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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-45487495-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=45487495&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 45487495,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_130444.3",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "NM_001379500.1",
          "protein_id": "NP_001366429.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1339,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000651438.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001379500.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000651438.1",
          "protein_id": "ENSP00000498485.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1339,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001379500.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000651438.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.2422G>A",
          "hgvs_p": "p.Ala808Thr",
          "transcript": "ENST00000355480.10",
          "protein_id": "ENSP00000347665.5",
          "transcript_support_level": 1,
          "aa_start": 808,
          "aa_end": null,
          "aa_length": 1519,
          "cds_start": 2422,
          "cds_end": null,
          "cds_length": 4560,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355480.10"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.3127G>A",
          "hgvs_p": "p.Ala1043Thr",
          "transcript": "NM_130444.3",
          "protein_id": "NP_569711.2",
          "transcript_support_level": null,
          "aa_start": 1043,
          "aa_end": null,
          "aa_length": 1754,
          "cds_start": 3127,
          "cds_end": null,
          "cds_length": 5265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130444.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.3127G>A",
          "hgvs_p": "p.Ala1043Thr",
          "transcript": "ENST00000359759.8",
          "protein_id": "ENSP00000352798.4",
          "transcript_support_level": 5,
          "aa_start": 1043,
          "aa_end": null,
          "aa_length": 1754,
          "cds_start": 3127,
          "cds_end": null,
          "cds_length": 5265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000359759.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.2422G>A",
          "hgvs_p": "p.Ala808Thr",
          "transcript": "NM_030582.4",
          "protein_id": "NP_085059.2",
          "transcript_support_level": null,
          "aa_start": 808,
          "aa_end": null,
          "aa_length": 1519,
          "cds_start": 2422,
          "cds_end": null,
          "cds_length": 4560,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_030582.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.2314G>A",
          "hgvs_p": "p.Ala772Thr",
          "transcript": "ENST00000859062.1",
          "protein_id": "ENSP00000529121.1",
          "transcript_support_level": null,
          "aa_start": 772,
          "aa_end": null,
          "aa_length": 1483,
          "cds_start": 2314,
          "cds_end": null,
          "cds_length": 4452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859062.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859069.1",
          "protein_id": "ENSP00000529128.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1362,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859069.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859068.1",
          "protein_id": "ENSP00000529127.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1359,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4080,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859068.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859063.1",
          "protein_id": "ENSP00000529122.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1357,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4074,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859063.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859060.1",
          "protein_id": "ENSP00000529119.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1350,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4053,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859060.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000970099.1",
          "protein_id": "ENSP00000640158.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1336,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 4011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970099.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859066.1",
          "protein_id": "ENSP00000529125.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1331,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 3996,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859066.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000930597.1",
          "protein_id": "ENSP00000600656.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1330,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 3993,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930597.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000970097.1",
          "protein_id": "ENSP00000640156.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1330,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 3993,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970097.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859067.1",
          "protein_id": "ENSP00000529126.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1329,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 3990,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859067.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000859059.1",
          "protein_id": "ENSP00000529118.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1327,
          "cds_start": 1882,
          "cds_end": null,
          "cds_length": 3984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000859059.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1828G>A",
          "hgvs_p": "p.Ala610Thr",
          "transcript": "ENST00000930601.1",
          "protein_id": "ENSP00000600660.1",
          "transcript_support_level": null,
          "aa_start": 610,
          "aa_end": null,
          "aa_length": 1321,
          "cds_start": 1828,
          "cds_end": null,
          "cds_length": 3966,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930601.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1795G>A",
          "hgvs_p": "p.Ala599Thr",
          "transcript": "ENST00000859064.1",
          "protein_id": "ENSP00000529123.1",
          "transcript_support_level": null,
          "aa_start": 599,
          "aa_end": null,
          "aa_length": 1310,
          "cds_start": 1795,
          "cds_end": null,
          "cds_length": 3933,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859064.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL18A1",
          "gene_hgnc_id": 2195,
          "hgvs_c": "c.1882G>A",
          "hgvs_p": "p.Ala628Thr",
          "transcript": "ENST00000930596.1",
          "protein_id": "ENSP00000600655.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 1293,
          "cds_start": 1882,
          "cds_end": null,
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          "gene_symbol": "COL18A1",
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          "protein_id": "ENSP00000600653.1",
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          "feature": "ENST00000930594.1"
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      ],
      "gene_symbol": "COL18A1",
      "gene_hgnc_id": 2195,
      "dbsnp": "rs181012655",
      "frequency_reference_population": 0.0001258512,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 203,
      "gnomad_exomes_af": 0.0000725687,
      "gnomad_genomes_af": 0.000636767,
      "gnomad_exomes_ac": 106,
      "gnomad_genomes_ac": 97,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.006442457437515259,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.137,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0724,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.37,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.794,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_130444.3",
          "gene_symbol": "COL18A1",
          "hgnc_id": 2195,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.3127G>A",
          "hgvs_p": "p.Ala1043Thr"
        }
      ],
      "clinvar_disease": "not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:1",
      "phenotype_combined": "not specified|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}