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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-120644405-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=120644405&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 120644405,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_000187.4",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "NM_000187.4",
          "protein_id": "NP_000178.2",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1338,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000283871.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000187.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "ENST00000283871.10",
          "protein_id": "ENSP00000283871.5",
          "transcript_support_level": 1,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1338,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000187.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000283871.10"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "ENST00000898838.1",
          "protein_id": "ENSP00000568897.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 499,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1500,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898838.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "ENST00000898833.1",
          "protein_id": "ENSP00000568892.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898833.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.682C>T",
          "hgvs_p": "p.Pro228Ser",
          "transcript": "ENST00000898830.1",
          "protein_id": "ENSP00000568889.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 682,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898830.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.676C>T",
          "hgvs_p": "p.Pro226Ser",
          "transcript": "ENST00000898840.1",
          "protein_id": "ENSP00000568899.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 676,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898840.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.616C>T",
          "hgvs_p": "p.Pro206Ser",
          "transcript": "ENST00000898835.1",
          "protein_id": "ENSP00000568894.1",
          "transcript_support_level": null,
          "aa_start": 206,
          "aa_end": null,
          "aa_length": 421,
          "cds_start": 616,
          "cds_end": null,
          "cds_length": 1266,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898835.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.610C>T",
          "hgvs_p": "p.Pro204Ser",
          "transcript": "ENST00000898841.1",
          "protein_id": "ENSP00000568900.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898841.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.610C>T",
          "hgvs_p": "p.Pro204Ser",
          "transcript": "ENST00000955340.1",
          "protein_id": "ENSP00000625399.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 610,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955340.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.421C>T",
          "hgvs_p": "p.Pro141Ser",
          "transcript": "ENST00000898844.1",
          "protein_id": "ENSP00000568903.1",
          "transcript_support_level": null,
          "aa_start": 141,
          "aa_end": null,
          "aa_length": 356,
          "cds_start": 421,
          "cds_end": null,
          "cds_length": 1071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898844.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "ENST00000898832.1",
          "protein_id": "ENSP00000568891.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898832.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.682C>T",
          "hgvs_p": "p.Pro228Ser",
          "transcript": "ENST00000898836.1",
          "protein_id": "ENSP00000568895.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 682,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898836.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.106C>T",
          "hgvs_p": "p.Pro36Ser",
          "transcript": "ENST00000494453.1",
          "protein_id": "ENSP00000419163.1",
          "transcript_support_level": 3,
          "aa_start": 36,
          "aa_end": null,
          "aa_length": 144,
          "cds_start": 106,
          "cds_end": null,
          "cds_length": 435,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000494453.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "XM_005247413.3",
          "protein_id": "XP_005247470.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005247413.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.265C>T",
          "hgvs_p": "p.Pro89Ser",
          "transcript": "XM_017006277.3",
          "protein_id": "XP_016861766.1",
          "transcript_support_level": null,
          "aa_start": 89,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": 265,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017006277.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser",
          "transcript": "XM_011512746.3",
          "protein_id": "XP_011511048.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 294,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 885,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011512746.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.*162C>T",
          "hgvs_p": null,
          "transcript": "XM_005247414.6",
          "protein_id": "XP_005247471.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 233,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 702,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005247414.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.549+2568C>T",
          "hgvs_p": null,
          "transcript": "ENST00000898842.1",
          "protein_id": "ENSP00000568901.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898842.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.549+2568C>T",
          "hgvs_p": null,
          "transcript": "ENST00000898831.1",
          "protein_id": "ENSP00000568890.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898831.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "HGD",
          "gene_hgnc_id": 4892,
          "hgvs_c": "c.543+2568C>T",
          "hgvs_p": null,
          "transcript": "ENST00000898834.1",
          "protein_id": "ENSP00000568893.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000898834.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
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      "gene_symbol": "HGD",
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      "dbsnp": "rs28942100",
      "frequency_reference_population": 0.000046465524,
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      "gnomad_exomes_af": 0.0000478852,
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      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.9772851467132568,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.981,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9617,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.57,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.356,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
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      "acmg_score": 19,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP2,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 19,
          "benign_score": 0,
          "pathogenic_score": 19,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP2",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_000187.4",
          "gene_symbol": "HGD",
          "hgnc_id": 4892,
          "effects": [
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          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.688C>T",
          "hgvs_p": "p.Pro230Ser"
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      ],
      "clinvar_disease": "Alkaptonuria",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:3 O:1",
      "phenotype_combined": "Alkaptonuria",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}