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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-131029871-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=131029871&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 131029871,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001321221.2",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_024800.5",
          "protein_id": "NP_079076.3",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 645,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 1938,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000383366.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024800.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000383366.9",
          "protein_id": "ENSP00000372857.4",
          "transcript_support_level": 1,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 645,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 1938,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024800.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000383366.9"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000510688.5",
          "protein_id": "ENSP00000423458.1",
          "transcript_support_level": 1,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 599,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 1800,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510688.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000507910.5",
          "protein_id": "ENSP00000426662.1",
          "transcript_support_level": 1,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 482,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 1449,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000507910.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000511262.5",
          "protein_id": "ENSP00000425114.1",
          "transcript_support_level": 1,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 470,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 1413,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000511262.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000972131.1",
          "protein_id": "ENSP00000642190.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 705,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 2118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000972131.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001321221.2",
          "protein_id": "NP_001308150.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 687,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 2064,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321221.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001353022.2",
          "protein_id": "NP_001339951.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 687,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 2064,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353022.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000851275.1",
          "protein_id": "ENSP00000521334.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 687,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 2064,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851275.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000851276.1",
          "protein_id": "ENSP00000521335.1",
          "transcript_support_level": null,
          "aa_start": 55,
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          "aa_length": 687,
          "cds_start": 163,
          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000851276.1"
        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000972124.1",
          "protein_id": "ENSP00000642183.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000972124.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001321220.2",
          "protein_id": "NP_001308149.1",
          "transcript_support_level": null,
          "aa_start": 55,
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          "aa_length": 645,
          "cds_start": 163,
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          "cds_length": 1938,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001321220.2"
        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001353023.2",
          "protein_id": "NP_001339952.1",
          "transcript_support_level": null,
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          "cds_start": 163,
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          "cds_length": 1938,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353023.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000508196.5",
          "protein_id": "ENSP00000421851.1",
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          "cds_start": 163,
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          "cdna_start": null,
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001353024.2",
          "protein_id": "NP_001339953.1",
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          "cds_start": 163,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001353024.2"
        },
        {
          "aa_ref": "E",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001353025.2",
          "protein_id": "NP_001339954.1",
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          "cds_start": 163,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "E",
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          ],
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          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "ENST00000972127.1",
          "protein_id": "ENSP00000642186.1",
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        {
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          "intron_rank": null,
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          "hgvs_c": "c.163G>C",
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        },
        {
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          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
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          "transcript": "NM_001353026.2",
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          "biotype": "protein_coding",
          "feature": "NM_001353026.2"
        },
        {
          "aa_ref": "E",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln",
          "transcript": "NM_001353027.2",
          "protein_id": "NP_001339956.1",
          "transcript_support_level": null,
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          "cds_start": 163,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "pseudogene",
          "feature": "XR_001740271.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK11",
          "gene_hgnc_id": 18593,
          "hgvs_c": "n.*58G>C",
          "hgvs_p": null,
          "transcript": "ENST00000513550.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000513550.1"
        }
      ],
      "gene_symbol": "NEK11",
      "gene_hgnc_id": 18593,
      "dbsnp": "rs776665091",
      "frequency_reference_population": 0.0000020522193,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205222,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3392445743083954,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.182,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1256,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.869,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001321221.2",
          "gene_symbol": "NEK11",
          "hgnc_id": 18593,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.163G>C",
          "hgvs_p": "p.Glu55Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}