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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-42636736-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=42636736&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 42636736,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "NM_001171660.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "NM_000398.7",
          "protein_id": "NP_000389.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000352397.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000398.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000352397.10",
          "protein_id": "ENSP00000338461.6",
          "transcript_support_level": 1,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000398.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000352397.10"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000407332.6",
          "protein_id": "ENSP00000384457.2",
          "transcript_support_level": 1,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000407332.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000361740.9",
          "protein_id": "ENSP00000354468.5",
          "transcript_support_level": 2,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361740.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.231G>A",
          "hgvs_p": "p.Pro77Pro",
          "transcript": "NM_001171660.2",
          "protein_id": "NP_001165131.1",
          "transcript_support_level": null,
          "aa_start": 77,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 231,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001171660.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.231G>A",
          "hgvs_p": "p.Pro77Pro",
          "transcript": "ENST00000688117.1",
          "protein_id": "ENSP00000509015.1",
          "transcript_support_level": null,
          "aa_start": 77,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 231,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000688117.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000693363.1",
          "protein_id": "ENSP00000510411.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000693363.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000966833.1",
          "protein_id": "ENSP00000636892.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 927,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966833.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000693367.1",
          "protein_id": "ENSP00000508815.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 298,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 897,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000693367.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000897015.1",
          "protein_id": "ENSP00000567074.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897015.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000897017.1",
          "protein_id": "ENSP00000567076.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 284,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 855,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897017.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "NM_001129819.2",
          "protein_id": "NP_001123291.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 63,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001129819.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "NM_001171661.1",
          "protein_id": "NP_001165132.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 63,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001171661.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "NM_007326.4",
          "protein_id": "NP_015565.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 63,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_007326.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "ENST00000402438.6",
          "protein_id": "ENSP00000385679.1",
          "transcript_support_level": 5,
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          "aa_end": null,
          "aa_length": 278,
          "cds_start": 63,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000402438.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "ENST00000407623.8",
          "protein_id": "ENSP00000384834.3",
          "transcript_support_level": 3,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 63,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000407623.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "ENST00000687198.1",
          "protein_id": "ENSP00000508492.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 278,
          "cds_start": 63,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.63G>A",
          "hgvs_p": "p.Pro21Pro",
          "transcript": "ENST00000692152.1",
          "protein_id": "ENSP00000509317.1",
          "transcript_support_level": null,
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          "cds_start": 63,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
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          "consequences": [
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          ],
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          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000897016.1",
          "protein_id": "ENSP00000567075.1",
          "transcript_support_level": null,
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          "aa_length": 275,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000897016.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.132G>A",
          "hgvs_p": "p.Pro44Pro",
          "transcript": "ENST00000689195.1",
          "protein_id": "ENSP00000509895.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 132,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "n.111G>A",
          "hgvs_p": null,
          "transcript": "ENST00000693646.1",
          "protein_id": "ENSP00000508449.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000693646.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "n.360G>A",
          "hgvs_p": null,
          "transcript": "ENST00000693716.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000693716.1"
        }
      ],
      "gene_symbol": "CYB5R3",
      "gene_hgnc_id": 2873,
      "dbsnp": "rs5996200",
      "frequency_reference_population": 0.13415882,
      "hom_count_reference_population": 15689,
      "allele_count_reference_population": 216337,
      "gnomad_exomes_af": 0.135124,
      "gnomad_genomes_af": 0.124901,
      "gnomad_exomes_ac": 197319,
      "gnomad_genomes_ac": 19018,
      "gnomad_exomes_homalt": 14379,
      "gnomad_genomes_homalt": 1310,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.33000001311302185,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -2.957,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -19,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6_Very_Strong,BP7,BA1",
      "acmg_by_gene": [
        {
          "score": -19,
          "benign_score": 19,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6_Very_Strong",
            "BP7",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001171660.2",
          "gene_symbol": "CYB5R3",
          "hgnc_id": 2873,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.231G>A",
          "hgvs_p": "p.Pro77Pro"
        }
      ],
      "clinvar_disease": "not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:5",
      "phenotype_combined": "not specified|not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}