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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-122407316-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=122407316&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "FAM162A",
          "hgnc_id": 17865,
          "hgvs_c": "c.299G>A",
          "hgvs_p": "p.Arg100Gln",
          "inheritance_mode": "AR",
          "pathogenic_score": 6,
          "score": 6,
          "transcript": "NM_014367.4",
          "verdict": "Likely_pathogenic"
        }
      ],
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_score": 6,
      "allele_count_reference_population": 8,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.5457,
      "alt": "A",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.17,
      "chr": "3",
      "clinvar_classification": "Uncertain significance",
      "clinvar_disease": "not specified",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "computational_prediction_selected": "Pathogenic",
      "computational_score_selected": 0.9853404760360718,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 154,
          "aa_ref": "R",
          "aa_start": 100,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3052,
          "cdna_start": 383,
          "cds_end": null,
          "cds_length": 465,
          "cds_start": 299,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 5,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "NM_014367.4",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.299G>A",
          "hgvs_p": "p.Arg100Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000477892.5",
          "protein_coding": true,
          "protein_id": "NP_055182.3",
          "strand": true,
          "transcript": "NM_014367.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 154,
          "aa_ref": "R",
          "aa_start": 100,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 3052,
          "cdna_start": 383,
          "cds_end": null,
          "cds_length": 465,
          "cds_start": 299,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 5,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000477892.5",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.299G>A",
          "hgvs_p": "p.Arg100Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_014367.4",
          "protein_coding": true,
          "protein_id": "ENSP00000419088.1",
          "strand": true,
          "transcript": "ENST00000477892.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 160,
          "aa_ref": "R",
          "aa_start": 106,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3121,
          "cdna_start": 456,
          "cds_end": null,
          "cds_length": 483,
          "cds_start": 317,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 6,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000692876.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.317G>A",
          "hgvs_p": "p.Arg106Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000509253.1",
          "strand": true,
          "transcript": "ENST00000692876.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 144,
          "aa_ref": "R",
          "aa_start": 90,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 801,
          "cdna_start": 549,
          "cds_end": null,
          "cds_length": 435,
          "cds_start": 269,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 6,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000232125.9",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.269G>A",
          "hgvs_p": "p.Arg90Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000232125.5",
          "strand": true,
          "transcript": "ENST00000232125.9",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 141,
          "aa_ref": "R",
          "aa_start": 100,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1015,
          "cdna_start": 367,
          "cds_end": null,
          "cds_length": 426,
          "cds_start": 299,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 4,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000469967.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.299G>A",
          "hgvs_p": "p.Arg100Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000419491.1",
          "strand": true,
          "transcript": "ENST00000469967.1",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "K",
          "aa_end": null,
          "aa_length": 65,
          "aa_ref": "E",
          "aa_start": 65,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2788,
          "cdna_start": 277,
          "cds_end": null,
          "cds_length": 198,
          "cds_start": 193,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000688794.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.193G>A",
          "hgvs_p": "p.Glu65Lys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000508673.1",
          "strand": true,
          "transcript": "ENST00000688794.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 64,
          "aa_ref": "R",
          "aa_start": 10,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3022,
          "cdna_start": 357,
          "cds_end": null,
          "cds_length": 195,
          "cds_start": 29,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 5,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000684791.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.29G>A",
          "hgvs_p": "p.Arg10Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000509860.1",
          "strand": true,
          "transcript": "ENST00000684791.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 64,
          "aa_ref": "R",
          "aa_start": 10,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3044,
          "cdna_start": 379,
          "cds_end": null,
          "cds_length": 195,
          "cds_start": 29,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 5,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000686831.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.29G>A",
          "hgvs_p": "p.Arg10Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000508842.1",
          "strand": true,
          "transcript": "ENST00000686831.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 64,
          "aa_ref": "R",
          "aa_start": 10,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2880,
          "cdna_start": 217,
          "cds_end": null,
          "cds_length": 195,
          "cds_start": 29,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000689215.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "c.29G>A",
          "hgvs_p": "p.Arg10Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000510295.1",
          "strand": true,
          "transcript": "ENST00000689215.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3134,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000685650.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.*173G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000510636.1",
          "strand": true,
          "transcript": "ENST00000685650.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 4825,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000686446.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.2160G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000686446.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 5957,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000688631.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.3300G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000688631.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3033,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000688900.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.*173G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000510506.1",
          "strand": true,
          "transcript": "ENST00000688900.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2955,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000693245.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.193G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000510152.1",
          "strand": true,
          "transcript": "ENST00000693245.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3134,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_count": 4,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000685650.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.*173G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000510636.1",
          "strand": true,
          "transcript": "ENST00000685650.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3033,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000688900.1",
          "gene_hgnc_id": 17865,
          "gene_symbol": "FAM162A",
          "hgvs_c": "n.*173G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000510506.1",
          "strand": true,
          "transcript": "ENST00000688900.1",
          "transcript_support_level": null
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs1192359264",
      "effect": "missense_variant",
      "frequency_reference_population": 0.0000054727725,
      "gene_hgnc_id": 17865,
      "gene_symbol": "FAM162A",
      "gnomad_exomes_ac": 8,
      "gnomad_exomes_af": 0.00000547277,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": null,
      "gnomad_genomes_af": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Uncertain significance",
      "phenotype_combined": "not specified",
      "phylop100way_prediction": "Uncertain_significance",
      "phylop100way_score": 6.036,
      "pos": 122407316,
      "ref": "G",
      "revel_prediction": "Uncertain_significance",
      "revel_score": 0.446,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0,
      "transcript": "NM_014367.4"
    }
  ]
}
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