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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-50217771-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=50217771&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 50217771,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_020461.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5425C>T",
          "hgvs_p": "p.Arg1809Cys",
          "transcript": "NM_020461.4",
          "protein_id": "NP_065194.3",
          "transcript_support_level": null,
          "aa_start": 1809,
          "aa_end": null,
          "aa_length": 1819,
          "cds_start": 5425,
          "cds_end": null,
          "cds_length": 5460,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000248846.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020461.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5425C>T",
          "hgvs_p": "p.Arg1809Cys",
          "transcript": "ENST00000248846.10",
          "protein_id": "ENSP00000248846.5",
          "transcript_support_level": 1,
          "aa_start": 1809,
          "aa_end": null,
          "aa_length": 1819,
          "cds_start": 5425,
          "cds_end": null,
          "cds_length": 5460,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020461.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000248846.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.1432C>T",
          "hgvs_p": "p.Arg478Cys",
          "transcript": "ENST00000425018.1",
          "protein_id": "ENSP00000405979.1",
          "transcript_support_level": 1,
          "aa_start": 478,
          "aa_end": null,
          "aa_length": 488,
          "cds_start": 1432,
          "cds_end": null,
          "cds_length": 1467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000425018.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "n.*1002C>T",
          "hgvs_p": null,
          "transcript": "ENST00000439308.7",
          "protein_id": "ENSP00000397387.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000439308.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "n.5192C>T",
          "hgvs_p": null,
          "transcript": "ENST00000498611.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000498611.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "n.*1002C>T",
          "hgvs_p": null,
          "transcript": "ENST00000439308.7",
          "protein_id": "ENSP00000397387.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000439308.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5413C>T",
          "hgvs_p": "p.Arg1805Cys",
          "transcript": "ENST00000949023.1",
          "protein_id": "ENSP00000619082.1",
          "transcript_support_level": null,
          "aa_start": 1805,
          "aa_end": null,
          "aa_length": 1815,
          "cds_start": 5413,
          "cds_end": null,
          "cds_length": 5448,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949023.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5389C>T",
          "hgvs_p": "p.Arg1797Cys",
          "transcript": "ENST00000949026.1",
          "protein_id": "ENSP00000619085.1",
          "transcript_support_level": null,
          "aa_start": 1797,
          "aa_end": null,
          "aa_length": 1807,
          "cds_start": 5389,
          "cds_end": null,
          "cds_length": 5424,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949026.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5377C>T",
          "hgvs_p": "p.Arg1793Cys",
          "transcript": "ENST00000883330.1",
          "protein_id": "ENSP00000553389.1",
          "transcript_support_level": null,
          "aa_start": 1793,
          "aa_end": null,
          "aa_length": 1803,
          "cds_start": 5377,
          "cds_end": null,
          "cds_length": 5412,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883330.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5347C>T",
          "hgvs_p": "p.Arg1783Cys",
          "transcript": "ENST00000949024.1",
          "protein_id": "ENSP00000619083.1",
          "transcript_support_level": null,
          "aa_start": 1783,
          "aa_end": null,
          "aa_length": 1793,
          "cds_start": 5347,
          "cds_end": null,
          "cds_length": 5382,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949024.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.5335C>T",
          "hgvs_p": "p.Arg1779Cys",
          "transcript": "ENST00000949028.1",
          "protein_id": "ENSP00000619087.1",
          "transcript_support_level": null,
          "aa_start": 1779,
          "aa_end": null,
          "aa_length": 1789,
          "cds_start": 5335,
          "cds_end": null,
          "cds_length": 5370,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949028.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.4729C>T",
          "hgvs_p": "p.Arg1577Cys",
          "transcript": "ENST00000949025.1",
          "protein_id": "ENSP00000619084.1",
          "transcript_support_level": null,
          "aa_start": 1577,
          "aa_end": null,
          "aa_length": 1587,
          "cds_start": 4729,
          "cds_end": null,
          "cds_length": 4764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949025.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "c.3730C>T",
          "hgvs_p": "p.Arg1244Cys",
          "transcript": "ENST00000949027.1",
          "protein_id": "ENSP00000619086.1",
          "transcript_support_level": null,
          "aa_start": 1244,
          "aa_end": null,
          "aa_length": 1254,
          "cds_start": 3730,
          "cds_end": null,
          "cds_length": 3765,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949027.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TUBGCP6",
          "gene_hgnc_id": 18127,
          "hgvs_c": "n.3967C>T",
          "hgvs_p": null,
          "transcript": "ENST00000491449.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000491449.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SELENOO",
          "gene_hgnc_id": 30395,
          "hgvs_c": "c.*402G>A",
          "hgvs_p": null,
          "transcript": "NM_031454.2",
          "protein_id": "NP_113642.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 669,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000380903.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031454.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SELENOO",
          "gene_hgnc_id": 30395,
          "hgvs_c": "c.*402G>A",
          "hgvs_p": null,
          "transcript": "ENST00000380903.7",
          "protein_id": "ENSP00000370288.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 669,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_031454.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380903.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SELENOO",
          "gene_hgnc_id": 30395,
          "hgvs_c": "n.*155G>A",
          "hgvs_p": null,
          "transcript": "ENST00000492092.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000492092.1"
        }
      ],
      "gene_symbol": "TUBGCP6",
      "gene_hgnc_id": 18127,
      "dbsnp": "rs1236441479",
      "frequency_reference_population": 0.000008674607,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 14,
      "gnomad_exomes_af": 0.00000889332,
      "gnomad_genomes_af": 0.00000657315,
      "gnomad_exomes_ac": 13,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8535690307617188,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.355,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.785,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.3,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.604,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_020461.4",
          "gene_symbol": "TUBGCP6",
          "hgnc_id": 18127,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.5425C>T",
          "hgvs_p": "p.Arg1809Cys"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_031454.2",
          "gene_symbol": "SELENOO",
          "hgnc_id": 30395,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*402G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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