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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-33274611-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=33274611&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 33274611,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000397394.8",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_133642.5",
          "protein_id": "NP_598397.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2651,
          "cdna_end": null,
          "cdna_length": 4753,
          "mane_select": "ENST00000397394.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "ENST00000397394.8",
          "protein_id": "ENSP00000380549.2",
          "transcript_support_level": 5,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2651,
          "cdna_end": null,
          "cdna_length": 4753,
          "mane_select": "NM_133642.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "ENST00000354992.7",
          "protein_id": "ENSP00000347088.2",
          "transcript_support_level": 1,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2659,
          "cdna_end": null,
          "cdna_length": 4193,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.1931T>C",
          "hgvs_p": "p.Ile644Thr",
          "transcript": "ENST00000402320.6",
          "protein_id": "ENSP00000385223.1",
          "transcript_support_level": 1,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 704,
          "cds_start": 1931,
          "cds_end": null,
          "cds_length": 2115,
          "cdna_start": 2433,
          "cdna_end": null,
          "cdna_length": 3967,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_001362949.2",
          "protein_id": "NP_001349878.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2436,
          "cdna_end": null,
          "cdna_length": 4538,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_001362951.2",
          "protein_id": "NP_001349880.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2256,
          "cdna_end": null,
          "cdna_length": 4358,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_001362953.2",
          "protein_id": "NP_001349882.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2373,
          "cdna_end": null,
          "cdna_length": 4475,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_001378624.1",
          "protein_id": "NP_001365553.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2319,
          "cdna_end": null,
          "cdna_length": 4421,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_001378625.1",
          "protein_id": "NP_001365554.1",
          "transcript_support_level": null,
          "aa_start": 696,
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          "aa_length": 756,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": 2454,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
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        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "LARGE1",
          "gene_hgnc_id": 6511,
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_001378626.1",
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          "aa_start": 696,
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          "cds_start": 2087,
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          "cdna_start": 2391,
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        {
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          "gene_symbol": "LARGE1",
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          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr",
          "transcript": "NM_004737.7",
          "protein_id": "NP_004728.1",
          "transcript_support_level": null,
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          "cds_start": 2087,
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        {
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          "hgvs_c": "c.2087T>C",
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        {
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          "gene_symbol": "LARGE1",
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          "hgvs_c": "c.2087T>C",
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          "transcript": "ENST00000676070.1",
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        {
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        {
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          "gene_symbol": "LARGE1",
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          "transcript": "ENST00000676370.1",
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          "gene_symbol": "LARGE1",
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          "hgvs_c": "c.1961T>C",
          "hgvs_p": "p.Ile654Thr",
          "transcript": "ENST00000674668.1",
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          "gene_symbol": "LARGE1",
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          "transcript": "ENST00000674789.1",
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        {
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          ],
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      ],
      "gene_symbol": "LARGE1",
      "gene_hgnc_id": 6511,
      "dbsnp": "rs200212868",
      "frequency_reference_population": 0.000004336836,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 7,
      "gnomad_exomes_af": 0.00000342029,
      "gnomad_genomes_af": 0.0000131394,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.08074402809143066,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.085,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0989,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.6,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.06,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
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            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000397394.8",
          "gene_symbol": "LARGE1",
          "hgnc_id": 6511,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2087T>C",
          "hgvs_p": "p.Ile696Thr"
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      ],
      "clinvar_disease": "Muscular dystrophy-dystroglycanopathy type B6",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Muscular dystrophy-dystroglycanopathy type B6",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}