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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-15059458-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=15059458&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 15059458,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_022497.5",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "NM_022497.5",
          "protein_id": "NP_071942.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000253686.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_022497.5"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000253686.7",
          "protein_id": "ENSP00000253686.2",
          "transcript_support_level": 1,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_022497.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000253686.7"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000887322.1",
          "protein_id": "ENSP00000557381.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 169,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 510,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887322.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000449354.7",
          "protein_id": "ENSP00000390882.2",
          "transcript_support_level": 2,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000449354.7"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000939927.1",
          "protein_id": "ENSP00000609986.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939927.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000444840.6",
          "protein_id": "ENSP00000407733.2",
          "transcript_support_level": 2,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 129,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 390,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000444840.6"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000695323.1",
          "protein_id": "ENSP00000511804.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 112,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 339,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695323.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser",
          "transcript": "ENST00000695381.1",
          "protein_id": "ENSP00000511854.1",
          "transcript_support_level": null,
          "aa_start": 51,
          "aa_end": null,
          "aa_length": 102,
          "cds_start": 152,
          "cds_end": null,
          "cds_length": 309,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695381.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "c.134+5603A>G",
          "hgvs_p": null,
          "transcript": "ENST00000887323.1",
          "protein_id": "ENSP00000557382.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 108,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 327,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887323.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000420267.6",
          "protein_id": "ENSP00000396828.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000420267.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000447299.3",
          "protein_id": "ENSP00000402068.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000447299.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000474866.3",
          "protein_id": "ENSP00000511860.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000474866.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000496484.3",
          "protein_id": "ENSP00000511856.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000496484.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695334.2",
          "protein_id": "ENSP00000511812.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695334.2"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695375.1",
          "protein_id": "ENSP00000511848.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695375.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695376.1",
          "protein_id": "ENSP00000511849.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695376.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695377.1",
          "protein_id": "ENSP00000511850.1",
          "transcript_support_level": null,
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          "mane_select": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695377.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695378.1",
          "protein_id": "ENSP00000511851.1",
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          "aa_length": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695378.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695379.1",
          "protein_id": "ENSP00000511852.1",
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695379.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS25",
          "gene_hgnc_id": 14511,
          "hgvs_c": "n.152A>G",
          "hgvs_p": null,
          "transcript": "ENST00000695380.2",
          "protein_id": "ENSP00000511853.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000695380.2"
        },
        {
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      "frequency_reference_population": 0.000015501435,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 25,
      "gnomad_exomes_af": 0.00000958609,
      "gnomad_genomes_af": 0.000072224,
      "gnomad_exomes_ac": 14,
      "gnomad_genomes_ac": 11,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.08519488573074341,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.09000000357627869,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.08,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0909,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.45,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.13,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.09,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 2,
          "pathogenic_score": 1,
          "criteria": [
            "PP2",
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_022497.5",
          "gene_symbol": "MRPS25",
          "hgnc_id": 14511,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.152A>G",
          "hgvs_p": "p.Asn51Ser"
        },
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000698785.1",
          "gene_symbol": "ENSG00000289750",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.2912A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}