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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-39521492-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=39521492&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 39521492,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001675.4",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "NM_182810.3",
          "protein_id": "NP_877962.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 329,
          "cdna_end": null,
          "cdna_length": 1419,
          "mane_select": "ENST00000674920.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_182810.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000674920.3",
          "protein_id": "ENSP00000501863.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 329,
          "cdna_end": null,
          "cdna_length": 1419,
          "mane_select": "NM_182810.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674920.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000337304.2",
          "protein_id": "ENSP00000336790.2",
          "transcript_support_level": 1,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 929,
          "cdna_end": null,
          "cdna_length": 2019,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000337304.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000396680.3",
          "protein_id": "ENSP00000379912.1",
          "transcript_support_level": 1,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 411,
          "cdna_end": null,
          "cdna_length": 1486,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396680.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "NM_001675.4",
          "protein_id": "NP_001666.2",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 934,
          "cdna_end": null,
          "cdna_length": 2027,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001675.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000404241.7",
          "protein_id": "ENSP00000384587.2",
          "transcript_support_level": 5,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 820,
          "cdna_end": null,
          "cdna_length": 1913,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000404241.7"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000674568.2",
          "protein_id": "ENSP00000501783.2",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 234,
          "cdna_end": null,
          "cdna_length": 1243,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674568.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000674835.2",
          "protein_id": "ENSP00000502610.2",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 266,
          "cdna_end": null,
          "cdna_length": 1275,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674835.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000676346.2",
          "protein_id": "ENSP00000502400.2",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 297,
          "cdna_end": null,
          "cdna_length": 1306,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676346.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000679776.1",
          "protein_id": "ENSP00000505360.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 229,
          "cdna_end": null,
          "cdna_length": 1238,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679776.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000921524.1",
          "protein_id": "ENSP00000591583.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": 535,
          "cdna_end": null,
          "cdna_length": 1632,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000921524.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000921525.1",
          "protein_id": "ENSP00000591584.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
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          "cds_length": 1056,
          "cdna_start": 238,
          "cdna_end": null,
          "cdna_length": 1325,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000921525.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000921526.1",
          "protein_id": "ENSP00000591585.1",
          "transcript_support_level": null,
          "aa_start": 16,
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          "cds_start": 47,
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          "cds_length": 1056,
          "cdna_start": 561,
          "cdna_end": null,
          "cdna_length": 1654,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000921526.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000921527.1",
          "protein_id": "ENSP00000591586.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
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          "cds_start": 47,
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          "cdna_start": 591,
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          "cdna_length": 1684,
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          "biotype": "protein_coding",
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000921528.1",
          "protein_id": "ENSP00000591587.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
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          "cdna_start": 264,
          "cdna_end": null,
          "cdna_length": 1354,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000921528.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000921529.1",
          "protein_id": "ENSP00000591588.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 47,
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          "cdna_start": 334,
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          "cdna_length": 1431,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000960424.1",
          "protein_id": "ENSP00000630483.1",
          "transcript_support_level": null,
          "aa_start": 16,
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          "cdna_start": 545,
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          "biotype": "protein_coding",
          "feature": "ENST00000960424.1"
        },
        {
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
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          "transcript": "ENST00000960425.1",
          "protein_id": "ENSP00000630484.1",
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          "cdna_start": 329,
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          "cdna_length": 1416,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "L",
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          ],
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          "intron_rank": null,
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          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000960426.1",
          "protein_id": "ENSP00000630485.1",
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          "cdna_start": 331,
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          "cdna_length": 1423,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000960426.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser",
          "transcript": "ENST00000680748.1",
          "protein_id": "ENSP00000506141.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 97,
          "cds_start": 47,
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          "cdna_start": 302,
          "cdna_end": null,
          "cdna_length": 763,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000680748.1"
        },
        {
          "aa_ref": null,
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          "protein_coding": true,
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            "intron_variant"
          ],
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          "exon_count": 4,
          "intron_rank": 2,
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          "gene_symbol": "ATF4",
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          "hgvs_c": "c.30+17T>C",
          "hgvs_p": null,
          "transcript": "ENST00000675582.2",
          "protein_id": "ENSP00000502056.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 322,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 969,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1249,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675582.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ATF4",
          "gene_hgnc_id": 786,
          "hgvs_c": "c.-26-281T>C",
          "hgvs_p": null,
          "transcript": "ENST00000680446.1",
          "protein_id": "ENSP00000506657.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 804,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1004,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680446.1"
        }
      ],
      "gene_symbol": "ATF4",
      "gene_hgnc_id": 786,
      "dbsnp": "rs1930940865",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.13624009490013123,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.041,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1252,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.5,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.139,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001675.4",
          "gene_symbol": "ATF4",
          "hgnc_id": 786,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.47T>C",
          "hgvs_p": "p.Leu16Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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