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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-179875404-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=179875404&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 179875404,
      "ref": "C",
      "alt": "T",
      "effect": "start_lost",
      "transcript": "NM_001256756.2",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.579G>A",
          "hgvs_p": "p.Met193Ile",
          "transcript": "NM_016559.3",
          "protein_id": "NP_057643.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 579,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000467460.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016559.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.579G>A",
          "hgvs_p": "p.Met193Ile",
          "transcript": "ENST00000467460.6",
          "protein_id": "ENSP00000419975.1",
          "transcript_support_level": 1,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 579,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_016559.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000467460.6"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.573G>A",
          "hgvs_p": "p.Met191Ile",
          "transcript": "ENST00000263962.12",
          "protein_id": "ENSP00000263962.8",
          "transcript_support_level": 1,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 624,
          "cds_start": 573,
          "cds_end": null,
          "cds_length": 1875,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263962.12"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.474G>A",
          "hgvs_p": "p.Met158Ile",
          "transcript": "ENST00000485199.5",
          "protein_id": "ENSP00000418440.1",
          "transcript_support_level": 1,
          "aa_start": 158,
          "aa_end": null,
          "aa_length": 591,
          "cds_start": 474,
          "cds_end": null,
          "cds_length": 1776,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000485199.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001256756.2",
          "protein_id": "NP_001243685.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256756.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349399.2",
          "protein_id": "NP_001336328.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349399.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349401.2",
          "protein_id": "NP_001336330.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349401.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349404.2",
          "protein_id": "NP_001336333.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349404.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349406.2",
          "protein_id": "NP_001336335.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349406.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349408.2",
          "protein_id": "NP_001336337.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349408.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349409.2",
          "protein_id": "NP_001336338.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349409.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "NM_001349410.2",
          "protein_id": "NP_001336339.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349410.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "ENST00000468741.5",
          "protein_id": "ENSP00000418665.1",
          "transcript_support_level": 2,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000468741.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "ENST00000496721.5",
          "protein_id": "ENSP00000418746.1",
          "transcript_support_level": 3,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 69,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 211,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000496721.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "ENST00000491640.5",
          "protein_id": "ENSP00000419790.1",
          "transcript_support_level": 4,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 33,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000491640.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "start_lost"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.3G>A",
          "hgvs_p": "p.Met1?",
          "transcript": "XM_011512891.3",
          "protein_id": "XP_011511193.1",
          "transcript_support_level": null,
          "aa_start": 1,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 3,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011512891.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.744G>A",
          "hgvs_p": "p.Met248Ile",
          "transcript": "NM_001349386.2",
          "protein_id": "NP_001336315.1",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": 744,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349386.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.651G>A",
          "hgvs_p": "p.Met217Ile",
          "transcript": "NM_001349387.2",
          "protein_id": "NP_001336316.1",
          "transcript_support_level": null,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 650,
          "cds_start": 651,
          "cds_end": null,
          "cds_length": 1953,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349387.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.651G>A",
          "hgvs_p": "p.Met217Ile",
          "transcript": "NM_001349388.2",
          "protein_id": "NP_001336317.1",
          "transcript_support_level": null,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 650,
          "cds_start": 651,
          "cds_end": null,
          "cds_length": 1953,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349388.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX5L",
          "gene_hgnc_id": 30024,
          "hgvs_c": "c.645G>A",
          "hgvs_p": "p.Met215Ile",
          "transcript": "NM_001349389.2",
          "protein_id": "NP_001336318.1",
          "transcript_support_level": null,
          "aa_start": 215,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 645,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349389.2"
        },
        {
          "aa_ref": "M",
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      "gene_symbol": "PEX5L",
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      "dbsnp": "rs1752016864",
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      "gnomad_exomes_af": null,
      "gnomad_genomes_af": 0.00000657549,
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      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.21786922216415405,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
      "alphamissense_score": 0.0876,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.19,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.139,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PVS1_Supporting,PM2",
      "acmg_by_gene": [
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          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PVS1_Supporting",
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001256756.2",
          "gene_symbol": "PEX5L",
          "hgnc_id": 30024,
          "effects": [
            "start_lost"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.3G>A",
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        {
          "score": 0,
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            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000466064.1",
          "gene_symbol": "PEX5L-AS1",
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          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}