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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-41583996-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=41583996&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 41583996,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_002676.3",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "NM_002676.3",
          "protein_id": "NP_002667.2",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000216259.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002676.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "ENST00000216259.8",
          "protein_id": "ENSP00000216259.7",
          "transcript_support_level": 1,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002676.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000216259.8"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.671C>A",
          "hgvs_p": "p.Thr224Asn",
          "transcript": "ENST00000949539.1",
          "protein_id": "ENSP00000619598.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949539.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.566C>A",
          "hgvs_p": "p.Thr189Asn",
          "transcript": "ENST00000940470.1",
          "protein_id": "ENSP00000610529.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940470.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.482C>A",
          "hgvs_p": "p.Thr161Asn",
          "transcript": "ENST00000949538.1",
          "protein_id": "ENSP00000619597.1",
          "transcript_support_level": null,
          "aa_start": 161,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 482,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949538.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "ENST00000949537.1",
          "protein_id": "ENSP00000619596.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949537.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.458C>A",
          "hgvs_p": "p.Thr153Asn",
          "transcript": "ENST00000896620.1",
          "protein_id": "ENSP00000566679.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 458,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896620.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "ENST00000949536.1",
          "protein_id": "ENSP00000619595.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949536.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "ENST00000940468.1",
          "protein_id": "ENSP00000610527.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 261,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 786,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940468.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "ENST00000940469.1",
          "protein_id": "ENSP00000610528.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000940469.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.431C>A",
          "hgvs_p": "p.Thr144Asn",
          "transcript": "ENST00000940471.1",
          "protein_id": "ENSP00000610530.1",
          "transcript_support_level": null,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 431,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940471.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
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          "hgvs_c": "c.428C>A",
          "hgvs_p": "p.Thr143Asn",
          "transcript": "ENST00000896615.1",
          "protein_id": "ENSP00000566674.1",
          "transcript_support_level": null,
          "aa_start": 143,
          "aa_end": null,
          "aa_length": 259,
          "cds_start": 428,
          "cds_end": null,
          "cds_length": 780,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896615.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.404C>A",
          "hgvs_p": "p.Thr135Asn",
          "transcript": "ENST00000896617.1",
          "protein_id": "ENSP00000566676.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 251,
          "cds_start": 404,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000896617.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn",
          "transcript": "ENST00000896616.1",
          "protein_id": "ENSP00000566675.1",
          "transcript_support_level": null,
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          "cds_start": 437,
          "cds_end": null,
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        },
        {
          "aa_ref": "H",
          "aa_alt": "Q",
          "canonical": false,
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          "strand": false,
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          ],
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          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.345C>A",
          "hgvs_p": "p.His115Gln",
          "transcript": "ENST00000896618.1",
          "protein_id": "ENSP00000566677.1",
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          "aa_start": 115,
          "aa_end": null,
          "aa_length": 206,
          "cds_start": 345,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000896618.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.227C>A",
          "hgvs_p": "p.Thr76Asn",
          "transcript": "ENST00000896619.1",
          "protein_id": "ENSP00000566678.1",
          "transcript_support_level": null,
          "aa_start": 76,
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          "aa_length": 192,
          "cds_start": 227,
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          "cds_length": 579,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.566C>A",
          "hgvs_p": "p.Thr189Asn",
          "transcript": "XM_011530229.3",
          "protein_id": "XP_011528531.1",
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          "aa_start": 189,
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        {
          "aa_ref": "T",
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          ],
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "PMM1",
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          "hgvs_c": "c.452C>A",
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          "transcript": "XM_011530230.3",
          "protein_id": "XP_011528532.1",
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          "cds_start": 452,
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          "cdna_length": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "T",
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          "strand": false,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.227C>A",
          "hgvs_p": "p.Thr76Asn",
          "transcript": "XM_011530231.4",
          "protein_id": "XP_011528533.1",
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          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011530231.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.146C>A",
          "hgvs_p": "p.Thr49Asn",
          "transcript": "XM_005261638.5",
          "protein_id": "XP_005261695.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 165,
          "cds_start": 146,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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        {
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          "gene_symbol": "PMM1",
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          "hgvs_c": "n.672C>A",
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        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 6,
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          "gene_symbol": "PMM1",
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          "transcript": "ENST00000414636.1",
          "protein_id": "ENSP00000400889.1",
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          "cdna_length": null,
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          "biotype": "nonsense_mediated_decay",
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        {
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          "protein_coding": false,
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          "consequences": [
            "downstream_gene_variant"
          ],
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          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "PMM1",
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          "hgvs_c": "n.*10C>A",
          "hgvs_p": null,
          "transcript": "ENST00000478337.1",
          "protein_id": null,
          "transcript_support_level": 2,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000478337.1"
        }
      ],
      "gene_symbol": "PMM1",
      "gene_hgnc_id": 9114,
      "dbsnp": "rs749651441",
      "frequency_reference_population": 0.0000049573423,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 8,
      "gnomad_exomes_af": 0.00000273676,
      "gnomad_genomes_af": 0.000026284,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8296580910682678,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.651,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1758,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.08,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 6.512,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002676.3",
          "gene_symbol": "PMM1",
          "hgnc_id": 9114,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.437C>A",
          "hgvs_p": "p.Thr146Asn"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}