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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-142465177-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=142465177&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 142465177,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001184.4",
      "consequences": [
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6961T>A",
          "hgvs_p": "p.Phe2321Ile",
          "transcript": "NM_001184.4",
          "protein_id": "NP_001175.2",
          "transcript_support_level": null,
          "aa_start": 2321,
          "aa_end": null,
          "aa_length": 2644,
          "cds_start": 6961,
          "cds_end": null,
          "cds_length": 7935,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000350721.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001184.4"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6961T>A",
          "hgvs_p": "p.Phe2321Ile",
          "transcript": "ENST00000350721.9",
          "protein_id": "ENSP00000343741.4",
          "transcript_support_level": 1,
          "aa_start": 2321,
          "aa_end": null,
          "aa_length": 2644,
          "cds_start": 6961,
          "cds_end": null,
          "cds_length": 7935,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001184.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000350721.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "n.2145T>A",
          "hgvs_p": null,
          "transcript": "ENST00000513291.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000513291.2"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 40,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6808T>A",
          "hgvs_p": "p.Phe2270Ile",
          "transcript": "ENST00000936442.1",
          "protein_id": "ENSP00000606501.1",
          "transcript_support_level": null,
          "aa_start": 2270,
          "aa_end": null,
          "aa_length": 2593,
          "cds_start": 6808,
          "cds_end": null,
          "cds_length": 7782,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936442.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 40,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6769T>A",
          "hgvs_p": "p.Phe2257Ile",
          "transcript": "NM_001354579.2",
          "protein_id": "NP_001341508.1",
          "transcript_support_level": null,
          "aa_start": 2257,
          "aa_end": null,
          "aa_length": 2580,
          "cds_start": 6769,
          "cds_end": null,
          "cds_length": 7743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001354579.2"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 40,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6769T>A",
          "hgvs_p": "p.Phe2257Ile",
          "transcript": "ENST00000661310.1",
          "protein_id": "ENSP00000499589.1",
          "transcript_support_level": null,
          "aa_start": 2257,
          "aa_end": null,
          "aa_length": 2580,
          "cds_start": 6769,
          "cds_end": null,
          "cds_length": 7743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000661310.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 40,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6751T>A",
          "hgvs_p": "p.Phe2251Ile",
          "transcript": "ENST00000936443.1",
          "protein_id": "ENSP00000606502.1",
          "transcript_support_level": null,
          "aa_start": 2251,
          "aa_end": null,
          "aa_length": 2574,
          "cds_start": 6751,
          "cds_end": null,
          "cds_length": 7725,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936443.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6967T>A",
          "hgvs_p": "p.Phe2323Ile",
          "transcript": "XM_011512924.2",
          "protein_id": "XP_011511226.1",
          "transcript_support_level": null,
          "aa_start": 2323,
          "aa_end": null,
          "aa_length": 2646,
          "cds_start": 6967,
          "cds_end": null,
          "cds_length": 7941,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011512924.2"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6967T>A",
          "hgvs_p": "p.Phe2323Ile",
          "transcript": "XM_047448360.1",
          "protein_id": "XP_047304316.1",
          "transcript_support_level": null,
          "aa_start": 2323,
          "aa_end": null,
          "aa_length": 2612,
          "cds_start": 6967,
          "cds_end": null,
          "cds_length": 7839,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047448360.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6967T>A",
          "hgvs_p": "p.Phe2323Ile",
          "transcript": "XM_047448361.1",
          "protein_id": "XP_047304317.1",
          "transcript_support_level": null,
          "aa_start": 2323,
          "aa_end": null,
          "aa_length": 2598,
          "cds_start": 6967,
          "cds_end": null,
          "cds_length": 7797,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047448361.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 40,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.6775T>A",
          "hgvs_p": "p.Phe2259Ile",
          "transcript": "XM_011512925.2",
          "protein_id": "XP_011511227.1",
          "transcript_support_level": null,
          "aa_start": 2259,
          "aa_end": null,
          "aa_length": 2582,
          "cds_start": 6775,
          "cds_end": null,
          "cds_length": 7749,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011512925.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "c.293-5794T>A",
          "hgvs_p": null,
          "transcript": "ENST00000892891.1",
          "protein_id": "ENSP00000562950.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892891.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "n.1804T>A",
          "hgvs_p": null,
          "transcript": "ENST00000654170.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000654170.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "n.5749T>A",
          "hgvs_p": null,
          "transcript": "ENST00000656590.1",
          "protein_id": "ENSP00000499225.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000656590.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "n.3299T>A",
          "hgvs_p": null,
          "transcript": "ENST00000665483.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000665483.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "n.3464T>A",
          "hgvs_p": null,
          "transcript": "ENST00000666447.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000666447.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATR",
          "gene_hgnc_id": 882,
          "hgvs_c": "n.3693T>A",
          "hgvs_p": null,
          "transcript": "ENST00000666943.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000666943.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000295506",
          "gene_hgnc_id": null,
          "hgvs_c": "n.142+16895A>T",
          "hgvs_p": null,
          "transcript": "ENST00000730510.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000730510.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000244327",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*138T>A",
          "hgvs_p": null,
          "transcript": "ENST00000460977.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000460977.1"
        }
      ],
      "gene_symbol": "ATR",
      "gene_hgnc_id": 882,
      "dbsnp": "rs587783338",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.30390599370002747,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.09000000357627869,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.185,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0792,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.24,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.795,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.09,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001184.4",
          "gene_symbol": "ATR",
          "hgnc_id": 882,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD,Unknown",
          "hgvs_c": "c.6961T>A",
          "hgvs_p": "p.Phe2321Ile"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000730510.1",
          "gene_symbol": "ENSG00000295506",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.142+16895A>T",
          "hgvs_p": null
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000460977.1",
          "gene_symbol": "ENSG00000244327",
          "hgnc_id": null,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*138T>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}