462.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "NM_000211.5",
          "protein_id": "NP_000202.3",
          "transcript_support_level": null,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 1838,
          "cdna_end": null,
          "cdna_length": 2807,
          "mane_select": "ENST00000652462.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000652462.1",
          "protein_id": "ENSP00000498780.1",
          "transcript_support_level": null,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 1838,
          "cdna_end": null,
          "cdna_length": 2807,
          "mane_select": "NM_000211.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1828C>T",
          "hgvs_p": "p.Arg610Trp",
          "transcript": "ENST00000302347.10",
          "protein_id": "ENSP00000303242.6",
          "transcript_support_level": 1,
          "aa_start": 610,
          "aa_end": null,
          "aa_length": 793,
          "cds_start": 1828,
          "cds_end": null,
          "cds_length": 2382,
          "cdna_start": 1910,
          "cdna_end": null,
          "cdna_length": 2867,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000397852.5",
          "protein_id": "ENSP00000380950.1",
          "transcript_support_level": 1,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 1800,
          "cdna_end": null,
          "cdna_length": 2769,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1585C>T",
          "hgvs_p": "p.Arg529Trp",
          "transcript": "ENST00000397854.7",
          "protein_id": "ENSP00000380952.3",
          "transcript_support_level": 1,
          "aa_start": 529,
          "aa_end": null,
          "aa_length": 712,
          "cds_start": 1585,
          "cds_end": null,
          "cds_length": 2139,
          "cdna_start": 1623,
          "cdna_end": null,
          "cdna_length": 2592,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "n.3812C>T",
          "hgvs_p": null,
          "transcript": "ENST00000498666.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4781,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "NM_001127491.3",
          "protein_id": "NP_001120963.2",
          "transcript_support_level": null,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 1921,
          "cdna_end": null,
          "cdna_length": 2890,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000355153.8",
          "protein_id": "ENSP00000347279.4",
          "transcript_support_level": 2,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 1944,
          "cdna_end": null,
          "cdna_length": 2913,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000397850.6",
          "protein_id": "ENSP00000380948.2",
          "transcript_support_level": 5,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 2209,
          "cdna_end": null,
          "cdna_length": 3178,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000397857.5",
          "protein_id": "ENSP00000380955.1",
          "transcript_support_level": 5,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 1844,
          "cdna_end": null,
          "cdna_length": 2813,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Arg517Trp",
          "transcript": "NM_001303238.2",
          "protein_id": "NP_001290167.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 700,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2103,
          "cdna_start": 1881,
          "cdna_end": null,
          "cdna_length": 2850,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Arg517Trp",
          "transcript": "XM_006724001.3",
          "protein_id": "XP_006724064.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 700,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2103,
          "cdna_start": 1883,
          "cdna_end": null,
          "cdna_length": 2852,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Arg517Trp",
          "transcript": "XM_047440763.1",
          "protein_id": "XP_047296719.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 700,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2103,
          "cdna_start": 1926,
          "cdna_end": null,
          "cdna_length": 2895,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "n.1156C>T",
          "hgvs_p": null,
          "transcript": "ENST00000475170.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2125,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "n.*1583C>T",
          "hgvs_p": null,
          "transcript": "ENST00000523323.5",
          "protein_id": "ENSP00000427732.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2823,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "n.963C>T",
          "hgvs_p": null,
          "transcript": "ENST00000696946.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1920,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB2",
          "gene_hgnc_id": 6155,
          "hgvs_c": "n.*1583C>T",
          "hgvs_p": null,
          "transcript": "ENST00000523323.5",
          "protein_id": "ENSP00000427732.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2823,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ITGB2",
      "gene_hgnc_id": 6155,
      "dbsnp": "rs5030672",
      "frequency_reference_population": 0.008453586,
      "hom_count_reference_population": 128,
      "allele_count_reference_population": 13632,
      "gnomad_exomes_af": 0.00851046,
      "gnomad_genomes_af": 0.00790805,
      "gnomad_exomes_ac": 12428,
      "gnomad_genomes_ac": 1204,
      "gnomad_exomes_homalt": 105,
      "gnomad_genomes_homalt": 23,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.05598476529121399,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.489,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2139,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": -0.735,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -18,
      "acmg_classification": "Benign",
      "acmg_criteria": "PM1,BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -18,
          "benign_score": 20,
          "pathogenic_score": 2,
          "criteria": [
            "PM1",
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000652462.1",
          "gene_symbol": "ITGB2",
          "hgnc_id": 6155,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp"
        }
      ],
      "clinvar_disease": "ITGB2-related disorder,Leukocyte adhesion deficiency 1,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:4 B:2",
      "phenotype_combined": "Leukocyte adhesion deficiency 1|not provided|not specified|ITGB2-related disorder",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}