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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-134159307-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=134159307&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 134159307,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001005861.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1642C>T",
          "hgvs_p": "p.Pro548Ser",
          "transcript": "NM_002958.4",
          "protein_id": "NP_002949.2",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 607,
          "cds_start": 1642,
          "cds_end": null,
          "cds_length": 1824,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000623711.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002958.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1642C>T",
          "hgvs_p": "p.Pro548Ser",
          "transcript": "ENST00000623711.4",
          "protein_id": "ENSP00000485095.1",
          "transcript_support_level": 1,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 607,
          "cds_start": 1642,
          "cds_end": null,
          "cds_length": 1824,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002958.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000623711.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Pro551Ser",
          "transcript": "ENST00000620660.4",
          "protein_id": "ENSP00000478721.1",
          "transcript_support_level": 1,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1651,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000620660.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "n.4069C>T",
          "hgvs_p": null,
          "transcript": "ENST00000473208.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000473208.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1708C>T",
          "hgvs_p": "p.Pro570Ser",
          "transcript": "ENST00000946535.1",
          "protein_id": "ENSP00000616594.1",
          "transcript_support_level": null,
          "aa_start": 570,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 1708,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946535.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1699C>T",
          "hgvs_p": "p.Pro567Ser",
          "transcript": "ENST00000912304.1",
          "protein_id": "ENSP00000582363.1",
          "transcript_support_level": null,
          "aa_start": 567,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 1699,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912304.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Pro551Ser",
          "transcript": "NM_001005861.3",
          "protein_id": "NP_001005861.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1651,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001005861.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Pro551Ser",
          "transcript": "ENST00000907584.1",
          "protein_id": "ENSP00000577643.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1651,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907584.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Pro551Ser",
          "transcript": "ENST00000912303.1",
          "protein_id": "ENSP00000582362.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1651,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912303.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1642C>T",
          "hgvs_p": "p.Pro548Ser",
          "transcript": "ENST00000907585.1",
          "protein_id": "ENSP00000577644.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 607,
          "cds_start": 1642,
          "cds_end": null,
          "cds_length": 1824,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907585.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1564C>T",
          "hgvs_p": "p.Pro522Ser",
          "transcript": "ENST00000946538.1",
          "protein_id": "ENSP00000616597.1",
          "transcript_support_level": null,
          "aa_start": 522,
          "aa_end": null,
          "aa_length": 581,
          "cds_start": 1564,
          "cds_end": null,
          "cds_length": 1746,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946538.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1555C>T",
          "hgvs_p": "p.Pro519Ser",
          "transcript": "ENST00000912307.1",
          "protein_id": "ENSP00000582366.1",
          "transcript_support_level": null,
          "aa_start": 519,
          "aa_end": null,
          "aa_length": 578,
          "cds_start": 1555,
          "cds_end": null,
          "cds_length": 1737,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912307.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1516C>T",
          "hgvs_p": "p.Pro506Ser",
          "transcript": "ENST00000912305.1",
          "protein_id": "ENSP00000582364.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 565,
          "cds_start": 1516,
          "cds_end": null,
          "cds_length": 1698,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912305.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1486C>T",
          "hgvs_p": "p.Pro496Ser",
          "transcript": "ENST00000946539.1",
          "protein_id": "ENSP00000616598.1",
          "transcript_support_level": null,
          "aa_start": 496,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 1486,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946539.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1462C>T",
          "hgvs_p": "p.Pro488Ser",
          "transcript": "ENST00000946530.1",
          "protein_id": "ENSP00000616589.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 547,
          "cds_start": 1462,
          "cds_end": null,
          "cds_length": 1644,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946530.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1453C>T",
          "hgvs_p": "p.Pro485Ser",
          "transcript": "ENST00000946529.1",
          "protein_id": "ENSP00000616588.1",
          "transcript_support_level": null,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 1453,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946529.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1396C>T",
          "hgvs_p": "p.Pro466Ser",
          "transcript": "ENST00000946532.1",
          "protein_id": "ENSP00000616591.1",
          "transcript_support_level": null,
          "aa_start": 466,
          "aa_end": null,
          "aa_length": 525,
          "cds_start": 1396,
          "cds_end": null,
          "cds_length": 1578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946532.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1342C>T",
          "hgvs_p": "p.Pro448Ser",
          "transcript": "ENST00000946540.1",
          "protein_id": "ENSP00000616599.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 507,
          "cds_start": 1342,
          "cds_end": null,
          "cds_length": 1524,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946540.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1270C>T",
          "hgvs_p": "p.Pro424Ser",
          "transcript": "ENST00000946536.1",
          "protein_id": "ENSP00000616595.1",
          "transcript_support_level": null,
          "aa_start": 424,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 1270,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946536.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYK",
          "gene_hgnc_id": 10481,
          "hgvs_c": "c.1216C>T",
          "hgvs_p": "p.Pro406Ser",
          "transcript": "ENST00000912306.1",
          "protein_id": "ENSP00000582365.1",
          "transcript_support_level": null,
          "aa_start": 406,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 1216,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912306.1"
        },
        {
          "aa_ref": "P",
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        {
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        {
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        {
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          ],
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          "gene_symbol": "RYK",
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          "hgvs_c": "n.435+16302C>T",
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          "transcript": "ENST00000484106.3",
          "protein_id": "ENSP00000487023.1",
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000484106.3"
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      ],
      "gene_symbol": "RYK",
      "gene_hgnc_id": 10481,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8726492524147034,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.731,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9726,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.13,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 9.602,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001005861.3",
          "gene_symbol": "RYK",
          "hgnc_id": 10481,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Pro551Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}