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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-158690264-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=158690264&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 158690264,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000486715.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.2011C>T",
          "hgvs_p": "p.Arg671Cys",
          "transcript": "NM_024996.7",
          "protein_id": "NP_079272.4",
          "transcript_support_level": null,
          "aa_start": 671,
          "aa_end": null,
          "aa_length": 751,
          "cds_start": 2011,
          "cds_end": null,
          "cds_length": 2256,
          "cdna_start": 2119,
          "cdna_end": null,
          "cdna_length": 6478,
          "mane_select": "ENST00000486715.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.2011C>T",
          "hgvs_p": "p.Arg671Cys",
          "transcript": "ENST00000486715.6",
          "protein_id": "ENSP00000419038.1",
          "transcript_support_level": 1,
          "aa_start": 671,
          "aa_end": null,
          "aa_length": 751,
          "cds_start": 2011,
          "cds_end": null,
          "cds_length": 2256,
          "cdna_start": 2119,
          "cdna_end": null,
          "cdna_length": 6478,
          "mane_select": "NM_024996.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.2068C>T",
          "hgvs_p": "p.Arg690Cys",
          "transcript": "NM_001308164.2",
          "protein_id": "NP_001295093.1",
          "transcript_support_level": null,
          "aa_start": 690,
          "aa_end": null,
          "aa_length": 770,
          "cds_start": 2068,
          "cds_end": null,
          "cds_length": 2313,
          "cdna_start": 2176,
          "cdna_end": null,
          "cdna_length": 6535,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.2068C>T",
          "hgvs_p": "p.Arg690Cys",
          "transcript": "ENST00000264263.9",
          "protein_id": "ENSP00000264263.5",
          "transcript_support_level": 5,
          "aa_start": 690,
          "aa_end": null,
          "aa_length": 770,
          "cds_start": 2068,
          "cds_end": null,
          "cds_length": 2313,
          "cdna_start": 2106,
          "cdna_end": null,
          "cdna_length": 3453,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1930C>T",
          "hgvs_p": "p.Arg644Cys",
          "transcript": "NM_001374355.1",
          "protein_id": "NP_001361284.1",
          "transcript_support_level": null,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1930,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": 2038,
          "cdna_end": null,
          "cdna_length": 3388,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1894C>T",
          "hgvs_p": "p.Arg632Cys",
          "transcript": "NM_001374356.1",
          "protein_id": "NP_001361285.1",
          "transcript_support_level": null,
          "aa_start": 632,
          "aa_end": null,
          "aa_length": 712,
          "cds_start": 1894,
          "cds_end": null,
          "cds_length": 2139,
          "cdna_start": 2002,
          "cdna_end": null,
          "cdna_length": 3352,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1786C>T",
          "hgvs_p": "p.Arg596Cys",
          "transcript": "NM_001374357.1",
          "protein_id": "NP_001361286.1",
          "transcript_support_level": null,
          "aa_start": 596,
          "aa_end": null,
          "aa_length": 676,
          "cds_start": 1786,
          "cds_end": null,
          "cds_length": 2031,
          "cdna_start": 2285,
          "cdna_end": null,
          "cdna_length": 3635,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1552C>T",
          "hgvs_p": "p.Arg518Cys",
          "transcript": "NM_001374358.1",
          "protein_id": "NP_001361287.1",
          "transcript_support_level": null,
          "aa_start": 518,
          "aa_end": null,
          "aa_length": 598,
          "cds_start": 1552,
          "cds_end": null,
          "cds_length": 1797,
          "cdna_start": 1660,
          "cdna_end": null,
          "cdna_length": 3010,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1444C>T",
          "hgvs_p": "p.Arg482Cys",
          "transcript": "NM_001374359.1",
          "protein_id": "NP_001361288.1",
          "transcript_support_level": null,
          "aa_start": 482,
          "aa_end": null,
          "aa_length": 562,
          "cds_start": 1444,
          "cds_end": null,
          "cds_length": 1689,
          "cdna_start": 1781,
          "cdna_end": null,
          "cdna_length": 3131,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1444C>T",
          "hgvs_p": "p.Arg482Cys",
          "transcript": "NM_001374360.1",
          "protein_id": "NP_001361289.1",
          "transcript_support_level": null,
          "aa_start": 482,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 1444,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": 1781,
          "cdna_end": null,
          "cdna_length": 3077,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.1327C>T",
          "hgvs_p": "p.Arg443Cys",
          "transcript": "NM_001374361.1",
          "protein_id": "NP_001361290.1",
          "transcript_support_level": null,
          "aa_start": 443,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 1327,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": 1664,
          "cdna_end": null,
          "cdna_length": 3014,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.417C>T",
          "hgvs_p": null,
          "transcript": "ENST00000477721.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 739,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.*651C>T",
          "hgvs_p": null,
          "transcript": "ENST00000478254.5",
          "protein_id": "ENSP00000417225.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.2034C>T",
          "hgvs_p": null,
          "transcript": "NR_164499.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3384,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.1974C>T",
          "hgvs_p": null,
          "transcript": "NR_164500.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3324,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.1519C>T",
          "hgvs_p": null,
          "transcript": "NR_164501.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2869,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.1998C>T",
          "hgvs_p": null,
          "transcript": "NR_164502.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3348,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "n.*651C>T",
          "hgvs_p": null,
          "transcript": "ENST00000478254.5",
          "protein_id": "ENSP00000417225.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GFM1",
          "gene_hgnc_id": 13780,
          "hgvs_c": "c.38-875C>T",
          "hgvs_p": null,
          "transcript": "ENST00000472383.1",
          "protein_id": "ENSP00000420272.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 16,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 51,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 656,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "GFM1",
      "gene_hgnc_id": 13780,
      "dbsnp": "rs201408725",
      "frequency_reference_population": 0.000033463388,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 54,
      "gnomad_exomes_af": 0.0000301047,
      "gnomad_genomes_af": 0.0000657289,
      "gnomad_exomes_ac": 44,
      "gnomad_genomes_ac": 10,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9383022785186768,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.805,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.6212,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.31,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.503,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 10,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PP3_Moderate,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 10,
          "benign_score": 0,
          "pathogenic_score": 10,
          "criteria": [
            "PP3_Moderate",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000486715.6",
          "gene_symbol": "GFM1",
          "hgnc_id": 13780,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2011C>T",
          "hgvs_p": "p.Arg671Cys"
        }
      ],
      "clinvar_disease": "Hepatoencephalopathy due to combined oxidative phosphorylation defect type 1,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:8 LP:1",
      "phenotype_combined": "not provided|Hepatoencephalopathy due to combined oxidative phosphorylation defect type 1",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}