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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-42693169-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=42693169&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 42693169,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_017436.7",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "NM_017436.7",
          "protein_id": "NP_059132.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000642412.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017436.7"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000642412.2",
          "protein_id": "ENSP00000494127.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017436.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642412.2"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000249005.3",
          "protein_id": "ENSP00000249005.2",
          "transcript_support_level": 1,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000249005.3"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000401850.5",
          "protein_id": "ENSP00000384794.1",
          "transcript_support_level": 1,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000401850.5"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "NM_001318038.3",
          "protein_id": "NP_001304967.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318038.3"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000381278.4",
          "protein_id": "ENSP00000370678.3",
          "transcript_support_level": 5,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381278.4"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868257.1",
          "protein_id": "ENSP00000538316.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868257.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868258.1",
          "protein_id": "ENSP00000538317.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868258.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868259.1",
          "protein_id": "ENSP00000538318.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868259.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868260.1",
          "protein_id": "ENSP00000538319.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868260.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868261.1",
          "protein_id": "ENSP00000538320.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868261.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868262.1",
          "protein_id": "ENSP00000538321.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
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          "cds_length": 1062,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868263.1",
          "protein_id": "ENSP00000538322.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000868263.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868264.1",
          "protein_id": "ENSP00000538323.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
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        },
        {
          "aa_ref": "W",
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          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868265.1",
          "protein_id": "ENSP00000538324.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
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          "cds_length": 1062,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000868265.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868266.1",
          "protein_id": "ENSP00000538325.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000868266.1"
        },
        {
          "aa_ref": "W",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868267.1",
          "protein_id": "ENSP00000538326.1",
          "transcript_support_level": null,
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          "cds_start": 783,
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        {
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          ],
          "exon_rank": 3,
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          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "A4GALT",
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          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868268.1",
          "protein_id": "ENSP00000538327.1",
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          "aa_start": 261,
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          "cds_start": 783,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
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          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868269.1",
          "protein_id": "ENSP00000538328.1",
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          "cds_start": 783,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000868269.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "ENST00000868270.1",
          "protein_id": "ENSP00000538329.1",
          "transcript_support_level": null,
          "aa_start": 261,
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          "cds_start": 783,
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          "cds_length": 1062,
          "cdna_start": null,
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          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "XM_017028831.2",
          "protein_id": "XP_016884320.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017028831.2"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "A4GALT",
          "gene_hgnc_id": 18149,
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys",
          "transcript": "XM_047441412.1",
          "protein_id": "XP_047297368.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 783,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047441412.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYB5R3",
          "gene_hgnc_id": 2873,
          "hgvs_c": "c.-49+27628G>C",
          "hgvs_p": null,
          "transcript": "ENST00000686129.1",
          "protein_id": "ENSP00000508623.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 130,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 394,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000686129.1"
        }
      ],
      "gene_symbol": "A4GALT",
      "gene_hgnc_id": 18149,
      "dbsnp": "rs74315454",
      "frequency_reference_population": 0.0000037557227,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 6,
      "gnomad_exomes_af": 0.00000207562,
      "gnomad_genomes_af": 0.0000197096,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8809890747070312,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.682,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9342,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.19,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 3.779,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_017436.7",
          "gene_symbol": "A4GALT",
          "hgnc_id": 18149,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "BG",
          "hgvs_c": "c.783G>C",
          "hgvs_p": "p.Trp261Cys"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000686129.1",
          "gene_symbol": "CYB5R3",
          "hgnc_id": 2873,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-49+27628G>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}