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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-149032267-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=149032267&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 149032267,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_003071.4",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu",
          "transcript": "NM_003071.4",
          "protein_id": "NP_003062.2",
          "transcript_support_level": null,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000310053.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003071.4"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu",
          "transcript": "ENST00000310053.10",
          "protein_id": "ENSP00000308944.5",
          "transcript_support_level": 1,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003071.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000310053.10"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu",
          "transcript": "ENST00000392912.6",
          "protein_id": "ENSP00000376644.2",
          "transcript_support_level": 1,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392912.6"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2980A>T",
          "hgvs_p": "p.Met994Leu",
          "transcript": "ENST00000465259.5",
          "protein_id": "ENSP00000420745.1",
          "transcript_support_level": 1,
          "aa_start": 994,
          "aa_end": null,
          "aa_length": 1008,
          "cds_start": 2980,
          "cds_end": null,
          "cds_length": 3027,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000465259.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.3100A>T",
          "hgvs_p": "p.Met1034Leu",
          "transcript": "ENST00000911718.1",
          "protein_id": "ENSP00000581777.1",
          "transcript_support_level": null,
          "aa_start": 1034,
          "aa_end": null,
          "aa_length": 1048,
          "cds_start": 3100,
          "cds_end": null,
          "cds_length": 3147,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911718.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu",
          "transcript": "NM_001318935.2",
          "protein_id": "NP_001305864.1",
          "transcript_support_level": null,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318935.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu",
          "transcript": "NM_139048.3",
          "protein_id": "NP_620636.1",
          "transcript_support_level": null,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_139048.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu",
          "transcript": "ENST00000494055.5",
          "protein_id": "ENSP00000420429.1",
          "transcript_support_level": 2,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000494055.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2980A>T",
          "hgvs_p": "p.Met994Leu",
          "transcript": "NM_001318934.2",
          "protein_id": "NP_001305863.1",
          "transcript_support_level": null,
          "aa_start": 994,
          "aa_end": null,
          "aa_length": 1008,
          "cds_start": 2980,
          "cds_end": null,
          "cds_length": 3027,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318934.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2995A>T",
          "hgvs_p": "p.Met999Leu",
          "transcript": "XM_011513091.3",
          "protein_id": "XP_011511393.1",
          "transcript_support_level": null,
          "aa_start": 999,
          "aa_end": null,
          "aa_length": 1013,
          "cds_start": 2995,
          "cds_end": null,
          "cds_length": 3042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513091.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2995A>T",
          "hgvs_p": "p.Met999Leu",
          "transcript": "XM_011513092.3",
          "protein_id": "XP_011511394.1",
          "transcript_support_level": null,
          "aa_start": 999,
          "aa_end": null,
          "aa_length": 1013,
          "cds_start": 2995,
          "cds_end": null,
          "cds_length": 3042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513092.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2992A>T",
          "hgvs_p": "p.Met998Leu",
          "transcript": "XM_011513093.3",
          "protein_id": "XP_011511395.1",
          "transcript_support_level": null,
          "aa_start": 998,
          "aa_end": null,
          "aa_length": 1012,
          "cds_start": 2992,
          "cds_end": null,
          "cds_length": 3039,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513093.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2992A>T",
          "hgvs_p": "p.Met998Leu",
          "transcript": "XM_017007078.2",
          "protein_id": "XP_016862567.1",
          "transcript_support_level": null,
          "aa_start": 998,
          "aa_end": null,
          "aa_length": 1012,
          "cds_start": 2992,
          "cds_end": null,
          "cds_length": 3039,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017007078.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HLTF",
          "gene_hgnc_id": 11099,
          "hgvs_c": "c.2980A>T",
          "hgvs_p": "p.Met994Leu",
          "transcript": "XM_017007079.3",
          "protein_id": "XP_016862568.1",
          "transcript_support_level": null,
          "aa_start": 994,
          "aa_end": null,
          "aa_length": 1008,
          "cds_start": 2980,
          "cds_end": null,
          "cds_length": 3027,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017007079.3"
        }
      ],
      "gene_symbol": "HLTF",
      "gene_hgnc_id": 11099,
      "dbsnp": "rs762863740",
      "frequency_reference_population": 0.000002071397,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.0000020714,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.06927704811096191,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.066,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0977,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.37,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.964,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_003071.4",
          "gene_symbol": "HLTF",
          "hgnc_id": 11099,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2983A>T",
          "hgvs_p": "p.Met995Leu"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}