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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-108351844-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=108351844&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 108351844,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_007072.4",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "NM_001282556.2",
          "protein_id": "NP_001269485.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000467761.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001282556.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000467761.6",
          "protein_id": "ENSP00000419207.1",
          "transcript_support_level": 5,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001282556.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000467761.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000357759.9",
          "protein_id": "ENSP00000350402.5",
          "transcript_support_level": 1,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000357759.9"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000489514.6",
          "protein_id": "ENSP00000417856.2",
          "transcript_support_level": 1,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000489514.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000491820.5",
          "protein_id": "ENSP00000418284.1",
          "transcript_support_level": 1,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000491820.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896079.1",
          "protein_id": "ENSP00000566138.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896079.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896086.1",
          "protein_id": "ENSP00000566145.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896086.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "NM_001282557.2",
          "protein_id": "NP_001269486.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001282557.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "NM_001370244.1",
          "protein_id": "NP_001357173.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001370244.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "NM_007072.4",
          "protein_id": "NP_009003.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_007072.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896068.1",
          "protein_id": "ENSP00000566127.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896068.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896069.1",
          "protein_id": "ENSP00000566128.1",
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          "aa_start": 11,
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          "aa_length": 414,
          "cds_start": 31,
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          "cds_length": 1245,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000896069.1"
        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896070.1",
          "protein_id": "ENSP00000566129.1",
          "transcript_support_level": null,
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          "cds_start": 31,
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          "cds_length": 1245,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896070.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896071.1",
          "protein_id": "ENSP00000566130.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
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          "cds_start": 31,
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          "cdna_start": null,
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896072.1",
          "protein_id": "ENSP00000566131.1",
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          "cds_start": 31,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000896072.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896075.1",
          "protein_id": "ENSP00000566134.1",
          "transcript_support_level": null,
          "aa_start": 11,
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          "cds_start": 31,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "L",
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          "canonical": false,
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          "strand": true,
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
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          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896076.1",
          "protein_id": "ENSP00000566135.1",
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        {
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          "exon_count": 12,
          "intron_rank": null,
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          "gene_symbol": "HHLA2",
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          "hgvs_c": "c.31C>T",
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          "transcript": "ENST00000896077.1",
          "protein_id": "ENSP00000566136.1",
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "HHLA2",
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          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896078.1",
          "protein_id": "ENSP00000566137.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000896078.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HHLA2",
          "gene_hgnc_id": 4905,
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe",
          "transcript": "ENST00000896080.1",
          "protein_id": "ENSP00000566139.1",
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          "cdna_start": null,
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          "cdna_length": null,
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        {
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          "gene_symbol": "HHLA2",
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          "hgvs_c": "c.-35-1583C>T",
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          "transcript": "ENST00000467282.5",
          "protein_id": "ENSP00000420622.1",
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          "feature": "ENST00000467282.5"
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      ],
      "gene_symbol": "HHLA2",
      "gene_hgnc_id": 4905,
      "dbsnp": "rs775538346",
      "frequency_reference_population": 0.000008062725,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 13,
      "gnomad_exomes_af": 0.00000547865,
      "gnomad_genomes_af": 0.0000328636,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09742361307144165,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.037,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.059,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.48,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.923,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Moderate,BP6_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate",
            "BP6_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_007072.4",
          "gene_symbol": "HHLA2",
          "hgnc_id": 4905,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.31C>T",
          "hgvs_p": "p.Leu11Phe"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}