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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-42627674-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=42627674&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 42627674,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "ENST00000352397.10",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.478C>T",
          "hgvs_p": "p.Arg160*",
          "transcript": "NM_000398.7",
          "protein_id": "NP_000389.1",
          "transcript_support_level": null,
          "aa_start": 160,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 478,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": 555,
          "cdna_end": null,
          "cdna_length": 2916,
          "mane_select": "ENST00000352397.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.478C>T",
          "hgvs_p": "p.Arg160*",
          "transcript": "ENST00000352397.10",
          "protein_id": "ENSP00000338461.6",
          "transcript_support_level": 1,
          "aa_start": 160,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 478,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": 555,
          "cdna_end": null,
          "cdna_length": 2916,
          "mane_select": "NM_000398.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.496C>T",
          "hgvs_p": "p.Arg166*",
          "transcript": "ENST00000407332.6",
          "protein_id": "ENSP00000384457.2",
          "transcript_support_level": 1,
          "aa_start": 166,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 496,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": 516,
          "cdna_end": null,
          "cdna_length": 1904,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "n.2612C>T",
          "hgvs_p": null,
          "transcript": "ENST00000470741.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3938,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000289517",
          "gene_hgnc_id": null,
          "hgvs_c": "n.13C>T",
          "hgvs_p": null,
          "transcript": "ENST00000617178.5",
          "protein_id": "ENSP00000482500.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.610C>T",
          "hgvs_p": "p.Arg204*",
          "transcript": "ENST00000361740.9",
          "protein_id": "ENSP00000354468.5",
          "transcript_support_level": 2,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": 687,
          "cdna_end": null,
          "cdna_length": 3048,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.577C>T",
          "hgvs_p": "p.Arg193*",
          "transcript": "NM_001171660.2",
          "protein_id": "NP_001165131.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 577,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 694,
          "cdna_end": null,
          "cdna_length": 3055,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.577C>T",
          "hgvs_p": "p.Arg193*",
          "transcript": "ENST00000688117.1",
          "protein_id": "ENSP00000509015.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 577,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 702,
          "cdna_end": null,
          "cdna_length": 2094,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.478C>T",
          "hgvs_p": "p.Arg160*",
          "transcript": "ENST00000693363.1",
          "protein_id": "ENSP00000510411.1",
          "transcript_support_level": null,
          "aa_start": 160,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 478,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": 525,
          "cdna_end": null,
          "cdna_length": 1955,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.478C>T",
          "hgvs_p": "p.Arg160*",
          "transcript": "ENST00000693367.1",
          "protein_id": "ENSP00000508815.1",
          "transcript_support_level": null,
          "aa_start": 160,
          "aa_end": null,
          "aa_length": 298,
          "cds_start": 478,
          "cds_end": null,
          "cds_length": 897,
          "cdna_start": 485,
          "cdna_end": null,
          "cdna_length": 1238,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "NM_001129819.2",
          "protein_id": "NP_001123291.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 806,
          "cdna_end": null,
          "cdna_length": 3167,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "NM_001171661.1",
          "protein_id": "NP_001165132.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 622,
          "cdna_end": null,
          "cdna_length": 2983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "NM_007326.4",
          "protein_id": "NP_015565.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 561,
          "cdna_end": null,
          "cdna_length": 2922,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "ENST00000402438.6",
          "protein_id": "ENSP00000385679.1",
          "transcript_support_level": 5,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 605,
          "cdna_end": null,
          "cdna_length": 1946,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "ENST00000407623.8",
          "protein_id": "ENSP00000384834.3",
          "transcript_support_level": 3,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 794,
          "cdna_end": null,
          "cdna_length": 2149,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "ENST00000687198.1",
          "protein_id": "ENSP00000508492.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 642,
          "cdna_end": null,
          "cdna_length": 2030,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "ENST00000692152.1",
          "protein_id": "ENSP00000509317.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": 739,
          "cdna_end": null,
          "cdna_length": 2127,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.409C>T",
          "hgvs_p": "p.Arg137*",
          "transcript": "ENST00000438270.2",
          "protein_id": "ENSP00000403439.2",
          "transcript_support_level": 3,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 478,
          "cdna_start": 512,
          "cdna_end": null,
          "cdna_length": 581,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.448C>T",
          "hgvs_p": "p.Arg150*",
          "transcript": "XM_047441183.1",
          "protein_id": "XP_047297139.1",
          "transcript_support_level": null,
          "aa_start": 150,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 448,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": 627,
          "cdna_end": null,
          "cdna_length": 2988,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "n.2218C>T",
          "hgvs_p": null,
          "transcript": "ENST00000684963.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3606,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "n.*427C>T",
          "hgvs_p": null,
          "transcript": "ENST00000686523.1",
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      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 3.894,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
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            "PM2",
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          "verdict": "Pathogenic",
          "transcript": "ENST00000352397.10",
          "gene_symbol": "CYB5R3",
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          "effects": [
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          "hgvs_p": "p.Arg160*"
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        {
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          "verdict": "Pathogenic",
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          "gene_symbol": "ENSG00000289517",
          "hgnc_id": null,
          "effects": [
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          "inheritance_mode": "",
          "hgvs_c": "n.13C>T",
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      ],
      "clinvar_disease": " TYPE II,Deficiency of cytochrome-b5 reductase,METHEMOGLOBINEMIA,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:3",
      "phenotype_combined": "METHEMOGLOBINEMIA, TYPE II|not provided|Deficiency of cytochrome-b5 reductase",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
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  ],
  "message": null
}