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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 21-46132216-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=21&pos=46132216&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "21",
      "pos": 46132216,
      "ref": "A",
      "alt": "G",
      "effect": "synonymous_variant",
      "transcript": "NM_001849.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "NM_001849.4",
          "protein_id": "NP_001840.3",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000300527.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001849.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000300527.9",
          "protein_id": "ENSP00000300527.4",
          "transcript_support_level": 1,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001849.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000300527.9"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2919A>G",
          "hgvs_p": "p.Thr973Thr",
          "transcript": "ENST00000857098.1",
          "protein_id": "ENSP00000527157.1",
          "transcript_support_level": null,
          "aa_start": 973,
          "aa_end": null,
          "aa_length": 1084,
          "cds_start": 2919,
          "cds_end": null,
          "cds_length": 3255,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857098.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2886A>G",
          "hgvs_p": "p.Thr962Thr",
          "transcript": "ENST00000857103.1",
          "protein_id": "ENSP00000527162.1",
          "transcript_support_level": null,
          "aa_start": 962,
          "aa_end": null,
          "aa_length": 1073,
          "cds_start": 2886,
          "cds_end": null,
          "cds_length": 3222,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857103.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2841A>G",
          "hgvs_p": "p.Thr947Thr",
          "transcript": "ENST00000857092.1",
          "protein_id": "ENSP00000527151.1",
          "transcript_support_level": null,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 1058,
          "cds_start": 2841,
          "cds_end": null,
          "cds_length": 3177,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857092.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2793A>G",
          "hgvs_p": "p.Thr931Thr",
          "transcript": "ENST00000857106.1",
          "protein_id": "ENSP00000527165.1",
          "transcript_support_level": null,
          "aa_start": 931,
          "aa_end": null,
          "aa_length": 1042,
          "cds_start": 2793,
          "cds_end": null,
          "cds_length": 3129,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857106.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2766A>G",
          "hgvs_p": "p.Thr922Thr",
          "transcript": "ENST00000968880.1",
          "protein_id": "ENSP00000638939.1",
          "transcript_support_level": null,
          "aa_start": 922,
          "aa_end": null,
          "aa_length": 1033,
          "cds_start": 2766,
          "cds_end": null,
          "cds_length": 3102,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968880.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2754A>G",
          "hgvs_p": "p.Thr918Thr",
          "transcript": "ENST00000857101.1",
          "protein_id": "ENSP00000527160.1",
          "transcript_support_level": null,
          "aa_start": 918,
          "aa_end": null,
          "aa_length": 1029,
          "cds_start": 2754,
          "cds_end": null,
          "cds_length": 3090,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857101.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857090.1",
          "protein_id": "ENSP00000527149.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857090.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857091.1",
          "protein_id": "ENSP00000527150.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857091.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857094.1",
          "protein_id": "ENSP00000527153.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857094.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857096.1",
          "protein_id": "ENSP00000527155.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857096.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857100.1",
          "protein_id": "ENSP00000527159.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857100.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857108.1",
          "protein_id": "ENSP00000527167.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857108.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000857109.1",
          "protein_id": "ENSP00000527168.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857109.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000968878.1",
          "protein_id": "ENSP00000638937.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968878.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000968883.1",
          "protein_id": "ENSP00000638942.1",
          "transcript_support_level": null,
          "aa_start": 908,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000968883.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr",
          "transcript": "ENST00000968884.1",
          "protein_id": "ENSP00000638943.1",
          "transcript_support_level": null,
          "aa_start": 908,
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          "aa_length": 1019,
          "cds_start": 2724,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000968884.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2718A>G",
          "hgvs_p": "p.Thr906Thr",
          "transcript": "ENST00000968879.1",
          "protein_id": "ENSP00000638938.1",
          "transcript_support_level": null,
          "aa_start": 906,
          "aa_end": null,
          "aa_length": 1017,
          "cds_start": 2718,
          "cds_end": null,
          "cds_length": 3054,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968879.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COL6A2",
          "gene_hgnc_id": 2212,
          "hgvs_c": "c.2715A>G",
          "hgvs_p": "p.Thr905Thr",
          "transcript": "ENST00000934972.1",
          "protein_id": "ENSP00000605031.1",
          "transcript_support_level": null,
          "aa_start": 905,
          "aa_end": null,
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      ],
      "gene_symbol": "COL6A2",
      "gene_hgnc_id": 2212,
      "dbsnp": "rs9977394",
      "frequency_reference_population": 0.10704717,
      "hom_count_reference_population": 10849,
      "allele_count_reference_population": 170451,
      "gnomad_exomes_af": 0.103885,
      "gnomad_genomes_af": 0.136969,
      "gnomad_exomes_ac": 149607,
      "gnomad_genomes_ac": 20844,
      "gnomad_exomes_homalt": 8978,
      "gnomad_genomes_homalt": 1871,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.9300000071525574,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.93,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.037,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -21,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BP7,BA1",
      "acmg_by_gene": [
        {
          "score": -21,
          "benign_score": 21,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BP7",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001849.4",
          "gene_symbol": "COL6A2",
          "hgnc_id": 2212,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.2724A>G",
          "hgvs_p": "p.Thr908Thr"
        }
      ],
      "clinvar_disease": "Bethlem myopathy 1A,Collagen 6-related myopathy,Glutamate formiminotransferase deficiency,Myosclerosis,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:10",
      "phenotype_combined": "not specified|Myosclerosis|Glutamate formiminotransferase deficiency|Collagen 6-related myopathy|not provided|Bethlem myopathy 1A",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}