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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-128910051-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=128910051&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 128910051,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_014049.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1594C>T",
          "hgvs_p": "p.Arg532Trp",
          "transcript": "NM_014049.5",
          "protein_id": "NP_054768.2",
          "transcript_support_level": null,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1594,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000308982.12",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014049.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1594C>T",
          "hgvs_p": "p.Arg532Trp",
          "transcript": "ENST00000308982.12",
          "protein_id": "ENSP00000312618.7",
          "transcript_support_level": 1,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1594,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014049.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000308982.12"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFAP92",
          "gene_hgnc_id": 29231,
          "hgvs_c": "c.*248G>A",
          "hgvs_p": null,
          "transcript": "NM_001394090.1",
          "protein_id": "NP_001381019.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1104,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000645291.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001394090.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFAP92",
          "gene_hgnc_id": 29231,
          "hgvs_c": "c.*248G>A",
          "hgvs_p": null,
          "transcript": "ENST00000645291.3",
          "protein_id": "ENSP00000496592.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1104,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001394090.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000645291.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "n.1127C>T",
          "hgvs_p": null,
          "transcript": "ENST00000511526.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000511526.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1594C>T",
          "hgvs_p": "p.Arg532Trp",
          "transcript": "ENST00000681367.1",
          "protein_id": "ENSP00000505309.1",
          "transcript_support_level": null,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 643,
          "cds_start": 1594,
          "cds_end": null,
          "cds_length": 1932,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000681367.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1688C>T",
          "hgvs_p": "p.Ala563Val",
          "transcript": "ENST00000680636.1",
          "protein_id": "ENSP00000504886.1",
          "transcript_support_level": null,
          "aa_start": 563,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 1688,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680636.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1609C>T",
          "hgvs_p": "p.Arg537Trp",
          "transcript": "ENST00000897730.1",
          "protein_id": "ENSP00000567789.1",
          "transcript_support_level": null,
          "aa_start": 537,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 1609,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897730.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1591C>T",
          "hgvs_p": "p.Arg531Trp",
          "transcript": "ENST00000897729.1",
          "protein_id": "ENSP00000567788.1",
          "transcript_support_level": null,
          "aa_start": 531,
          "aa_end": null,
          "aa_length": 620,
          "cds_start": 1591,
          "cds_end": null,
          "cds_length": 1863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897729.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1588C>T",
          "hgvs_p": "p.Arg530Trp",
          "transcript": "ENST00000959715.1",
          "protein_id": "ENSP00000629774.1",
          "transcript_support_level": null,
          "aa_start": 530,
          "aa_end": null,
          "aa_length": 619,
          "cds_start": 1588,
          "cds_end": null,
          "cds_length": 1860,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959715.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1576C>T",
          "hgvs_p": "p.Arg526Trp",
          "transcript": "ENST00000897731.1",
          "protein_id": "ENSP00000567790.1",
          "transcript_support_level": null,
          "aa_start": 526,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 1576,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897731.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1576C>T",
          "hgvs_p": "p.Arg526Trp",
          "transcript": "ENST00000897732.1",
          "protein_id": "ENSP00000567791.1",
          "transcript_support_level": null,
          "aa_start": 526,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 1576,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897732.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1516C>T",
          "hgvs_p": "p.Arg506Trp",
          "transcript": "ENST00000897734.1",
          "protein_id": "ENSP00000567793.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 595,
          "cds_start": 1516,
          "cds_end": null,
          "cds_length": 1788,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897734.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1498C>T",
          "hgvs_p": "p.Arg500Trp",
          "transcript": "ENST00000926997.1",
          "protein_id": "ENSP00000597056.1",
          "transcript_support_level": null,
          "aa_start": 500,
          "aa_end": null,
          "aa_length": 589,
          "cds_start": 1498,
          "cds_end": null,
          "cds_length": 1770,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926997.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1465C>T",
          "hgvs_p": "p.Arg489Trp",
          "transcript": "ENST00000926996.1",
          "protein_id": "ENSP00000597055.1",
          "transcript_support_level": null,
          "aa_start": 489,
          "aa_end": null,
          "aa_length": 578,
          "cds_start": 1465,
          "cds_end": null,
          "cds_length": 1737,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926996.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1414C>T",
          "hgvs_p": "p.Arg472Trp",
          "transcript": "ENST00000897733.1",
          "protein_id": "ENSP00000567792.1",
          "transcript_support_level": null,
          "aa_start": 472,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 1414,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897733.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1225C>T",
          "hgvs_p": "p.Arg409Trp",
          "transcript": "NM_001410805.1",
          "protein_id": "NP_001397734.1",
          "transcript_support_level": null,
          "aa_start": 409,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 1225,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410805.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1225C>T",
          "hgvs_p": "p.Arg409Trp",
          "transcript": "ENST00000679715.1",
          "protein_id": "ENSP00000506228.1",
          "transcript_support_level": null,
          "aa_start": 409,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 1225,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679715.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAD9",
          "gene_hgnc_id": 21497,
          "hgvs_c": "c.1225C>T",
          "hgvs_p": "p.Arg409Trp",
          "transcript": "XM_024453484.2",
          "protein_id": "XP_024309252.1",
          "transcript_support_level": null,
          "aa_start": 409,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 1225,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024453484.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFAP92",
          "gene_hgnc_id": 29231,
          "hgvs_c": "c.*248G>A",
          "hgvs_p": null,
          "transcript": "NM_001348520.2",
          "protein_id": "NP_001335449.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 813,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2442,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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      "computational_score_selected": 0.96863853931427,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.754,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4325,
      "alphamissense_prediction": "Uncertain_significance",
      "bayesdelnoaf_score": 0.18,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 2.276,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 16,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PM5,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
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          "criteria": [
            "PM2",
            "PM5",
            "PP3_Strong",
            "PP5_Very_Strong"
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          "verdict": "Pathogenic",
          "transcript": "NM_014049.5",
          "gene_symbol": "ACAD9",
          "hgnc_id": 21497,
          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.1594C>T",
          "hgvs_p": "p.Arg532Trp"
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        {
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            "PP3_Strong",
            "PP5_Very_Strong"
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          "verdict": "Pathogenic",
          "transcript": "NM_001394090.1",
          "gene_symbol": "CFAP92",
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          "effects": [
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          ],
          "inheritance_mode": "",
          "hgvs_c": "c.*248G>A",
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      ],
      "clinvar_disease": "Acyl-CoA dehydrogenase 9 deficiency,Mitochondrial complex I deficiency,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:3 LP:1",
      "phenotype_combined": "Acyl-CoA dehydrogenase 9 deficiency|not provided|Mitochondrial complex I deficiency",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}