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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-123664189-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=123664189&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 123664189,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000360304.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys",
          "transcript": "NM_053025.4",
          "protein_id": "NP_444253.3",
          "transcript_support_level": null,
          "aa_start": 1301,
          "aa_end": null,
          "aa_length": 1914,
          "cds_start": 3901,
          "cds_end": null,
          "cds_length": 5745,
          "cdna_start": 4213,
          "cdna_end": null,
          "cdna_length": 10113,
          "mane_select": "ENST00000360304.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys",
          "transcript": "ENST00000360304.8",
          "protein_id": "ENSP00000353452.3",
          "transcript_support_level": 5,
          "aa_start": 1301,
          "aa_end": null,
          "aa_length": 1914,
          "cds_start": 3901,
          "cds_end": null,
          "cds_length": 5745,
          "cdna_start": 4213,
          "cdna_end": null,
          "cdna_length": 10113,
          "mane_select": "NM_053025.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "n.*3480C>T",
          "hgvs_p": null,
          "transcript": "ENST00000464489.5",
          "protein_id": "ENSP00000417798.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5901,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "n.*3480C>T",
          "hgvs_p": null,
          "transcript": "ENST00000464489.5",
          "protein_id": "ENSP00000417798.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5901,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3931C>T",
          "hgvs_p": "p.Arg1311Cys",
          "transcript": "ENST00000687848.1",
          "protein_id": "ENSP00000508761.1",
          "transcript_support_level": null,
          "aa_start": 1311,
          "aa_end": null,
          "aa_length": 1924,
          "cds_start": 3931,
          "cds_end": null,
          "cds_length": 5775,
          "cdna_start": 4093,
          "cdna_end": null,
          "cdna_length": 5956,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys",
          "transcript": "ENST00000686761.1",
          "protein_id": "ENSP00000508758.1",
          "transcript_support_level": null,
          "aa_start": 1301,
          "aa_end": null,
          "aa_length": 1914,
          "cds_start": 3901,
          "cds_end": null,
          "cds_length": 5745,
          "cdna_start": 4260,
          "cdna_end": null,
          "cdna_length": 6162,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys",
          "transcript": "ENST00000686406.1",
          "protein_id": "ENSP00000509044.1",
          "transcript_support_level": null,
          "aa_start": 1301,
          "aa_end": null,
          "aa_length": 1913,
          "cds_start": 3901,
          "cds_end": null,
          "cds_length": 5742,
          "cdna_start": 3904,
          "cdna_end": null,
          "cdna_length": 5783,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys",
          "transcript": "NM_053027.4",
          "protein_id": "NP_444255.3",
          "transcript_support_level": null,
          "aa_start": 1301,
          "aa_end": null,
          "aa_length": 1863,
          "cds_start": 3901,
          "cds_end": null,
          "cds_length": 5592,
          "cdna_start": 4213,
          "cdna_end": null,
          "cdna_length": 9960,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys",
          "transcript": "ENST00000360772.7",
          "protein_id": "ENSP00000354004.3",
          "transcript_support_level": 5,
          "aa_start": 1301,
          "aa_end": null,
          "aa_length": 1863,
          "cds_start": 3901,
          "cds_end": null,
          "cds_length": 5592,
          "cdna_start": 4280,
          "cdna_end": null,
          "cdna_length": 10027,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3694C>T",
          "hgvs_p": "p.Arg1232Cys",
          "transcript": "NM_053026.4",
          "protein_id": "NP_444254.3",
          "transcript_support_level": null,
          "aa_start": 1232,
          "aa_end": null,
          "aa_length": 1845,
          "cds_start": 3694,
          "cds_end": null,
          "cds_length": 5538,
          "cdna_start": 4006,
          "cdna_end": null,
          "cdna_length": 9906,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
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          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3694C>T",
          "hgvs_p": "p.Arg1232Cys",
          "transcript": "ENST00000693689.1",
          "protein_id": "ENSP00000510503.1",
          "transcript_support_level": null,
          "aa_start": 1232,
          "aa_end": null,
          "aa_length": 1845,
          "cds_start": 3694,
          "cds_end": null,
          "cds_length": 5538,
          "cdna_start": 3988,
          "cdna_end": null,
          "cdna_length": 7398,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3694C>T",
          "hgvs_p": "p.Arg1232Cys",
          "transcript": "NM_053028.4",
          "protein_id": "NP_444256.3",
          "transcript_support_level": null,
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          "cds_start": 3694,
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          "cds_length": 5385,
          "cdna_start": 4006,
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          "cdna_length": 9753,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
