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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-14139250-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=14139250&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 14139250,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000306077.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.953C>T",
          "hgvs_p": "p.Ala318Val",
          "transcript": "NM_024334.3",
          "protein_id": "NP_077310.1",
          "transcript_support_level": null,
          "aa_start": 318,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 953,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": 1095,
          "cdna_end": null,
          "cdna_length": 3230,
          "mane_select": "ENST00000306077.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.953C>T",
          "hgvs_p": "p.Ala318Val",
          "transcript": "ENST00000306077.5",
          "protein_id": "ENSP00000303992.5",
          "transcript_support_level": 1,
          "aa_start": 318,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 953,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": 1095,
          "cdna_end": null,
          "cdna_length": 3230,
          "mane_select": "NM_024334.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000268279",
          "gene_hgnc_id": null,
          "hgvs_c": "n.188C>T",
          "hgvs_p": null,
          "transcript": "ENST00000608606.1",
          "protein_id": "ENSP00000476275.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 653,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.956C>T",
          "hgvs_p": "p.Ala319Val",
          "transcript": "NM_001407274.1",
          "protein_id": "NP_001394203.1",
          "transcript_support_level": null,
          "aa_start": 319,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 956,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": 1098,
          "cdna_end": null,
          "cdna_length": 3233,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.950C>T",
          "hgvs_p": "p.Ala317Val",
          "transcript": "NM_001407275.1",
          "protein_id": "NP_001394204.1",
          "transcript_support_level": null,
          "aa_start": 317,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 950,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 1092,
          "cdna_end": null,
          "cdna_length": 3227,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.950C>T",
          "hgvs_p": "p.Ala317Val",
          "transcript": "NM_001407276.1",
          "protein_id": "NP_001394205.1",
          "transcript_support_level": null,
          "aa_start": 317,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 950,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 1092,
          "cdna_end": null,
          "cdna_length": 3227,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.947C>T",
          "hgvs_p": "p.Ala316Val",
          "transcript": "NM_001407277.1",
          "protein_id": "NP_001394206.1",
          "transcript_support_level": null,
          "aa_start": 316,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 947,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": 1089,
          "cdna_end": null,
          "cdna_length": 3224,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.938C>T",
          "hgvs_p": "p.Ala313Val",
          "transcript": "NM_001407278.1",
          "protein_id": "NP_001394207.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": 1080,
          "cdna_end": null,
          "cdna_length": 3215,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.878C>T",
          "hgvs_p": "p.Ala293Val",
          "transcript": "NM_001407279.1",
          "protein_id": "NP_001394208.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 375,
          "cds_start": 878,
          "cds_end": null,
          "cds_length": 1128,
          "cdna_start": 1020,
          "cdna_end": null,
          "cdna_length": 3155,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "c.803C>T",
          "hgvs_p": "p.Ala268Val",
          "transcript": "NM_001407280.1",
          "protein_id": "NP_001394209.1",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 350,
          "cds_start": 803,
          "cds_end": null,
          "cds_length": 1053,
          "cdna_start": 945,
          "cdna_end": null,
          "cdna_length": 3080,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "n.*983C>T",
          "hgvs_p": null,
          "transcript": "ENST00000432444.2",
          "protein_id": "ENSP00000395617.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3484,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000268279",
          "gene_hgnc_id": null,
          "hgvs_c": "n.280C>T",
          "hgvs_p": null,
          "transcript": "ENST00000601399.3",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 787,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "n.589C>T",
          "hgvs_p": null,
          "transcript": "ENST00000713947.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2723,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM43",
          "gene_hgnc_id": 28472,
          "hgvs_c": "n.*983C>T",
          "hgvs_p": null,
          "transcript": "ENST00000432444.2",
          "protein_id": "ENSP00000395617.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3484,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "TMEM43",
      "gene_hgnc_id": 28472,
      "dbsnp": "rs11924644",
      "frequency_reference_population": 0.0010779507,
      "hom_count_reference_population": 21,
      "allele_count_reference_population": 1740,
      "gnomad_exomes_af": 0.000594448,
      "gnomad_genomes_af": 0.00571845,
      "gnomad_exomes_ac": 869,
      "gnomad_genomes_ac": 871,
      "gnomad_exomes_homalt": 13,
      "gnomad_genomes_homalt": 8,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.009778648614883423,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.115,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1348,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.5,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.392,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000306077.5",
          "gene_symbol": "TMEM43",
          "hgnc_id": 28472,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.953C>T",
          "hgvs_p": "p.Ala318Val"
        },
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000608606.1",
          "gene_symbol": "ENSG00000268279",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.188C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Arrhythmogenic right ventricular dysplasia 5,Cardiomyopathy,Cardiovascular phenotype,Sudden cardiac arrest,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2 B:8",
      "phenotype_combined": "not specified|Cardiovascular phenotype|Sudden cardiac arrest|Cardiomyopathy|not provided|Arrhythmogenic right ventricular dysplasia 5",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}