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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-50443885-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=50443885&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 50443885,
      "ref": "C",
      "alt": "A",
      "effect": "synonymous_variant",
      "transcript": "NM_001365836.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2599C>A",
          "hgvs_p": "p.Arg867Arg",
          "transcript": "NM_001242898.2",
          "protein_id": "NP_001229827.1",
          "transcript_support_level": null,
          "aa_start": 867,
          "aa_end": null,
          "aa_length": 959,
          "cds_start": 2599,
          "cds_end": null,
          "cds_length": 2880,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000612753.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001242898.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2599C>A",
          "hgvs_p": "p.Arg867Arg",
          "transcript": "ENST00000612753.5",
          "protein_id": "ENSP00000478417.1",
          "transcript_support_level": 2,
          "aa_start": 867,
          "aa_end": null,
          "aa_length": 959,
          "cds_start": 2599,
          "cds_end": null,
          "cds_length": 2880,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001242898.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000612753.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "ENST00000216061.9",
          "protein_id": "ENSP00000216061.5",
          "transcript_support_level": 1,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2620,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000216061.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2521C>A",
          "hgvs_p": "p.Arg841Arg",
          "transcript": "ENST00000395741.7",
          "protein_id": "ENSP00000379090.3",
          "transcript_support_level": 1,
          "aa_start": 841,
          "aa_end": null,
          "aa_length": 933,
          "cds_start": 2521,
          "cds_end": null,
          "cds_length": 2802,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395741.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2518C>A",
          "hgvs_p": "p.Arg840Arg",
          "transcript": "ENST00000395744.7",
          "protein_id": "ENSP00000379093.3",
          "transcript_support_level": 1,
          "aa_start": 840,
          "aa_end": null,
          "aa_length": 932,
          "cds_start": 2518,
          "cds_end": null,
          "cds_length": 2799,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395744.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2629C>A",
          "hgvs_p": "p.Arg877Arg",
          "transcript": "ENST00000906623.1",
          "protein_id": "ENSP00000576682.1",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 969,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2910,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906623.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2623C>A",
          "hgvs_p": "p.Arg875Arg",
          "transcript": "ENST00000906628.1",
          "protein_id": "ENSP00000576687.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 967,
          "cds_start": 2623,
          "cds_end": null,
          "cds_length": 2904,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906628.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2623C>A",
          "hgvs_p": "p.Arg875Arg",
          "transcript": "ENST00000938142.1",
          "protein_id": "ENSP00000608201.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 967,
          "cds_start": 2623,
          "cds_end": null,
          "cds_length": 2904,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000938142.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2623C>A",
          "hgvs_p": "p.Arg875Arg",
          "transcript": "ENST00000966967.1",
          "protein_id": "ENSP00000637026.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 967,
          "cds_start": 2623,
          "cds_end": null,
          "cds_length": 2904,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966967.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "NM_001365836.1",
          "protein_id": "NP_001352765.1",
          "transcript_support_level": null,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2620,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001365836.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "ENST00000906618.1",
          "protein_id": "ENSP00000576677.1",
          "transcript_support_level": null,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2620,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906618.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "ENST00000906645.1",
          "protein_id": "ENSP00000576704.1",
          "transcript_support_level": null,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2620,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906645.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "ENST00000906649.1",
          "protein_id": "ENSP00000576708.1",
          "transcript_support_level": null,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2620,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906649.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "ENST00000966975.1",
          "protein_id": "ENSP00000637034.1",
          "transcript_support_level": null,
          "aa_start": 874,
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          "cds_start": 2620,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000966975.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg",
          "transcript": "ENST00000966986.1",
          "protein_id": "ENSP00000637045.1",
          "transcript_support_level": null,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2620,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966986.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2605C>A",
          "hgvs_p": "p.Arg869Arg",
          "transcript": "ENST00000906621.1",
          "protein_id": "ENSP00000576680.1",
          "transcript_support_level": null,
          "aa_start": 869,
          "aa_end": null,
          "aa_length": 961,
          "cds_start": 2605,
          "cds_end": null,
          "cds_length": 2886,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906621.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2602C>A",
          "hgvs_p": "p.Arg868Arg",
          "transcript": "NM_001351641.2",
          "protein_id": "NP_001338570.1",
          "transcript_support_level": null,
          "aa_start": 868,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2602C>A",
          "hgvs_p": "p.Arg868Arg",
          "transcript": "NM_001351642.2",
          "protein_id": "NP_001338571.1",
          "transcript_support_level": null,
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          "aa_length": 960,
          "cds_start": 2602,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "NM_001351642.2"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
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          "exon_count": 24,
          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2602C>A",
          "hgvs_p": "p.Arg868Arg",
          "transcript": "ENST00000906614.1",
          "protein_id": "ENSP00000576673.1",
          "transcript_support_level": null,
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          "aa_length": 960,
          "cds_start": 2602,
          "cds_end": null,
          "cds_length": 2883,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906614.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2602C>A",
          "hgvs_p": "p.Arg868Arg",
          "transcript": "ENST00000906617.1",
          "protein_id": "ENSP00000576676.1",
          "transcript_support_level": null,
          "aa_start": 868,
          "aa_end": null,
          "aa_length": 960,
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        {
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          "exon_count": 22,
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        {
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          "protein_coding": false,
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          "consequences": [
            "non_coding_transcript_exon_variant"
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          "exon_rank": 1,
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          "exon_count": 2,
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          "gene_symbol": "PPP6R2",
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          "hgvs_c": "n.799C>A",
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          "transcript": "ENST00000470046.1",
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          "transcript_support_level": 2,
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          "mane_select": null,
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          "biotype": "retained_intron",
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        {
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          "protein_coding": false,
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          "consequences": [
            "non_coding_transcript_exon_variant"
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          "exon_rank": 9,
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          "exon_count": 9,
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          "gene_symbol": "SBF1",
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          "transcript": "ENST00000685180.1",
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          "biotype": "retained_intron",
          "feature": "ENST00000685180.1"
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      ],
      "gene_symbol": "PPP6R2",
      "gene_hgnc_id": 19253,
      "dbsnp": "rs200415574",
      "frequency_reference_population": 0.0000027943888,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000279439,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.800000011920929,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.8,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.222,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP7",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 5,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001365836.1",
          "gene_symbol": "PPP6R2",
          "hgnc_id": 19253,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2620C>A",
          "hgvs_p": "p.Arg874Arg"
        },
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000685180.1",
          "gene_symbol": "SBF1",
          "hgnc_id": 10542,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "n.6631G>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}