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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-12600233-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=12600233&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 12600233,
      "ref": "T",
      "alt": "G",
      "effect": "synonymous_variant",
      "transcript": "ENST00000251849.9",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.909A>C",
          "hgvs_p": "p.Thr303Thr",
          "transcript": "NM_002880.4",
          "protein_id": "NP_002871.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 909,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": 1240,
          "cdna_end": null,
          "cdna_length": 3191,
          "mane_select": "ENST00000251849.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.909A>C",
          "hgvs_p": "p.Thr303Thr",
          "transcript": "ENST00000251849.9",
          "protein_id": "ENSP00000251849.4",
          "transcript_support_level": 1,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 909,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": 1240,
          "cdna_end": null,
          "cdna_length": 3191,
          "mane_select": "NM_002880.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.969A>C",
          "hgvs_p": "p.Thr323Thr",
          "transcript": "NM_001354689.3",
          "protein_id": "NP_001341618.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 668,
          "cds_start": 969,
          "cds_end": null,
          "cds_length": 2007,
          "cdna_start": 1300,
          "cdna_end": null,
          "cdna_length": 3251,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.969A>C",
          "hgvs_p": "p.Thr323Thr",
          "transcript": "ENST00000442415.7",
          "protein_id": "ENSP00000401888.2",
          "transcript_support_level": 5,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 668,
          "cds_start": 969,
          "cds_end": null,
          "cds_length": 2007,
          "cdna_start": 1300,
          "cdna_end": null,
          "cdna_length": 3251,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.909A>C",
          "hgvs_p": "p.Thr303Thr",
          "transcript": "NM_001354690.3",
          "protein_id": "NP_001341619.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 909,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": 1075,
          "cdna_end": null,
          "cdna_length": 3026,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.909A>C",
          "hgvs_p": "p.Thr303Thr",
          "transcript": "ENST00000685653.1",
          "protein_id": "ENSP00000509968.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 909,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": 2614,
          "cdna_end": null,
          "cdna_length": 4402,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.909A>C",
          "hgvs_p": "p.Thr303Thr",
          "transcript": "ENST00000691899.1",
          "protein_id": "ENSP00000508763.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 909,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": 1075,
          "cdna_end": null,
          "cdna_length": 2983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.897A>C",
          "hgvs_p": "p.Thr299Thr",
          "transcript": "ENST00000690460.1",
          "protein_id": "ENSP00000509106.1",
          "transcript_support_level": null,
          "aa_start": 299,
          "aa_end": null,
          "aa_length": 644,
          "cds_start": 897,
          "cds_end": null,
          "cds_length": 1935,
          "cdna_start": 1228,
          "cdna_end": null,
          "cdna_length": 3141,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.810A>C",
          "hgvs_p": "p.Thr270Thr",
          "transcript": "NM_001354693.3",
          "protein_id": "NP_001341622.1",
          "transcript_support_level": null,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 810,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": 1141,
          "cdna_end": null,
          "cdna_length": 3092,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.810A>C",
          "hgvs_p": "p.Thr270Thr",
          "transcript": "ENST00000685437.1",
          "protein_id": "ENSP00000508794.1",
          "transcript_support_level": null,
          "aa_start": 270,
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          "aa_length": 615,
          "cds_start": 810,
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          "cds_length": 1848,
          "cdna_start": 1234,
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          "cdna_length": 2866,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
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          "gene_symbol": "RAF1",
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          "hgvs_c": "c.810A>C",
          "hgvs_p": "p.Thr270Thr",
          "transcript": "ENST00000688543.1",
          "protein_id": "ENSP00000509612.1",
          "transcript_support_level": null,
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          "cds_start": 810,
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          "cdna_start": 1211,
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          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 9,
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          "exon_count": 17,
          "intron_rank": null,
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          "gene_symbol": "RAF1",
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        {
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          "strand": false,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "hgvs_c": "c.810A>C",
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          "transcript": "ENST00000687923.1",
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        {
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          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 8,
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          "intron_rank": null,
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          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.798A>C",
          "hgvs_p": "p.Thr266Thr",
          "transcript": "ENST00000690397.1",
          "protein_id": "ENSP00000508730.1",
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        },
        {
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          ],
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        {
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          "canonical": false,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.726A>C",
          "hgvs_p": "p.Thr242Thr",
          "transcript": "NM_001354694.3",
          "protein_id": "NP_001341623.1",
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        {
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          "strand": false,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RAF1",
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          "hgvs_c": "c.684A>C",
          "hgvs_p": "p.Thr228Thr",
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        },
        {
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          "transcript": "NM_001354691.3",
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        {
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          ],
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          "gene_symbol": "RAF1",
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
          "hgvs_c": "c.567A>C",
          "hgvs_p": "p.Thr189Thr",
          "transcript": "NM_001354695.3",
          "protein_id": "NP_001341624.1",
          "transcript_support_level": null,
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          "aa_length": 534,
          "cds_start": 567,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": 1028,
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          "cdna_length": 2979,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAF1",
          "gene_hgnc_id": 9829,
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      "dbsnp": "rs5746219",
      "frequency_reference_population": 0.0005359017,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 865,
      "gnomad_exomes_af": 0.000553393,
      "gnomad_genomes_af": 0.000367908,
      "gnomad_exomes_ac": 809,
      "gnomad_genomes_ac": 56,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.7900000214576721,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.07999999821186066,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
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      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.79,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.816,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.08,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS1",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
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          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000251849.9",
          "gene_symbol": "RAF1",
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          "effects": [
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          "inheritance_mode": "AD",
          "hgvs_c": "c.909A>C",
          "hgvs_p": "p.Thr303Thr"
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      ],
      "clinvar_disease": "Cardiovascular phenotype,LEOPARD syndrome 2,Noonan syndrome 5,Noonan syndrome and Noonan-related syndrome,RAF1-related disorder,RASopathy,not provided,not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "reviewed by expert panel",
      "clinvar_submissions_summary": "US:1 LB:7 B:3",
      "phenotype_combined": "not specified|RASopathy|LEOPARD syndrome 2|Noonan syndrome 5|not provided|Noonan syndrome and Noonan-related syndrome|Cardiovascular phenotype|RAF1-related disorder",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}