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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-39314810-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=39314810&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 39314810,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_000967.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "NM_000967.4",
          "protein_id": "NP_000958.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000216146.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000967.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000216146.9",
          "protein_id": "ENSP00000346001.3",
          "transcript_support_level": 1,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000967.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000216146.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190His",
          "transcript": "ENST00000401609.5",
          "protein_id": "ENSP00000386101.1",
          "transcript_support_level": 1,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000401609.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "n.1565G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465618.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000465618.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "n.156G>A",
          "hgvs_p": null,
          "transcript": "ENST00000467105.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000467105.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000926233.1",
          "protein_id": "ENSP00000596292.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 429,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1290,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926233.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.746G>A",
          "hgvs_p": "p.Arg249His",
          "transcript": "ENST00000926234.1",
          "protein_id": "ENSP00000596293.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926234.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000871470.1",
          "protein_id": "ENSP00000541529.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 406,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1221,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871470.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000971220.1",
          "protein_id": "ENSP00000641279.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971220.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000871474.1",
          "protein_id": "ENSP00000541533.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871474.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000871477.1",
          "protein_id": "ENSP00000541536.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871477.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000871476.1",
          "protein_id": "ENSP00000541535.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871476.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000926227.1",
          "protein_id": "ENSP00000596286.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926227.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234His",
          "transcript": "ENST00000871471.1",
          "protein_id": "ENSP00000541530.1",
          "transcript_support_level": null,
          "aa_start": 234,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 701,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871471.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.698G>A",
          "hgvs_p": "p.Arg233His",
          "transcript": "ENST00000871469.1",
          "protein_id": "ENSP00000541528.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 394,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 1185,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871469.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.683G>A",
          "hgvs_p": "p.Arg228His",
          "transcript": "ENST00000926230.1",
          "protein_id": "ENSP00000596289.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 389,
          "cds_start": 683,
          "cds_end": null,
          "cds_length": 1170,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926230.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.680G>A",
          "hgvs_p": "p.Arg227His",
          "transcript": "ENST00000871473.1",
          "protein_id": "ENSP00000541532.1",
          "transcript_support_level": null,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": 680,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871473.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000871468.1",
          "protein_id": "ENSP00000541527.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871468.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.725G>A",
          "hgvs_p": "p.Arg242His",
          "transcript": "ENST00000871472.1",
          "protein_id": "ENSP00000541531.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 369,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1110,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871472.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3",
          "gene_hgnc_id": 10332,
          "hgvs_c": "c.578G>A",
          "hgvs_p": "p.Arg193His",
          "transcript": "NM_001033853.2",
          "protein_id": "NP_001029025.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 354,
          "cds_start": 578,
          "cds_end": null,
          "cds_length": 1065,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001033853.2"
        },
        {
          "aa_ref": "R",
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      ],
      "gene_symbol": "RPL3",
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      "dbsnp": "rs1480072027",
      "frequency_reference_population": 0.000023549652,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 38,
      "gnomad_exomes_af": 0.0000246316,
      "gnomad_genomes_af": 0.0000131513,
      "gnomad_exomes_ac": 36,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7143316268920898,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.357,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9941,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.03,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.759,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
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          ],
          "verdict": "Likely_benign",
          "transcript": "NM_000967.4",
          "gene_symbol": "RPL3",
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          "effects": [
            "missense_variant"
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          "inheritance_mode": "AD",
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          "hgvs_p": "p.Arg242His"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}