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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-124796426-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=124796426&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 124796426,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_002213.5",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Asn552Ser",
          "transcript": "NM_002213.5",
          "protein_id": "NP_002204.2",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 799,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2400,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000296181.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002213.5"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Asn552Ser",
          "transcript": "ENST00000296181.9",
          "protein_id": "ENSP00000296181.4",
          "transcript_support_level": 1,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 799,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2400,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002213.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000296181.9"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1877A>G",
          "hgvs_p": "p.Asn626Ser",
          "transcript": "ENST00000905025.1",
          "protein_id": "ENSP00000575084.1",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 1877,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905025.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1769A>G",
          "hgvs_p": "p.Asn590Ser",
          "transcript": "ENST00000965613.1",
          "protein_id": "ENSP00000635672.1",
          "transcript_support_level": null,
          "aa_start": 590,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 1769,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965613.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1700A>G",
          "hgvs_p": "p.Asn567Ser",
          "transcript": "ENST00000965616.1",
          "protein_id": "ENSP00000635675.1",
          "transcript_support_level": null,
          "aa_start": 567,
          "aa_end": null,
          "aa_length": 814,
          "cds_start": 1700,
          "cds_end": null,
          "cds_length": 2445,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965616.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1652A>G",
          "hgvs_p": "p.Asn551Ser",
          "transcript": "ENST00000935896.1",
          "protein_id": "ENSP00000605955.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 798,
          "cds_start": 1652,
          "cds_end": null,
          "cds_length": 2397,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935896.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Asn552Ser",
          "transcript": "ENST00000935903.1",
          "protein_id": "ENSP00000605962.1",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 797,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2394,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935903.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1649A>G",
          "hgvs_p": "p.Asn550Ser",
          "transcript": "ENST00000965615.1",
          "protein_id": "ENSP00000635674.1",
          "transcript_support_level": null,
          "aa_start": 550,
          "aa_end": null,
          "aa_length": 797,
          "cds_start": 1649,
          "cds_end": null,
          "cds_length": 2394,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965615.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1631A>G",
          "hgvs_p": "p.Asn544Ser",
          "transcript": "ENST00000935905.1",
          "protein_id": "ENSP00000605964.1",
          "transcript_support_level": null,
          "aa_start": 544,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1631,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935905.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1559A>G",
          "hgvs_p": "p.Asn520Ser",
          "transcript": "ENST00000935898.1",
          "protein_id": "ENSP00000605957.1",
          "transcript_support_level": null,
          "aa_start": 520,
          "aa_end": null,
          "aa_length": 767,
          "cds_start": 1559,
          "cds_end": null,
          "cds_length": 2304,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935898.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Asn552Ser",
          "transcript": "ENST00000965612.1",
          "protein_id": "ENSP00000635671.1",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965612.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1493A>G",
          "hgvs_p": "p.Asn498Ser",
          "transcript": "ENST00000935909.1",
          "protein_id": "ENSP00000605968.1",
          "transcript_support_level": null,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 745,
          "cds_start": 1493,
          "cds_end": null,
          "cds_length": 2238,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935909.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1487A>G",
          "hgvs_p": "p.Asn496Ser",
          "transcript": "ENST00000935906.1",
          "protein_id": "ENSP00000605965.1",
          "transcript_support_level": null,
          "aa_start": 496,
          "aa_end": null,
          "aa_length": 743,
          "cds_start": 1487,
          "cds_end": null,
          "cds_length": 2232,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935906.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1469A>G",
          "hgvs_p": "p.Asn490Ser",
          "transcript": "ENST00000905026.1",
          "protein_id": "ENSP00000575085.1",
          "transcript_support_level": null,
          "aa_start": 490,
          "aa_end": null,
          "aa_length": 737,
          "cds_start": 1469,
          "cds_end": null,
          "cds_length": 2214,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905026.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1430A>G",
          "hgvs_p": "p.Asn477Ser",
          "transcript": "ENST00000935895.1",
          "protein_id": "ENSP00000605954.1",
          "transcript_support_level": null,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1430,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935895.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Asn552Ser",
          "transcript": "ENST00000965617.1",
          "protein_id": "ENSP00000635676.1",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965617.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1424A>G",
          "hgvs_p": "p.Asn475Ser",
          "transcript": "ENST00000935908.1",
          "protein_id": "ENSP00000605967.1",
          "transcript_support_level": null,
          "aa_start": 475,
          "aa_end": null,
          "aa_length": 722,
          "cds_start": 1424,
          "cds_end": null,
          "cds_length": 2169,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935908.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1397A>G",
          "hgvs_p": "p.Asn466Ser",
          "transcript": "ENST00000965614.1",
          "protein_id": "ENSP00000635673.1",
          "transcript_support_level": null,
          "aa_start": 466,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 1397,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965614.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1364A>G",
          "hgvs_p": "p.Asn455Ser",
          "transcript": "ENST00000935902.1",
          "protein_id": "ENSP00000605961.1",
          "transcript_support_level": null,
          "aa_start": 455,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1364,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935902.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGB5",
          "gene_hgnc_id": 6160,
          "hgvs_c": "c.1334A>G",
          "hgvs_p": "p.Asn445Ser",
          "transcript": "ENST00000935900.1",
          "protein_id": "ENSP00000605959.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 692,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 2079,
          "cdna_start": null,
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      "splice_source_selected": "max_spliceai",
      "revel_score": 0.337,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1001,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.27,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.018,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002213.5",
          "gene_symbol": "ITGB5",
          "hgnc_id": 6160,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Asn552Ser"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_007096043.1",
          "gene_symbol": "LOC124909422",
          "hgnc_id": null,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-51A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}