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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-12839399-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=12839399&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 12839399,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_000994.4",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "NM_000994.4",
          "protein_id": "NP_000985.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000429711.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000994.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000429711.7",
          "protein_id": "ENSP00000416429.2",
          "transcript_support_level": 1,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000994.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000429711.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000396953.6",
          "protein_id": "ENSP00000380156.2",
          "transcript_support_level": 1,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396953.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000289809",
          "gene_hgnc_id": null,
          "hgvs_c": "c.267+7827C>G",
          "hgvs_p": null,
          "transcript": "ENST00000454887.1",
          "protein_id": "ENSP00000403093.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 163,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 492,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000454887.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.282G>C",
          "hgvs_p": "p.Lys94Asn",
          "transcript": "ENST00000273223.10",
          "protein_id": "ENSP00000339064.5",
          "transcript_support_level": 3,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 153,
          "cds_start": 282,
          "cds_end": null,
          "cds_length": 462,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000273223.10"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "NM_001007073.1",
          "protein_id": "NP_001007074.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001007073.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "NM_001007074.1",
          "protein_id": "NP_001007075.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001007074.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000396957.5",
          "protein_id": "ENSP00000380158.1",
          "transcript_support_level": 2,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396957.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000435983.5",
          "protein_id": "ENSP00000388674.1",
          "transcript_support_level": 2,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000435983.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000865045.1",
          "protein_id": "ENSP00000535104.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865045.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000865046.1",
          "protein_id": "ENSP00000535105.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865046.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935876.1",
          "protein_id": "ENSP00000605935.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935876.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935877.1",
          "protein_id": "ENSP00000605936.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935877.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935878.1",
          "protein_id": "ENSP00000605937.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935878.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935879.1",
          "protein_id": "ENSP00000605938.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935879.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935880.1",
          "protein_id": "ENSP00000605939.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000935880.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935881.1",
          "protein_id": "ENSP00000605940.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935882.1",
          "protein_id": "ENSP00000605941.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
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          "cds_start": 228,
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          "cds_length": 408,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000935882.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 4,
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          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935884.1",
          "protein_id": "ENSP00000605943.1",
          "transcript_support_level": null,
          "aa_start": 76,
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          "aa_length": 135,
          "cds_start": 228,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935884.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL32",
          "gene_hgnc_id": 10336,
          "hgvs_c": "c.228G>C",
          "hgvs_p": "p.Lys76Asn",
          "transcript": "ENST00000935885.1",
          "protein_id": "ENSP00000605944.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": 228,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935885.1"
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      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
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      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.17,
      "bayesdelnoaf_prediction": "Benign",
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      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
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          "criteria": [
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_000994.4",
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          "effects": [
            "missense_variant"
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        {
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000454887.1",
          "gene_symbol": "ENSG00000289809",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}