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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-21682490-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=21682490&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 21682490,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_148176.3",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "NM_014337.4",
          "protein_id": "NP_055152.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000398831.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014337.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000398831.8",
          "protein_id": "ENSP00000381812.3",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014337.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000398831.8"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000626352.2",
          "protein_id": "ENSP00000486725.1",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000626352.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000335025.12",
          "protein_id": "ENSP00000334553.7",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000335025.12"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000406385.1",
          "protein_id": "ENSP00000384299.1",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000406385.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "n.1135C>A",
          "hgvs_p": null,
          "transcript": "ENST00000496819.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000496819.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000680094.1",
          "protein_id": "ENSP00000506368.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 614,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1845,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680094.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000681338.1",
          "protein_id": "ENSP00000505107.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 573,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1722,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000681338.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000679477.1",
          "protein_id": "ENSP00000505978.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 549,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1650,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679477.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "NM_148176.3",
          "protein_id": "NP_680481.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_148176.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "NM_001317996.2",
          "protein_id": "NP_001304925.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317996.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "NM_148175.3",
          "protein_id": "NP_680480.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_148175.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000679534.1",
          "protein_id": "ENSP00000504893.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679534.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000679540.1",
          "protein_id": "ENSP00000506328.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679540.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000679795.1",
          "protein_id": "ENSP00000506144.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679795.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000680061.1",
          "protein_id": "ENSP00000505910.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680061.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000680393.1",
          "protein_id": "ENSP00000506542.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680393.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000681956.1",
          "protein_id": "ENSP00000506158.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000681956.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu",
          "transcript": "ENST00000679827.1",
          "protein_id": "ENSP00000504929.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 441,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679827.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL2",
          "gene_hgnc_id": 9261,
          "hgvs_c": "c.378C>A",
          "hgvs_p": "p.Asp126Glu",
          "transcript": "ENST00000680109.1",
          "protein_id": "ENSP00000506219.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 499,
          "cds_start": 378,
          "cds_end": null,
          "cds_length": 1500,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680109.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
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      "gene_symbol": "PPIL2",
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      "dbsnp": "rs755435729",
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      "gnomad_exomes_af": 0.00000137224,
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      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.3591751158237457,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.278,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.318,
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      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.251,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
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            "BP4"
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          "verdict": "Uncertain_significance",
          "transcript": "NM_148176.3",
          "gene_symbol": "PPIL2",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.441C>A",
          "hgvs_p": "p.Asp147Glu"
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      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}