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        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
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          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3694C>T",
          "hgvs_p": "p.Arg1232Cys",
          "transcript": "ENST00000346322.10",
          "protein_id": "ENSP00000320622.6",
          "transcript_support_level": 5,
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          "aa_end": null,
          "aa_length": 1794,
          "cds_start": 3694,
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          "cdna_start": 3813,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
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          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3373C>T",
          "hgvs_p": "p.Arg1125Cys",
          "transcript": "NM_001321309.2",
          "protein_id": "NP_001308238.1",
          "transcript_support_level": null,
          "aa_start": 1125,
          "aa_end": null,
          "aa_length": 1738,
          "cds_start": 3373,
          "cds_end": null,
          "cds_length": 5217,
          "cdna_start": 4005,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "strand": false,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.3139C>T",
          "hgvs_p": "p.Arg1047Cys",
          "transcript": "ENST00000690457.1",
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          "cdna_start": 3236,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.1135C>T",
          "hgvs_p": "p.Arg379Cys",
          "transcript": "ENST00000685021.1",
          "protein_id": "ENSP00000508447.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
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          "cds_length": 2979,
          "cdna_start": 1632,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
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          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.1135C>T",
          "hgvs_p": "p.Arg379Cys",
          "transcript": "ENST00000688024.1",
          "protein_id": "ENSP00000509803.1",
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          "aa_start": 379,
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          "aa_length": 991,
          "cds_start": 1135,
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          "cds_length": 2976,
          "cdna_start": 1452,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.1018C>T",
          "hgvs_p": "p.Arg340Cys",
          "transcript": "ENST00000686245.1",
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        },
        {
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          "protein_coding": true,
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          "exon_count": 16,
          "intron_rank": null,
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          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.1135C>T",
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          "transcript": "ENST00000688223.1",
          "protein_id": "ENSP00000508935.1",
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          "feature": null
        },
        {
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          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK",
          "gene_hgnc_id": 7590,
          "hgvs_c": "c.1438C>T",
          "hgvs_p": "p.Arg480Cys",
          "transcript": "ENST00000692352.1",
          "protein_id": "ENSP00000510737.1",
          "transcript_support_level": null,
          "aa_start": 480,
          "aa_end": null,
          "aa_length": 719,
          "cds_start": 1438,
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          "cds_length": 2160,
          "cdna_start": 1439,
          "cdna_end": null,
          "cdna_length": 4331,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
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          "cdna_length": 814,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MYLK",
      "gene_hgnc_id": 7590,
      "dbsnp": "rs368321325",
      "frequency_reference_population": 0.00007558167,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 122,
      "gnomad_exomes_af": 0.0000738786,
      "gnomad_genomes_af": 0.0000919299,
      "gnomad_exomes_ac": 108,
      "gnomad_genomes_ac": 14,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3694804906845093,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.387,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2998,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 1.623,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000360304.8",
          "gene_symbol": "MYLK",
          "hgnc_id": 7590,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.3901C>T",
          "hgvs_p": "p.Arg1301Cys"
        },
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000715826.1",
          "gene_symbol": "MYLK-AS1",
          "hgnc_id": 42440,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.345-27388G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " familial thoracic 7,Aortic aneurysm,Familial thoracic aortic aneurysm and aortic dissection,Megacystis-microcolon-intestinal hypoperistalsis syndrome 1,not provided,not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:7",
      "phenotype_combined": "Familial thoracic aortic aneurysm and aortic dissection|Aortic aneurysm, familial thoracic 7|not provided|not specified|Megacystis-microcolon-intestinal hypoperistalsis syndrome 1;Aortic aneurysm, familial thoracic 7",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}